Difference between revisions of "Team:Chalmers-Gothenburg/Protocols"

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         <a class="dropdown-item" href="https://2018.igem.org/Team:Chalmers-Gothenburg/InterLab" style="color:#16181b">Interlab</a>
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<h2> Protocols</h2>
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        <h2>
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          Protocols for PCR
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        </h2>
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf" target="blank">Thermo Scientific DreamTaq DNA Polymerase</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf" target="blank">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br>
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          <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Chalmers-Gothenburg--Takara_PrimeSTAR_HS_DNA_Polymerase.pdf" target="blank">PrimeSTAR HS DNA Polymerase</a><br>
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          <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf" target="blank">Fusion PCR</a>
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          Protocols for purification
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf" target="blank">Thermo Scientific GeneJET PCR Purification Kit</a><br>
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          <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf" target="blank">Thermo Scientific GeneJET Gel Extraction Kit</a>
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          Protocols for digestion and assembly
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          <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf" target="blank">FastDigest Restriction Enzymes</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/9/9d/T--Chalmers_Gothenburg--Protocols_gibson_assembly.pdf" target="blank">Gibson Assembly Master Mix</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf" target="blank">DNA Insert Ligation into Vector DNA</a>
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        <h2>
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          Protocols for <i>Saccharomyces cerevisiae</i>
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          <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf" target="blank">Transformation (Chemical)</a><br>
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          <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf" target="blank">Transformation (Quick version)</a><br>
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          <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf" target="blank">Isolation of genomic DNA</a>
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        <h2>
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          Protocols for <i>Escherichia coli</i>
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          <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf" target="blank">Transformation (Heat shock)</a><br>
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          <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf" target="blank">Thermo Scientific GeneJET Plasmid Miniprep Kit</a>
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        <h2>
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          Protocols for Biobricks
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf" target="blank">BioBricks Assembly Protocol</a>
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          Protocols for Interlab study
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        <p class="text">
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          <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf" target="blank">Interlab study</a>
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<footer>
 
<footer>
 
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   <h4>
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   <h4 style="font-size:12pt;">
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     Contact information
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     CONTACT INFORMATION:
 
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     <p style="font-size:10pt;">
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       igemgothenburg@gmail.com   
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       CHALMERS University of Technology, Gothenburg, Sweden, igemgothenburg@gmail.com   
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</p>
 
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         <a href="https://www.facebook.com/chalmersigem">Facebook</a>   
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         <a style="font-size:10pt;" href="https://www.facebook.com/chalmersigem">Facebook</a>   
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         <a href="https://www.instagram.com/chalmersigem">Instagram</a>   
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         <a style="font-size:10pt;" href="https://www.instagram.com/chalmersigem">Instagram</a>   
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         <a href="https://twitter.com/igemchalmers">Twitter</a>   
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         <a style="font-size:10pt;" href="https://twitter.com/igemchalmers">Twitter</a>   
 
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Latest revision as of 19:51, 17 October 2018

Interlab - iGEM Chalmers-Gothenburg 2018

Protocols


Workflow Overview

In the figure below an overview of the cloning process, shared by all project parts, is presented.

Logo
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Figure 1: In this picture a schematic overview of the cloning process is presented, from amplification oof genes and digestion pasmid to thransformation of plasmid into yeast.

Protocols for PCR

Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR

Protocols for purification

Thermo Scientific GeneJET PCR Purification Kit
Thermo Scientific GeneJET Gel Extraction Kit

Protocols for digestion and assembly

FastDigest Restriction Enzymes
Gibson Assembly Master Mix
DNA Insert Ligation into Vector DNA

Protocols for Saccharomyces cerevisiae

Transformation (Chemical)
Transformation (Quick version)
Isolation of genomic DNA

Protocols for Escherichia coli

Transformation (Heat shock)
Thermo Scientific GeneJET Plasmid Miniprep Kit

Protocols for Biobricks

BioBricks Assembly Protocol

Protocols for Interlab study

Interlab study