Difference between revisions of "Team:Munich/assembly3.html"
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<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> T7 DNA: ca. 500 ng | + | <td> T7 DNA: ca. 500 ng <br> |
3S Phage: 330 ng <br> | 3S Phage: 330 ng <br> | ||
Self-produced extract: E10 | Self-produced extract: E10 | ||
Revision as of 20:04, 17 October 2018
T7 and 3S Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie Schönberg von Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng 3S Phage: 330 ng Self-produced extract: E10 |
| Results: | T7 Phage: 1,6*10^8 PFU/mL 3S: did not work out. Decided to redo the experiment. |
T7 Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22| Participants: | Brigit Tunaj, Sophie Schönberg von Schönberg |
| Protocol: | Phage T7 Assembly TX-TL, Agar overlay plaque assay |
| Notes: | T7 DNA: ca. 500 ng, |
| Results: | T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL |