Difference between revisions of "Team:Munich/chiversions.html"
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<figure class="figure"> | <figure class="figure"> | ||
<img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" "> | <img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" "> | ||
| − | <figcaption class="figure-caption"> | + | <figcaption class="figure-caption">We use 2 Log ladder from NEB as the marker</figcaption> |
</figure> | </figure> | ||
Revision as of 20:14, 17 October 2018
PCR amplify linear mtq2
2018/05/28 - 2018/05/29| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel Purification |
| Notes: | VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) |
| Results: | Worked (PIC) |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly
2018/06/04 - 2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel purification, Gibson Assembly |
| Notes: | Fragments were amplified, then purified and Gibson Assembly was performed. |
| Results: | No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly. |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR
2018/06/11 - 2018/06/14| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Fragments were amplified, then purified and overlap PCR was performed. |
| Results: | The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples. |
PCR to amplify Mtq2
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | PCR |
| Notes: | Primers: VF2, VR |
| Results: | No bands visible: redo.
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