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Revision as of 20:23, 17 October 2018

Bioremediation of Sour Crude Oil Waste using Cyanobacteria




May
Week 1-4
Week 1 April 30 - May 6
Week 2 May 7 - May 13
Week 3 May 14 - May 20
Week 4 May 21 - May 27
Week 5 May 28 – June 3
Week 6 June 4 – June 10
Week 7 June 11 – June 17
Week 8 June 18 – June 24
Week 9 June 25 – July 1

WEEK 1 (April 30 - May 6)

  • 5 May 2018

    BG-11 medium was prepared.

    Inoculation of the BG-11 medium with cyanobacteria (Synechococcus elongatus PCC 7942) was done.



WEEK 2 (May 7 - May 13)

  • 11 May 2018

    BG-11 agar plates were made.

    Cyanobacteria were plated onto BG-11 agar plates from liquid culture



WEEK 3 (May 14 - May 20)

  • 14 May 2018

    The new subculture of cyanobacteria was prepared from old liquid culture.

    The old liquid culture of cyanobacteria was plated onto BG -11 agar plate.

  • 16 May 2018

    BG-11 agar plates were prepared.

    Cyanobacteria from liquid culture were plated onto the BG-11 agar plates.



WEEK 4 (May 21 - May 27)

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured

    Liquid cultures of cyanobacteria were checked.

    The new subculture of cyanobacteria was prepared from old liquid culture.



WEEK 5 (May 28 – June 3)

  • 28 May 2018

    OD of liquid cyanobacteria culture was measured.

    Liquid cultures of cyanobacteria were checked.

  • 30 May 2018

    LB liquid medium and LB agar plates were prepared.

  • 31 May 2018

    Competent E.coli (TOP10) cells were prepared.



WEEK 6 (June 4 – June 10)

  • 4 June 2018

    New competent TOP10 cells were prepared.

    Spectinomycin stock solution 1000X was prepared.

    LB agar plates with spectinomycin were prepared.

  • 5 June 2018

    Transformation of E.coli (TOP10) with empty pSyn_6 vector (marker gene: spectinomycin resistance gene) was done.

    Transformed E.coli cells were plated on LB agar plates with spectinomycin.

  • 6 June 2018

    LB liquid medium with spectinomycin was prepared.

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 7 June 2018

    Plasmids from the transformed TOP10 were extracted by MiraPrep.

    Extracted plasmid samples were measured by Nanodrop.

    Samples 260/280 260/230 Concentration
    1 1.76 1.53 63.96 ng/ul
    2 1.96 2.29 1953 ng/u

    Gel Electrophoresis was done.

  • 8 June 2018

    OD of liquid cyanobacteria culture was measured. OD: 0.8.

    BG-11 agar plates with spectinomycin were prepared.



WEEK 7 (June 11 – June 17)

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 12 June 2018

    MiraPrep was done.

    Extracted plasmid samples were measured by Nanodrop.

    Samples 260/280 260/230 Concentration
    1 1.90 2.38 1600 ng/ul

    Gel Electrophoresis was done.

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

  • 11 June 2018

    Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured



WEEK 5

  • 25 May 2018

    OD of liquid cyanobacteria culture was measured

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