Difference between revisions of "Team:Munich/deletioncasette.html"
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<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td> | + | <td> We thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |
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| + | <figure class="figure"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/c/c6/T--munich--20180605_CAT-KO.tif " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption"></figcaption> | ||
| + | </figure> | ||
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</td> | </td> | ||
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Revision as of 20:27, 17 October 2018
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
2018/05/28| Participants: | Dominic Schwarz |
| Protocol: | Electro-transformation |
| Notes: | No notes. |
| Results: | No colonies, no growth; We decided to use a resistance cassette from pSB1C3 without FRT sites. |
Amplifying a selection cassette from pSB1C3
2018/05/29| Participants: | Enikö Baligács |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Primer with 50bp homology regions ????, TA: 67°C, Elongation: 45s |
| Results: | We thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments.
|