Difference between revisions of "Team:HZAU-China/Collaborations"

 
(23 intermediate revisions by 5 users not shown)
Line 303: Line 303:
 
             /* right: 2%; */
 
             /* right: 2%; */
 
             /* top: 90px; */
 
             /* top: 90px; */
−
             padding: 20px 3%;
+
             padding: 50px 3%;
 
             float: right;
 
             float: right;
 
             /* position: relative; */
 
             /* position: relative; */
Line 343: Line 343:
  
 
         .h1 {
 
         .h1 {
−
            height: 80px;
+
             line-height: 55px;
−
             line-height: 80px;
+
 
             font-weight: bold;
 
             font-weight: bold;
 
             height:auto;
 
             height:auto;
Line 494: Line 493:
 
     <!-- 滚动菜单栏CSS -->
 
     <!-- 滚动菜单栏CSS -->
 
     <style>
 
     <style>
−
            #float01 {}
+
        #float01 {}
−
   
+
 
−
            #float02 {}
+
        #float02 {}
−
   
+
 
−
            div.floatCtro {
+
        div.floatCtro {
−
                width: 100%;
+
            width: 100%;
−
                /* height: auto; */
+
            /* height: auto; */
−
                margin: 0;
+
            margin: 0;
−
                position: relative;
+
            position: relative;
−
                background: #fff;
+
            background: #fff;
−
   
+
 
 +
        }
 +
 
 +
        .daohangyi {
 +
            display: block;
 +
            width: 100%;
 +
            /* height: auto; */
 +
            /* text-align: left !important; */
 +
            color: #ffffff !important;
 +
            font-size: 16px;
 +
            padding:0;
 +
            background-color: #323643;
 +
            border-bottom: 1px solid black;
 +
            padding:0 3%;
 +
            text-decoration: none !important;
 +
        }
 +
 
 +
        div.floatCtro .daohanger {
 +
            width: 100%;
 +
            text-align: left !important;
 +
            height: auto;
 +
            line-height: 25px;
 +
            font-family: Arial;
 +
            font-size: 14px !important;
 +
            color: #676767;
 +
            margin: 0;
 +
            padding:10px 8%;
 +
            cursor: pointer;
 +
            background: #fff;
 +
        }
 +
 
 +
        div.floatCtro a {
 +
            display: inline-block;
 +
            display: none;
 +
            width: 100%;
 +
            height: 40px;
 +
            padding:10px 8%;
 +
            /* margin: 10px 0 0 0; */
 +
            background: #FFF;
 +
            color: #000 !important;
 +
            vertical-align: middle;
 +
            cursor: pointer;
 +
        }
 +
 
 +
        div.floatCtro a span {
 +
            display: block;
 +
            height: auto;
 +
            text-align: left !important;
 +
            line-height: 18px;
 +
            font-family: Arial;
 +
            font-size: 16px;
 +
        }
 +
 
 +
        div.floatCtro a:hover {
 +
            /* background: #76b39d; */
 +
            color: #EA0D04 !important;
 +
            zoom: 1;
 +
        }
 +
 
 +
        /* .cebian a:hover {
 +
            color: #f6eee0 !important;
 +
        } */
 +
 
 +
        div.floatCtro .daohanger:hover {
 +
            /* background: #76b39d; */
 +
            color: #1fa67a;
 +
            text-decoration: underline !important;
 +
        }
 +
 
 +
        div.floatCtro .daohanger.cur {
 +
            /* background: #1fa67a; */
 +
            color: #1fa67a;
 +
        }
 +
 
 +
        .biaoti {
 +
            display: inline-block;
 +
            margin: 8px 0;
 +
            /* width: 50%; */
 +
            /* margin-left: 6%; */
 +
            /* text-align: center; */
 +
            text-decoration: none !important;
 +
        }
 +
 
 +
        .daohangyi img {
 +
            display: inline-block;
 +
            width: 12px;
 +
            height: 12px;
 +
            vertical-align: middle;
 +
            background-size: 100% 100%;
 +
 
 +
        }
 +
        .cebian div:hover img{
 +
            transform: rotateY(180deg);           
 +
            -webkit-transform: rotateY(180deg);           
 +
            -moz-transform: rotateY(180deg);           
 +
            -o-transform: rotateY(180deg);           
 +
            -ms-transform: rotateY(180deg);
 +
            transition: all 0.5s ease-in-out;           
 +
            -webkit-transition: all 0.5s ease-in-out;           
 +
            -moz-transition: all 0.5s ease-in-out;           
 +
            -o-transition: all 0.5s ease-in-out;
 +
        }
 +
        .cebian a:hover img{
 +
            transform: rotate(360deg);           
 +
            -webkit-transform: rotate(360deg);           
 +
            -moz-transform: rotate(360deg);           
 +
            -o-transform: rotate(360deg);           
 +
            -ms-transform: rotate(360deg);
 +
            transition: all 0.5s ease-in-out;           
 +
            -webkit-transition: all 0.5s ease-in-out;           
 +
            -moz-transition: all 0.5s ease-in-out;           
 +
            -o-transition: all 0.5s ease-in-out;
 +
        }
 +
 
 +
        @media screen and (max-width: 1200px) {           
 +
         
 +
            .cebian {
 +
            width: 15%;
 
             }
 
             }
−
   
+
 
 +
            .zhengwen {
 +
            margin: 20px 20px 0 0;
 +
 
 
             .daohangyi {
 
             .daohangyi {
−
                display: block;
+
                 font-size: 14px;
−
                width: 100%;
+
−
                /* height: auto; */
+
−
                /* text-align: left !important; */
+
−
                color: #ffffff !important;
+
−
                 font-size: 16px;
+
−
                padding:0;
+
−
                background-color: #323643;
+
−
                border-bottom: 1px solid black;
+
−
                padding:0 3%;
+
−
                text-decoration: none !important;
+
 
             }
 
             }
−
   
+
             .daohangyi img {
−
             div.floatCtro .daohanger {
+
−
                width: 100%;
+
−
                text-align: left !important;
+
−
                height: auto;
+
−
                line-height: 25px;
+
−
                font-family: Arial;
+
−
                font-size: 14px !important;
+
−
                color: #676767;
+
−
                margin: 0;
+
−
                padding:10px 8%;
+
−
                cursor: pointer;
+
−
                background: #fff;
+
−
            }
+
−
   
+
−
            div.floatCtro a {
+
−
                display: inline-block;
+
 
                 display: none;
 
                 display: none;
−
                width: 100%;
+
             }
−
                height: 40px;
+
        }
−
                padding:10px 8%;
+
 
−
                /* margin: 10px 0 0 0; */
+
        @media screen and (max-width: 900px) {
−
                background: #FFF;
+
 
−
                color: #000 !important;
+
−
                vertical-align: middle;
+
−
                cursor: pointer;
+
−
             }
+
−
   
+
−
            div.floatCtro a span {
+
−
                display: block;
+
−
                height: auto;
+
−
                text-align: left !important;
+
−
                line-height: 20px;
+
−
                font-family: Arial;
+
−
                font-size: 16px;
+
−
            }
+
−
   
+
−
            div.floatCtro a:hover {
+
−
                /* background: #76b39d; */
+
−
                color: #EA0D04 !important;
+
−
                zoom: 1;
+
−
            }
+
−
   
+
−
            /* .cebian a:hover {
+
−
                color: #f6eee0 !important;
+
−
            } */
+
−
   
+
−
            div.floatCtro .daohanger:hover {
+
−
                /* background: #76b39d; */
+
−
                color: #1fa67a;
+
−
                text-decoration: underline !important;
+
−
            }
+
−
   
+
−
            div.floatCtro .daohanger.cur {
+
−
                /* background: #1fa67a; */
+
−
                color: #1fa67a;
+
−
            }
+
−
   
+
−
            .biaoti {
+
−
                line-height: 40px;
+
−
                width: 50%;
+
−
                /* margin-left: 6%; */
+
−
                text-align: center;
+
−
                text-decoration: none !important;
+
−
            }
+
−
   
+
 
             .daohangyi img {
 
             .daohangyi img {
−
                 display: inline-block;
+
                 display: none;
−
                width: 12px;
+
             }
−
                height: 12px;
+
              
−
                vertical-align: middle;
+
             .laoshi img {
−
                background-size: 100% 100%;
+
                 height: 30vh;
−
   
+
−
             }
+
−
             .cebian div:hover img{
+
−
                transform: rotateY(180deg);           
+
−
                -webkit-transform: rotateY(180deg);           
+
−
                -moz-transform: rotateY(180deg);           
+
−
                -o-transform: rotateY(180deg);           
+
−
                -ms-transform: rotateY(180deg);
+
−
                transition: all 0.5s ease-in-out;           
+
−
                -webkit-transition: all 0.5s ease-in-out;           
+
−
                -moz-transition: all 0.5s ease-in-out;           
+
−
                -o-transition: all 0.5s ease-in-out;
+
−
            }
+
−
             .cebian a:hover img{
+
−
                 transform: rotate(360deg);           
+
−
                -webkit-transform: rotate(360deg);           
+
−
                -moz-transform: rotate(360deg);           
+
−
                -o-transform: rotate(360deg);           
+
−
                -ms-transform: rotate(360deg);
+
−
                transition: all 0.5s ease-in-out;           
+
−
                -webkit-transition: all 0.5s ease-in-out;           
+
−
                -moz-transition: all 0.5s ease-in-out;           
+
−
                -o-transition: all 0.5s ease-in-out;
+
 
             }
 
             }
−
   
+
        }
−
            @media screen and (max-width: 1200px) {           
+
     </style>
−
             
+
−
                .cebian {
+
−
                width: 15%;
+
−
                }
+
−
      
+
−
                .zhengwen {
+
−
                margin: 20px 20px 0 0;
+
−
   
+
−
                .daohangyi {
+
−
                    font-size: 14px;
+
−
                }
+
−
                .daohangyi img {
+
−
                    display: none;
+
−
                } 
+
−
            }
+
−
   
+
−
            @media screen and (max-width: 900px) {
+
−
   
+
−
                .daohangyi img {
+
−
                    display: none;
+
−
                } 
+
−
               
+
−
                .laoshi img {
+
−
                    height: 30vh;
+
−
                }
+
−
            }
+
−
        </style>
+
  
  
Line 652: Line 652:
 
     <!-- 导航栏 -->
 
     <!-- 导航栏 -->
 
     <nav class="daohang">
 
     <nav class="daohang">
−
            <a href="https://2018.igem.org/Team:HZAU-China"><img src="https://static.igem.org/mediawiki/2018/7/75/T--HZAU-China--logo-nav.png"
+
        <a href="https://2018.igem.org/Team:HZAU-China"><img src="https://static.igem.org/mediawiki/2018/7/75/T--HZAU-China--logo-nav.png"
−
                    class="logo-daohang"></a>
+
                class="logo-daohang"></a>
−
            <ul class="caidan">
+
        <ul class="caidan">
−
                <li class="hiLight shortName">
+
            <li class="hiLight shortName">
−
                    <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China">
+
                <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China">
−
                        <span>Project</span>
+
                    <span>Project</span>
−
                        <span class="xsjPic"></span>
+
                    <span class="xsjPic"></span>
−
                    </a>
+
                </a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Design">Design</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Design">Design</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Results">Results</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Results">Results</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Demonstrate">Demonstrate</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Demonstrate">Demonstrate</a>
−
                </li>
+
            </li>
−
                <li class="hiLight shortName">
+
            <li class="hiLight shortName">
−
                    <a class="nav_head" href="#">
+
                <a class="nav_head" href="#">
−
                        <span>Wetlab</span>
+
                    <span>Wetlab</span>
−
                        <span class="xjtPic"></span>
+
                    <span class="xjtPic"></span>
−
                    </a>
+
                </a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Notebook">Notebook</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Notebook">Notebook</a>
−
   
+
 
−
                </li>
+
            </li>
−
                <li class="hiLight shortName">
+
            <li class="hiLight shortName">
−
                    <a class="nav_head" href="#">
+
                <a class="nav_head" href="#">
−
                        <span>Drylab</span>
+
                    <span>Drylab</span>
−
                        <span class="xjtPic"></span>
+
                    <span class="xjtPic"></span>
−
                    </a>
+
                </a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Model">Model</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Model">Model</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Software">Software</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Software">Software</a>
−
                </li>
+
            </li>
−
                <li class="hiLight longName">
+
            <li class="hiLight longName">
−
                    <a class="nav_head" href="#">
+
                <a class="nav_head" href="#">
−
                        <span>Human&nbsp;Practices</span>
+
                    <span>Human&nbsp;Practices</span>
−
                        <span class="xjtPic"></span>
+
                    <span class="xjtPic"></span>
−
                    </a>
+
                </a>
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                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
−
                </li>
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                <li class="hiLight shortName">
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            <li class="hiLight shortName">
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                    <a class="nav_head" href="#">
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                <a class="nav_head" href="#">
−
                        <span>About Us</span>
+
                    <span>About Us</span>
−
                        <span class="xjtPic"></span>
+
                    <span class="xjtPic"></span>
−
                    </a>
+
                </a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Team">Team</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Team">Team</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Attributions">Attributions</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Attributions">Attributions</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
−
                </li>
+
            </li>
−
                <li class="hiLight shortName">
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            <li class="hiLight shortName">
−
                    <a class="nav_head" href="#">
+
                <a class="nav_head" href="#">
−
                        <span>Parts</span>
+
                    <span>Parts</span>
−
                        <span class="xjtPic"></span>
+
                    <span class="xjtPic"></span>
−
                    </a>
+
                </a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Basic_Part">Basic</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Basic_Part">Basic</a>
−
                    <a class="item" href="https://2018.igem.org/Team:HZAU-China/Composite_Part">Composite</a>
+
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Composite_Part">Composite</a>
−
                </li>
+
            </li>
−
            </ul>
+
        </ul>
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            <div class="shade"></div>
+
        <div class="shade"></div>
−
        </nav>
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    </nav>
 
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             <div id="float01" class="cur">
 
             <div id="float01" class="cur">
−
                 <div class="h1">Collaboration with WHU-China and SDU-CHINA</div>
+
                 <div class="h1">Collaboration with WHU-China
−
                 <p>Optogenetic tools have broad prospects on synthetic biology, and the CcaS-CcaR system, which is
+
                    and SDU-CHINA</div>
−
                     developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to
+
                 <p>Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is
 +
                     developed from <i>cyanobacteria</i> has made obvious achievements, thus more and more teams are
 +
                    willing to
 
                     use this system in their own projects. In the past two years, we have spent a great deal of time
 
                     use this system in their own projects. In the past two years, we have spent a great deal of time
−
                     using it in our projects and accumulated a lot of experience(see more information in our previous
+
                     using it in our projects and accumulated a lot of experience (see more information in our previous
−
                     wikis ). This year, we collaborated with WHU-China and SDU-CHINA who use this system in project on
+
                     wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in their projects and
−
                     sharing the experience how we regulate the expression of CcaS and CcaR protein to control this
+
                     sharing the experience how we regulated the expression of CcaS and CcaR protein to control this
−
                     optogenetic tools and what we achieved
+
                     optogenetic tool and what we achieved.
 
                 </p>
 
                 </p>
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 60%; margin: 30px auto">
 +
                            <a href="https://2018.igem.org/Team:WHU-China/Collaborations"><img src="https://static.igem.org/mediawiki/2018/9/9a/T--WHU-China--wiki-Collabration_whu.png"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 60%; margin: 30px auto">
 +
                            <a href="https://2018.igem.org/Team:SDU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/c/c5/T--HZAU-China--SDU.png"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                </div>
  
 
             </div>
 
             </div>
 
             <div id="float02">
 
             <div id="float02">
−
                 <div class="h1">Experience sharing with Worldshaper</div>
+
                 <div class="h1">Experience sharing with Worldshaper-Wuhan</div>
−
                 <p>As a dynamic high school team, Worldshaper demonstrates great enthusiasm in this competition. They
+
                 <p>We cooperated with the high school team, Worldshaper-Wuhan. We shared some information about our
−
                     visited our laboratory and we shared them our experience of iGEM competition in the past years with
+
                     project and had several
−
                     relaxed discussion, including preparations before competition, some experimental techniques, notes
+
                    discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines.
−
                     of standardization and guiding them to do multiple cell lines
+
                     LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and
 +
                    therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if
 +
                     the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not.
 +
                    Secondly, we want to explore its applicability for which this biomarker can be widely utilized to
 +
                    detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS has specificity and
 +
                    universality. There were significant expression differences among 293T (the healthy cell), 769-P
 +
                    (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P
 +
                    and 786-O were higher than that of 293T, showing specificity and universality of IL7-AS. Through our
 +
                    team's suggestions and Worldshaper-Wuhan's experiment, their project improved and we also got a new
 +
                    biomarker
 +
                    and therapeutic target for future projects.
 
                 </p>
 
                 </p>
 +
                <div style="width: 20%; margin: 30px auto">
 +
                    <a href="https://2018.igem.org/Team:Worldshaper-Wuhan/Collaborations"><img src="https://static.igem.org/mediawiki/2018/b/b6/T--HZAU-China--WSWH.png"
 +
                            width=100% alt=""></a>
 +
                </div>
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-4 col-lg-4">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/0/02/T--HZAU-China--WSWH1.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-4 col-lg-4">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/9/97/T--HZAU-China--WSWH2.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-4 col-lg-4">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/3/35/T--HZAU-China--WSWH3.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
  
 
             </div>
 
             </div>
 
             <div id="float03">
 
             <div id="float03">
 
                 <div class="h1">Help from NAU-CHINA</div>
 
                 <div class="h1">Help from NAU-CHINA</div>
−
                 <p>After the information we got from meetings and public investigation, we thought it’s necessary to
+
 
−
                    enhance the targeting of engineered salmonella we used, we designed OmpA-RGD protein to achieve
+
                 <div class="row">
−
                    this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3
+
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
−
                    (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called
+
                        <div style="width: 100%; margin: 30px auto; padding: 0 20px;">
−
                    MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with
+
                            <p>After the information we got from meetings and public investigation, we thought it’s
−
                    the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of
+
                                necessary to
−
                    Biochemistry and Cell Biology.
+
                                enhance the targeting of engineered <i>Salmonella</i> we used, we designed a fuse
 +
                                protein which
 +
                                called OmpA-RGD to achieve
 +
                                this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to
 +
                                alpha v beta 3
 +
                                (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line
 +
                                called
 +
                                MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety
 +
                                experiments, with
 +
                                the great help of NAU-CHINA , we had channels to get this cell line from Shanghai
 +
                                Institute of
 +
                                Biochemistry and Cell Biology.
 +
                            </p>
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 80%; margin: 30px auto; ">
 +
                            <a href="https://2018.igem.org/Team:NAU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/8/88/T--HZAU-China--NAU.png"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 
 +
                <div class="h1">Collaboration with HUST-China</div>
 +
                <p>When we submitted our parts, we got the help of HUST-China. HUST-China
 +
                    helped us freeze the plasmid into dry powder in 96 well plate format, label our plate with our team name and
 +
                    submission number, and eventually send it by express to the agency in China. We are extremely grateful for their help.
 
                 </p>
 
                 </p>
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; box-shadow: 0 1px 3px #676767; background-color: #333333; ">
 +
                            <a href="https://2018.igem.org/Team:HUST-China/Collaborations"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--HZAU-China--HUST1.jpg"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/0/06/T--HZAU-China--HUST.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
 +
                </div>
 +
 +
                <div class="h1">EurAisan Meetup</div>
 +
                <p>We also took part in the iGEM EurAisan Meetup, where we shared our ideas and learned a lot. At the
 +
                    meeting, we had friendly communications with many iGEMers and gained a lot of advice during this
 +
                    meetup. They suggested more possible delivery vehicles to our project and a more logical poster
 +
                    design. We took part in a quiz competition and were awarded the prize for best collaboration quiz
 +
                    with our buddy team SFLS_Shenzhen. After the meetup, we're still talking on WeChat.
 +
                </p>
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/8/82/T--HZAU-China--OUYA1.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/a/ac/T--HZAU-China--OUYA2.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
                <div class="h1">CCiC meeting</div>
 +
                <p>In August, we participated in the biggest iGEMer communication conference in China——Conference of
 +
                    China iGEMer Community (CCiC). During this meeting, we communicated with teams tracking on therapy and reflected more about our project. For further collaboration，we met the captain of WHU-China and
 +
                    talked about the experience of our optogenetic tool.</p>
 +
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/e/e0/T--HZAU-China--CCiC1.jpg" width=100% alt="">
 +
                        </div>
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/4/47/T--HZAU-China--CCiC2.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/3/32/T--HZAU-China--CCiC3.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
 +
 +
 +
 +
  
 
             </div>
 
             </div>

Latest revision as of 20:37, 17 October 2018

Collaboration with WHU-China and SDU-CHINA

Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to use this system in their own projects. In the past two years, we have spent a great deal of time using it in our projects and accumulated a lot of experience (see more information in our previous wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in their projects and sharing the experience how we regulated the expression of CcaS and CcaR protein to control this optogenetic tool and what we achieved.

Experience sharing with Worldshaper-Wuhan

We cooperated with the high school team, Worldshaper-Wuhan. We shared some information about our project and had several discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines. LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not. Secondly, we want to explore its applicability for which this biomarker can be widely utilized to detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS has specificity and universality. There were significant expression differences among 293T (the healthy cell), 769-P (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P and 786-O were higher than that of 293T, showing specificity and universality of IL7-AS. Through our team's suggestions and Worldshaper-Wuhan's experiment, their project improved and we also got a new biomarker and therapeutic target for future projects.

Help from NAU-CHINA

After the information we got from meetings and public investigation, we thought it’s necessary to enhance the targeting of engineered Salmonella we used, we designed a fuse protein which called OmpA-RGD to achieve this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3 (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of Biochemistry and Cell Biology.

Collaboration with HUST-China

When we submitted our parts, we got the help of HUST-China. HUST-China helped us freeze the plasmid into dry powder in 96 well plate format, label our plate with our team name and submission number, and eventually send it by express to the agency in China. We are extremely grateful for their help.

EurAisan Meetup

We also took part in the iGEM EurAisan Meetup, where we shared our ideas and learned a lot. At the meeting, we had friendly communications with many iGEMers and gained a lot of advice during this meetup. They suggested more possible delivery vehicles to our project and a more logical poster design. We took part in a quiz competition and were awarded the prize for best collaboration quiz with our buddy team SFLS_Shenzhen. After the meetup, we're still talking on WeChat.

CCiC meeting

In August, we participated in the biggest iGEMer communication conference in China——Conference of China iGEMer Community (CCiC). During this meeting, we communicated with teams tracking on therapy and reflected more about our project. For further collaboration，we met the captain of WHU-China and talked about the experience of our optogenetic tool.

Team Attributions
Collaborations
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