Difference between revisions of "Team:HZAU-China/Collaborations"

 
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                     and SDU-CHINA</div>
 
                     and SDU-CHINA</div>
 
                 <p>Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is
 
                 <p>Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is
−
                     developed from <i>cyanobacteria</i> has made obvious achievements, thus more and more teams are willing to
+
                     developed from <i>cyanobacteria</i> has made obvious achievements, thus more and more teams are
 +
                    willing to
 
                     use this system in their own projects. In the past two years, we have spent a great deal of time
 
                     use this system in their own projects. In the past two years, we have spent a great deal of time
 
                     using it in our projects and accumulated a lot of experience (see more information in our previous
 
                     using it in our projects and accumulated a lot of experience (see more information in our previous
−
                     wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in project and
+
                     wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in their projects and
 
                     sharing the experience how we regulated the expression of CcaS and CcaR protein to control this
 
                     sharing the experience how we regulated the expression of CcaS and CcaR protein to control this
 
                     optogenetic tool and what we achieved.
 
                     optogenetic tool and what we achieved.
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                 <div class="row">
 
                     <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 
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                         <div style="width: 60%; margin: 30px auto">
 
                             <a href="https://2018.igem.org/Team:WHU-China/Collaborations"><img src="https://static.igem.org/mediawiki/2018/9/9a/T--WHU-China--wiki-Collabration_whu.png"
 
                             <a href="https://2018.igem.org/Team:WHU-China/Collaborations"><img src="https://static.igem.org/mediawiki/2018/9/9a/T--WHU-China--wiki-Collabration_whu.png"
 
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                                     width=100% alt=""></a>
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                     </div>
 
                     </div>
 
                     <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 
                     <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
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                         <div style="width: 80%; margin: 30px auto">
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                         <div style="width: 60%; margin: 30px auto">
 
                             <a href="https://2018.igem.org/Team:SDU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/c/c5/T--HZAU-China--SDU.png"
 
                             <a href="https://2018.igem.org/Team:SDU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/c/c5/T--HZAU-China--SDU.png"
 
                                     width=100% alt=""></a>
 
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             <div id="float02">
 
             <div id="float02">
 
                 <div class="h1">Experience sharing with Worldshaper-Wuhan</div>
 
                 <div class="h1">Experience sharing with Worldshaper-Wuhan</div>
−
                 <p>We cooperated with the high school team, Worldshaper-Wuhan. We shared some information about our project and had several
+
                 <p>We cooperated with the high school team, Worldshaper-Wuhan. We shared some information about our
 +
                    project and had several
 
                     discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines.
 
                     discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines.
 
                     LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and
 
                     LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and
 
                     therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if
 
                     therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if
 
                     the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not.
 
                     the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not.
−
                     Secondly, we want to expire its applicability for which this biomarker can be widely utilized to
+
                     Secondly, we want to explore its applicability for which this biomarker can be widely utilized to
−
                     detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS had speciality and
+
                     detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS has specificity and
 
                     universality. There were significant expression differences among 293T (the healthy cell), 769-P
 
                     universality. There were significant expression differences among 293T (the healthy cell), 769-P
 
                     (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P
 
                     (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P
−
                     and 786-O were higher than that of 293T, showing speciality and universality of IL7-AS. Through our
+
                     and 786-O were higher than that of 293T, showing specificity and universality of IL7-AS. Through our
−
                     team's suggestions and Worldshaper-Wuhan experiment, their project improved and we also had a new biomarker
+
                     team's suggestions and Worldshaper-Wuhan's experiment, their project improved and we also got a new
 +
                    biomarker
 
                     and therapeutic target for future projects.
 
                     and therapeutic target for future projects.
 
                 </p>
 
                 </p>
−
                 <div style="width: 30%; margin: 30px auto">
+
                 <div style="width: 20%; margin: 30px auto">
 
                     <a href="https://2018.igem.org/Team:Worldshaper-Wuhan/Collaborations"><img src="https://static.igem.org/mediawiki/2018/b/b6/T--HZAU-China--WSWH.png"
 
                     <a href="https://2018.igem.org/Team:Worldshaper-Wuhan/Collaborations"><img src="https://static.igem.org/mediawiki/2018/b/b6/T--HZAU-China--WSWH.png"
 
                             width=100% alt=""></a>
 
                             width=100% alt=""></a>
 
                 </div>
 
                 </div>
 +
 +
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                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/0/02/T--HZAU-China--WSWH1.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
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                    <div class="col-xs-6 col-sm-6 col-md-4 col-lg-4">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/9/97/T--HZAU-China--WSWH2.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
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                    <div class="col-xs-6 col-sm-6 col-md-4 col-lg-4">
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                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/3/35/T--HZAU-China--WSWH3.png" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
  
 
             </div>
 
             </div>
 
             <div id="float03">
 
             <div id="float03">
 
                 <div class="h1">Help from NAU-CHINA</div>
 
                 <div class="h1">Help from NAU-CHINA</div>
−
                 <p>After the information we got from meetings and public investigation, we thought it’s necessary to
+
 
−
                    enhance the targeting of engineered salmonella we used, we designed OmpA-RGD protein to achieve
+
                 <div class="row">
−
                    this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3
+
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
−
                    (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called
+
                        <div style="width: 100%; margin: 30px auto; padding: 0 20px;">
−
                    MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with
+
                            <p>After the information we got from meetings and public investigation, we thought it’s
−
                    the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of
+
                                necessary to
−
                    Biochemistry and Cell Biology.
+
                                enhance the targeting of engineered <i>Salmonella</i> we used, we designed a fuse
 +
                                protein which
 +
                                called OmpA-RGD to achieve
 +
                                this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to
 +
                                alpha v beta 3
 +
                                (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line
 +
                                called
 +
                                MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety
 +
                                experiments, with
 +
                                the great help of NAU-CHINA , we had channels to get this cell line from Shanghai
 +
                                Institute of
 +
                                Biochemistry and Cell Biology.
 +
                            </p>
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 80%; margin: 30px auto; ">
 +
                            <a href="https://2018.igem.org/Team:NAU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/8/88/T--HZAU-China--NAU.png"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 
 +
                <div class="h1">Collaboration with HUST-China</div>
 +
                <p>When we submitted our parts, we got the help of HUST-China. HUST-China
 +
                    helped us freeze the plasmid into dry powder in 96 well plate format, label our plate with our team name and
 +
                    submission number, and eventually send it by express to the agency in China. We are extremely grateful for their help.
 
                 </p>
 
                 </p>
−
                 <div style="width: 40%; margin: 30px auto">
+
 
−
                    <a href="https://2018.igem.org/Team:NAU-CHINA/Collaborations"><img src="https://static.igem.org/mediawiki/2018/8/88/T--HZAU-China--NAU.png"
+
                 <div class="row">
−
                            width=100% alt=""></a>
+
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; box-shadow: 0 1px 3px #676767; background-color: #333333; ">
 +
                            <a href="https://2018.igem.org/Team:HUST-China/Collaborations"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--HZAU-China--HUST1.jpg"
 +
                                    width=100% alt=""></a>
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/0/06/T--HZAU-China--HUST.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
 
 
                 </div>
 
                 </div>
 +
 +
                <div class="h1">EurAisan Meetup</div>
 +
                <p>We also took part in the iGEM EurAisan Meetup, where we shared our ideas and learned a lot. At the
 +
                    meeting, we had friendly communications with many iGEMers and gained a lot of advice during this
 +
                    meetup. They suggested more possible delivery vehicles to our project and a more logical poster
 +
                    design. We took part in a quiz competition and were awarded the prize for best collaboration quiz
 +
                    with our buddy team SFLS_Shenzhen. After the meetup, we're still talking on WeChat.
 +
                </p>
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/8/82/T--HZAU-China--OUYA1.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/a/ac/T--HZAU-China--OUYA2.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
                <div class="h1">CCiC meeting</div>
 +
                <p>In August, we participated in the biggest iGEMer communication conference in China——Conference of
 +
                    China iGEMer Community (CCiC). During this meeting, we communicated with teams tracking on therapy and reflected more about our project. For further collaboration，we met the captain of WHU-China and
 +
                    talked about the experience of our optogenetic tool.</p>
 +
 +
 +
                <div class="row">
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767; ">
 +
                            <img src="https://static.igem.org/mediawiki/2018/e/e0/T--HZAU-China--CCiC1.jpg" width=100% alt="">
 +
                        </div>
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/4/47/T--HZAU-China--CCiC2.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                    <div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
                        <div style="width: 100%; margin: 30px auto; border:8px solid #E0CCAE; box-shadow: 0 1px 3px #676767;">
 +
                            <img src="https://static.igem.org/mediawiki/2018/3/32/T--HZAU-China--CCiC3.jpg" width=100% alt="">
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 +
 +
 +
 +
 +
  
 
             </div>
 
             </div>

Latest revision as of 20:37, 17 October 2018

Collaboration with WHU-China and SDU-CHINA

Optogenetic tool has broad prospects on synthetic biology, and the CcaS-CcaR system, which is developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to use this system in their own projects. In the past two years, we have spent a great deal of time using it in our projects and accumulated a lot of experience (see more information in our previous wikis). This year, we collaborated with WHU-China and SDU-CHINA who use this system in their projects and sharing the experience how we regulated the expression of CcaS and CcaR protein to control this optogenetic tool and what we achieved.

Experience sharing with Worldshaper-Wuhan

We cooperated with the high school team, Worldshaper-Wuhan. We shared some information about our project and had several discussions then determined to have an experiment about the lncRNA IL7-AS in multiple cell lines. LncRNA IL7-AS is a newly discovered lncRNA, which might be a potential diagnostic biomarker and therapeutic target for renal cell carcinoma. Through this experiment, firstly, we want to know if the IL7-AS has tissue specificity to distinguish between cancer cells and healthy cells or not. Secondly, we want to explore its applicability for which this biomarker can be widely utilized to detect tumours. The Worldshaper-Wuhan team successfully demonstrated that IL7-AS has specificity and universality. There were significant expression differences among 293T (the healthy cell), 769-P (the adenocarcinoma cell) and 786-O (the adenocarcinoma cell) cell lines. The expression in 769-P and 786-O were higher than that of 293T, showing specificity and universality of IL7-AS. Through our team's suggestions and Worldshaper-Wuhan's experiment, their project improved and we also got a new biomarker and therapeutic target for future projects.

Help from NAU-CHINA

After the information we got from meetings and public investigation, we thought it’s necessary to enhance the targeting of engineered Salmonella we used, we designed a fuse protein which called OmpA-RGD to achieve this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3 (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of Biochemistry and Cell Biology.

Collaboration with HUST-China

When we submitted our parts, we got the help of HUST-China. HUST-China helped us freeze the plasmid into dry powder in 96 well plate format, label our plate with our team name and submission number, and eventually send it by express to the agency in China. We are extremely grateful for their help.

EurAisan Meetup

We also took part in the iGEM EurAisan Meetup, where we shared our ideas and learned a lot. At the meeting, we had friendly communications with many iGEMers and gained a lot of advice during this meetup. They suggested more possible delivery vehicles to our project and a more logical poster design. We took part in a quiz competition and were awarded the prize for best collaboration quiz with our buddy team SFLS_Shenzhen. After the meetup, we're still talking on WeChat.

CCiC meeting

In August, we participated in the biggest iGEMer communication conference in China——Conference of China iGEMer Community (CCiC). During this meeting, we communicated with teams tracking on therapy and reflected more about our project. For further collaboration，we met the captain of WHU-China and talked about the experience of our optogenetic tool.

Team Attributions
Collaborations
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