Difference between revisions of "Team:NU Kazakhstan/Project Timeline"

Revision as of 20:51, 17 October 2018

Bioremediation of Sour Crude Oil Waste using Cyanobacteria

May

Week 1-4

Week 1	April 30 - May 6
Week 2	May 7 - May 13
Week 3	May 14 - May 20
Week 4	May 21 - May 27

June

Week 5-9

Week 5	May 28 – June 3
Week 6	June 4 – June 10
Week 7	June 11 – June 17
Week 8	June 18 – June 24
Week 9	June 25 – July 1

WEEK 1 (April 30 - May 6)

5 May 2018
BG-11 medium was prepared.

Inoculation of the BG-11 medium with cyanobacteria (Synechococcus elongatus PCC 7942) was done.

WEEK 2 (May 7 - May 13)

11 May 2018
BG-11 agar plates were made.

Cyanobacteria were plated onto BG-11 agar plates from liquid culture

WEEK 3 (May 14 - May 20)

14 May 2018
The new subculture of cyanobacteria was prepared from old liquid culture.

The old liquid culture of cyanobacteria was plated onto BG -11 agar plate.
16 May 2018
BG-11 agar plates were prepared.

Cyanobacteria from liquid culture were plated onto the BG-11 agar plates.

WEEK 4 (May 21 - May 27)

25 May 2018
OD of liquid cyanobacteria culture was measured

Liquid cultures of cyanobacteria were checked.

The new subculture of cyanobacteria was prepared from old liquid culture.

WEEK 5 (May 28 – June 3)

28 May 2018
OD of liquid cyanobacteria culture was measured.

Liquid cultures of cyanobacteria were checked.
30 May 2018
LB liquid medium and LB agar plates were prepared.
31 May 2018
Competent E.coli (TOP10) cells were prepared.

WEEK 6 (June 4 – June 10)

4 June 2018
New competent TOP10 cells were prepared.

Spectinomycin stock solution 1000X was prepared.

LB agar plates with spectinomycin were prepared.
5 June 2018
Transformation of E.coli (TOP10) with empty pSyn_6 vector (marker gene: spectinomycin resistance gene) was done.

Transformed E.coli cells were plated on LB agar plates with spectinomycin.
6 June 2018
LB liquid medium with spectinomycin was prepared.

Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
7 June 2018
Plasmids from the transformed TOP10 were extracted by MiraPrep.

Extracted plasmid samples were measured by Nanodrop.

Samples 260/280 260/230 Concentration

1 1.76 1.53 63.96 ng/ul

2 1.96 2.29 1953 ng/u

Gel Electrophoresis was done.
8 June 2018
OD of liquid cyanobacteria culture was measured. OD: 0.8.

BG-11 agar plates with spectinomycin were prepared.

WEEK 7 (June 11 – June 17)

11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
12 June 2018
MiraPrep was done.

Extracted plasmid samples were measured by Nanodrop.

Samples 260/280 260/230 Concentration

1 1.90 2.38 1600 ng/ul

Gel Electrophoresis was done.

Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.
11 June 2018
Inoculation of the LB liquid medium with spectinomycin resistant TOP10 cells was done.

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

WEEK 5

25 May 2018
OD of liquid cyanobacteria culture was measured

@@ Line 498: / Line 498: @@
 					<h6 class="text-uppercase mb-20">Quick About</h6>
 					<p>
-						SCHOOL OF SCIENCE AND TECHNOLOGY Nazarbayev University Astana, Kazakhstan
+						SCHOOL OF SCIENCE AND TECHNOLOGY <br> Nazarbayev University <br> Astana, Kazakhstan
 					</p>
 				</div>

Samples	260/280	260/230	Concentration
1	1.76	1.53	63.96 ng/ul
2	1.96	2.29	1953 ng/u