Difference between revisions of "Team:RHIT/Results"

 
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<h1>Results</h1>
 
<h1>Results</h1>
<p>Here you can describe the results of your project and your future plans. </p>
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<p> Below are the results of the SDS page we performed on our Glcyolate Oxidase gene. </p>
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The Glycolate Oxidase we submitted was cloned from /E. coli/ MG1655 and contains three subunits. It was inserted into the pETDuet vector for expression and was induced by 0.1 and 0.2 mM IPTG at 30ºC for 20 hours. All three subunits of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDuet vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were induced with 0.1 mM IPTG, and lanes 5 and 7 were induced with 0.2 mM IPTG. All the inductions were done at 30ºC.</p>
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<h3>What should this page contain?</h3>
 
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<li> Clearly and objectively describe the results of your work.</li>
 
<li> Future plans for the project. </li>
 
<li> Considerations for replicating the experiments. </li>
 
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<h3>Describe what your results mean </h3>
 
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
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<h3>Inspiration</h3>
 
<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 21:04, 17 October 2018




Results

Below are the results of the SDS page we performed on our Glcyolate Oxidase gene.

The Glycolate Oxidase we submitted was cloned from /E. coli/ MG1655 and contains three subunits. It was inserted into the pETDuet vector for expression and was induced by 0.1 and 0.2 mM IPTG at 30ºC for 20 hours. All three subunits of Glycolate Oxidase(GO) are shown in the SDS-PAGE. GO contains three subunits, D,E, and F, which weigh 55.5 kDa, 38.3 kDa, and 45.0 kDa respectively. Lane 1 was BL21(DE3) with pETDuet-GO, but without IPTG induction process. Lanes 2 and 3 were BL21(DE3) with empty pETDuet vectors and contain 0.1 and 0.2 mM IPTG. Lanes 4 to 7 are BL21(De3) with pETDuet-GO. Lanes 4 and 6 were induced with 0.1 mM IPTG, and lanes 5 and 7 were induced with 0.2 mM IPTG. All the inductions were done at 30ºC.