Difference between revisions of "Team:Oxford/protocols"

 
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                    <h1 class="header-text2">Protocols</h1>
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<h1>Protocols <br><br></h1>
 
  
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<p> We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project. </p>
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        <a data-toggle="collapse" href="#culture">Cell Culturing</a>
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/31/T--Oxford--_aculture.pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#competent">Competent Cells</a>
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    <div id="competent" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/9/9b/T--Oxford--a_Cells_competency.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#colony">Colony PCR</a>
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/d/d0/T--Oxford--a_Colony.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and primers</a>
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    <div id="dilution" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e5/T--Oxford--a_Dilution.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#digests">DNA Digests</a>
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/6e/T--Oxford--a_Digest.pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#ligation">DNA Ligation</a>
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/51/T--Oxford--a_ligations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#transformation">DNA Transformation</a>
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/62/T--Oxford--_a_transformations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#analysis">Gel Analysis</a>
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        <a data-toggle="collapse" href="#extraction">Gel Extraction</a>
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        <a data-toggle="collapse" href="#loading">Gel Loading</a>
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        <a data-toggle="collapse" href="#prep">Gel Preparation</a>
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        <a data-toggle="collapse" href="#oePCR">oePCR</a>
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        <a data-toggle="collapse" href="#standard">Standard PCR</a>
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Latest revision as of 21:55, 17 October 2018

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Protocols

We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.

Cell Culturing

Competent Cells

Colony PCR

Dilution of g-Blocks and primers

DNA Digests

DNA Ligation

DNA Transformation

Gel Analysis

Gel Extraction

Gel Loading

Gel Preparation

oePCR

Standard PCR