Difference between revisions of "Team:Oxford/protocols"

 
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                    <h1 class="header-text2">Protocols</h1>
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<h2>Protocols <br><br></h2>
 
  
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<p> We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project. </p>
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#culture">Cell Culturing</a>
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      </h4>
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    </div>
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    <div id="culture" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/31/T--Oxford--_aculture.pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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</div>
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<div class="panel-group">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#competent">Competent Cells</a>
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      </h4>
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    <div id="competent" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/9/9b/T--Oxford--a_Cells_competency.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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<div class="panel-group">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#colony">Colony PCR</a>
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      </h4>
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    </div>
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    <div id="colony" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/d/d0/T--Oxford--a_Colony.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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</div>
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<div class="panel-group">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and primers</a>
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      </h4>
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    </div>
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    <div id="dilution" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e5/T--Oxford--a_Dilution.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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</div>
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<div class="panel-group">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#digests">DNA Digests</a>
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      </h4>
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    </div>
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    <div id="digests" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/6e/T--Oxford--a_Digest.pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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</div>
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<div class="panel-group">
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  <div class="panel panel-default">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#ligation">DNA Ligation</a>
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      </h4>
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    </div>
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    <div id="ligation" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/51/T--Oxford--a_ligations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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<div class="panel-group">
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    <div class="panel-heading">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#transformation">DNA Transformation</a>
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      </h4>
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    </div>
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    <div id="transformation" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/62/T--Oxford--_a_transformations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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<div class="panel-group">
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#analysis">Gel Analysis</a>
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      </h4>
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    </div>
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    <div id="analysis" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e7/T--Oxford--_a_analysis.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#extraction">Gel Extraction</a>
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      </h4>
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    <div id="extraction" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/1/13/T--Oxford--_a_gel_extraction.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#loading">Gel Loading</a>
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      </h4>
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    <div id="loading" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/33/T--Oxford--_a_gel_loading.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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        <a data-toggle="collapse" href="#prep">Gel Preparation</a>
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    <div id="prep" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/8/89/T--Oxford--_agelprep.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#oePCR">oePCR</a>
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      </h4>
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    </div>
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    <div id="oePCR" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/55/T--Oxford--_aoePCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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      <h4 class="panel-title">
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        <a data-toggle="collapse" href="#standard">Standard PCR</a>
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      </h4>
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    </div>
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    <div id="standard" class="panel-collapse collapse">
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      <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/b/b2/T--Oxford--a_Standard_PCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center>
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          <img class="rounded-circle img-fluid d-block mx-auto" src="http://placehold.it/200x200" alt="">
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          <div class="protocol-header">Competent cells</div>
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          <div class="protocol-header">Gel analysis and extraction</div>
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          <img class="rounded-circle img-fluid d-block mx-auto" src="http://placehold.it/200x200" alt="">
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          <div class="protocol-header">Transformation</div>
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Latest revision as of 21:55, 17 October 2018

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Protocols

We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.

Cell Culturing

Competent Cells

Colony PCR

Dilution of g-Blocks and primers

DNA Digests

DNA Ligation

DNA Transformation

Gel Analysis

Gel Extraction

Gel Loading

Gel Preparation

oePCR

Standard PCR