Difference between revisions of "Team:Oxford/protocols"
| (29 intermediate revisions by 3 users not shown) | |||
| Line 18: | Line 18: | ||
<link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.2.0/css/all.css" integrity="sha384-hWVjflwFxL6sNzntih27bfxkr27PmbbK/iSvJ+a4+0owXq79v+lsFkW54bOGbiDQ" crossorigin="anonymous"> | <link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.2.0/css/all.css" integrity="sha384-hWVjflwFxL6sNzntih27bfxkr27PmbbK/iSvJ+a4+0owXq79v+lsFkW54bOGbiDQ" crossorigin="anonymous"> | ||
| − | < | + | </head> |
| − | + | <body> | |
| − | + | <header class="py-5 background-img parent" style="height: 400px;"> | |
| − | <h1 class="header- | + | <a href="#n1"style="text-decoration : none; color:white;"> |
| − | </div> | + | <div class="child background-img" style="background-image: url('https://static.igem.org/mediawiki/2018/a/af/T--Oxford--lab8.jpg'); background-size: cover;"> |
| + | <div class="translucent-back" style="height: 400px;"> | ||
| + | <h1 class="header-text2">Protocols</h1> | ||
| + | </div> | ||
| + | </div> | ||
| + | </a> | ||
</header> | </header> | ||
| − | |||
| − | + | ||
| Line 35: | Line 39: | ||
<div class="row"> | <div class="row"> | ||
<div class=".col-md-8 .offset-md-2"> | <div class=".col-md-8 .offset-md-2"> | ||
| − | |||
<p> We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project. </p> | <p> We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project. </p> | ||
| Line 44: | Line 47: | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#culture">Cell Culturing</a> | <a data-toggle="collapse" href="#culture">Cell Culturing</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="culture" class="panel-collapse collapse"> | <div id="culture" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/31/T--Oxford--_aculture.pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
| Line 57: | Line 61: | ||
<a data-toggle="collapse" href="#competent">Competent Cells</a> | <a data-toggle="collapse" href="#competent">Competent Cells</a> | ||
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="competent" class="panel-collapse collapse"> | <div id="competent" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/9/9b/T--Oxford--a_Cells_competency.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
| Line 69: | Line 74: | ||
<a data-toggle="collapse" href="#colony">Colony PCR</a> | <a data-toggle="collapse" href="#colony">Colony PCR</a> | ||
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="colony" class="panel-collapse collapse"> | <div id="colony" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/d/d0/T--Oxford--a_Colony.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and | + | <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and primers</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="dilution" class="panel-collapse collapse"> | <div id="dilution" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e5/T--Oxford--a_Dilution.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#digests">DNA Digests</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
| − | <div id=" | + | <div id="digests" class="panel-collapse collapse"> |
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/6e/T--Oxford--a_Digest.pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#ligation">DNA | + | <a data-toggle="collapse" href="#ligation">DNA Ligation</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="ligation" class="panel-collapse collapse"> | <div id="ligation" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/51/T--Oxford--a_ligations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#transformation">DNA Transformation</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
| − | <div id=" | + | <div id="transformation" class="panel-collapse collapse"> |
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/62/T--Oxford--_a_transformations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#analysis">Gel Analysis</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
| − | <div id=" | + | <div id="analysis" class="panel-collapse collapse"> |
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e7/T--Oxford--_a_analysis.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#extraction">Gel | + | <a data-toggle="collapse" href="#extraction">Gel Extraction</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="extraction" class="panel-collapse collapse"> | <div id="extraction" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/1/13/T--Oxford--_a_gel_extraction.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
| + | |||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
| Line 153: | Line 167: | ||
<a data-toggle="collapse" href="#loading">Gel Loading</a> | <a data-toggle="collapse" href="#loading">Gel Loading</a> | ||
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="loading" class="panel-collapse collapse"> | <div id="loading" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/33/T--Oxford--_a_gel_loading.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
| Line 165: | Line 181: | ||
<a data-toggle="collapse" href="#prep">Gel Preparation</a> | <a data-toggle="collapse" href="#prep">Gel Preparation</a> | ||
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="prep" class="panel-collapse collapse"> | <div id="prep" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/8/89/T--Oxford--_agelprep.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#oePCR">oePCR</a> |
</h4> | </h4> | ||
| − | </div | + | </div> |
| − | <div id=" | + | <div id="oePCR" class="panel-collapse collapse"> |
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/55/T--Oxford--_aoePCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
| + | |||
<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
| Line 189: | Line 208: | ||
<a data-toggle="collapse" href="#standard">Standard PCR</a> | <a data-toggle="collapse" href="#standard">Standard PCR</a> | ||
</h4> | </h4> | ||
| − | </div | + | </div> |
<div id="standard" class="panel-collapse collapse"> | <div id="standard" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/b/b2/T--Oxford--a_Standard_PCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| Line 220: | Line 243: | ||
<!-- Bootstrap core JavaScript --> | <!-- Bootstrap core JavaScript --> | ||
<script src="vendor/jquery/jquery.min.js"></script> | <script src="vendor/jquery/jquery.min.js"></script> | ||
| − | <script src=" | + | <script src="https://2018.igem.org/Team:Oxford/scripts/bootstrapminjs?action=raw&ctype=text/javascript"></script> |
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 21:55, 17 October 2018
Protocols
We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.