Difference between revisions of "Team:Oxford/protocols"
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<link href="https://2018.igem.org/Team:Oxford/css?action=raw&ctype=text/css" rel="stylesheet"> | <link href="https://2018.igem.org/Team:Oxford/css?action=raw&ctype=text/css" rel="stylesheet"> | ||
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<body> | <body> | ||
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</header> | </header> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#culture">Cell Culturing</a> | <a data-toggle="collapse" href="#culture">Cell Culturing</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="culture" class="panel-collapse collapse"> | <div id="culture" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/31/T--Oxford--_aculture.pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#competent">Competent Cells</a> | <a data-toggle="collapse" href="#competent">Competent Cells</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="competent" class="panel-collapse collapse"> | <div id="competent" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/9/9b/T--Oxford--a_Cells_competency.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#colony">Colony PCR</a> | <a data-toggle="collapse" href="#colony">Colony PCR</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="colony" class="panel-collapse collapse"> | <div id="colony" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/d/d0/T--Oxford--a_Colony.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and | + | <a data-toggle="collapse" href="#dilution">Dilution of g-Blocks and primers</a> |
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="dilution" class="panel-collapse collapse"> | <div id="dilution" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e5/T--Oxford--a_Dilution.pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#digests">DNA Digests</a> |
| − | </h4> <object data="https://static.igem.org/mediawiki/2018/6/6e/T--Oxford--a_Digest.pdf" type="application/pdf" width="100%" height="600px"></object> | + | </h4> |
| − | + | </div> | |
| − | + | <div id="digests" class="panel-collapse collapse"> | |
| − | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/6e/T--Oxford--a_Digest.pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#ligation">DNA | + | <a data-toggle="collapse" href="#ligation">DNA Ligation</a> |
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="ligation" class="panel-collapse collapse"> | <div id="ligation" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/51/T--Oxford--a_ligations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#transformation">DNA Transformation</a> |
| − | </h4><object data="https://static.igem.org/mediawiki/2018/6/62/T--Oxford--_a_transformations.pdf" type="application/pdf" width="100%" height="600px"></object> | + | </h4> |
| − | + | </div> | |
| − | + | <div id="transformation" class="panel-collapse collapse"> | |
| − | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/6/62/T--Oxford--_a_transformations.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#analysis">Gel Analysis</a> |
| − | </h4><object data= "https://static.igem.org/mediawiki/2018/e/e7/T--Oxford--_a_analysis.pdf" type="application/pdf" width="100%" height="600px"></object> | + | </h4> |
| − | + | </div> | |
| − | + | <div id="analysis" class="panel-collapse collapse"> | |
| − | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/e/e7/T--Oxford--_a_analysis.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="#extraction">Gel | + | <a data-toggle="collapse" href="#extraction">Gel Extraction</a> |
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="extraction" class="panel-collapse collapse"> | <div id="extraction" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/1/13/T--Oxford--_a_gel_extraction.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#loading">Gel Loading</a> | <a data-toggle="collapse" href="#loading">Gel Loading</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="loading" class="panel-collapse collapse"> | <div id="loading" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/3/33/T--Oxford--_a_gel_loading.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#prep">Gel Preparation</a> | <a data-toggle="collapse" href="#prep">Gel Preparation</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="prep" class="panel-collapse collapse"> | <div id="prep" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/8/89/T--Oxford--_agelprep.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
<div class="panel-heading"> | <div class="panel-heading"> | ||
<h4 class="panel-title"> | <h4 class="panel-title"> | ||
| − | <a data-toggle="collapse" href="# | + | <a data-toggle="collapse" href="#oePCR">oePCR</a> |
| − | </h4><object data="https://static.igem.org/mediawiki/2018/ | + | </h4> |
| − | + | </div> | |
| − | + | <div id="oePCR" class="panel-collapse collapse"> | |
| − | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/5/55/T--Oxford--_aoePCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="panel-group"> | <div class="panel-group"> | ||
<div class="panel panel-default"> | <div class="panel panel-default"> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" href="#standard">Standard PCR</a> | <a data-toggle="collapse" href="#standard">Standard PCR</a> | ||
| − | </h4 | + | </h4> |
| − | </div | + | </div> |
<div id="standard" class="panel-collapse collapse"> | <div id="standard" class="panel-collapse collapse"> | ||
| − | <div class="panel-body">< | + | <center><div class="panel-body"><object data="https://static.igem.org/mediawiki/2018/b/b2/T--Oxford--a_Standard_PCR.pdf" type="application/pdf" type="application/pdf" type="application/pdf" type="application/pdf" width="100%" height="600px"></object></div></center> |
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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<!-- Bootstrap core JavaScript --> | <!-- Bootstrap core JavaScript --> | ||
<script src="vendor/jquery/jquery.min.js"></script> | <script src="vendor/jquery/jquery.min.js"></script> | ||
| − | <script src=" | + | <script src="https://2018.igem.org/Team:Oxford/scripts/bootstrapminjs?action=raw&ctype=text/javascript"></script> |
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 21:55, 17 October 2018
Protocols
We used the website Benchling to write down all of our protocols to make sure they were easily accessible to all members of the team. Below are some of the most commonly used protocols over this project.