Difference between revisions of "Team:Munich/chibiobrick1.html"
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| − | <h4>Forming | + | <h4>Forming Oligodimers from Single-Strand DNA chi6 Sequences</h4> |
<em>2018/06/01</em> | <em>2018/06/01</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 9: | Line 9: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>Oligodimerization</td> | + | <td><a href="https://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html" target="_blank">Oligodimerization</a> |
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td> Oligodimers were bought | ||
| + | </td> | ||
| + | </tr> | ||
| + | |||
</tr> | </tr> | ||
</table> | </table> | ||
| Line 24: | Line 32: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Protocol:</td> |
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank">Restriction digest</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Miniprep</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/ab/T--Munich--WL1_Sequencing_sample_preparation.pdf" target="_blank">Sequencing</a> | ||
| + | |||
| + | |||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>pSB1C3 Backbone was generated by restriction with XbaI and SpeI. Chi6 Dimers were digested with XbaI & SpeI.<br> |
| − | + | Transformation of Dh5a | |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>No sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 49: | Line 65: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Munich--WL1_Restriction_Digest.pdf" target="_blank">Restriction digest</a>, | ||
| + | <a href="https://international.neb.com/products/t1030-monarch-pcr-dna-cleanup-kit-5-ug#Product%20Information" target="_blank">PCR purification</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical Transformation</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td> Digest with XbaI, SpeI <br> |
| − | + | Transformation of Dh5a | |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td>No bands on the Gel. Colonies on the plate resulted from contamination.</td> | + | <td>No bands on the Gel. Colonies on the plate resulted from contamination (religation of backbound).</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 23:57, 17 October 2018
Forming Oligodimers from Single-Strand DNA chi6 Sequences
2018/06/01| Participants: | Dominic Schwarz |
| Protocol: | Oligodimerization |
| Notes: | Oligodimers were bought |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, Ligation, Chemical transformation, Miniprep, Sequencing |
| Notes: | pSB1C3 Backbone was generated by restriction with XbaI and SpeI. Chi6 Dimers were digested with XbaI & SpeI. Transformation of Dh5a |
| Results: | No sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | Digest with XbaI, SpeI Transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination (religation of backbound). |