Difference between revisions of "Team:Munich/chiversions.html"

 
(20 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 +
<html>
 
<div class="event-info">
 
<div class="event-info">
  
−
<h3>6/1</h3>
+
 
−
<h4>Forming oligodimers from single-strand DNA chi6 sequences</h4>
+
 
 +
<h4>PCR Amplify Linear mtq2</h4>
 +
<em>2018/05/28 - 2018/05/29</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
  
 
       <td>Participants:</td>
 
       <td>Participants:</td>
−
       <td>Dominic</td>
+
       <td>Dominic Schwarz</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>Oligodimerization</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td>
+
       <td>VR & VF2; TA: 66° ET: 50s; <br>
 +
Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor)
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td></td>
+
       <td>Worked (PIC)</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
  
−
<h3>6/4</h3>
+
<h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly</h4>
−
<h4>PCR to assemble Chi6(double) and Chi6-Mtq2-Chi6</h4>
+
<em>2018/06/04 - 2018/06/08</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
  
 
       <td>Participants:</td>
 
       <td>Participants:</td>
−
       <td>Dominic</td>
+
       <td>Dominic Schwarz</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel purification</a>,
 +
<a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td>
+
       <td>Fragments were amplified, then purified and Gibson Assembly was performed.
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td>no bands on gel</td>
+
       <td>No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
−
<h3>6/8</h3>
+
 
−
<h4>Gibson assembly of Chi6(double) and Chi6-Mtq2-Chi6</h4>
+
<h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR</h4>
 +
<em>2018/06/11 - 2018/06/14</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
  
 
       <td>Participants:</td>
 
       <td>Participants:</td>
−
       <td>Eni</td>
+
       <td>Dominic Schwarz</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>Gibson assembly</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td>M(100bp) / / 2x / flank / BBP-lacO / Term-BBS
+
       <td>Fragments were amplified, then purified and overlap PCR was performed.
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td>no bands on gel</td>
+
       <td>The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
 +
 +
<h4>PCR to Amplify Mtq2</h4>
 +
<em>2018/06/12</em>
 +
<table class="table table-borderless">
 +
    <tr>
 +
 +
      <td>Participants:</td>
 +
      <td>Dominic Schwarz</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Protocol:</td>
 +
      <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>
 +
</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Notes:</td>
 +
      <td> Primers: VF2, VR
 +
</td>
 +
    </tr>
 +
<tr>
 +
      <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
 +
      <td>No bands visible: redo.
 +
 +
<figure class="figure">
 +
  <img src="https://static.igem.org/mediawiki/2018/3/3f/T--munich--IHATEIGEMHQLINK120180529_mtq-VR-VF2_pKD3-KO.jpg " class="figure-img img-fluid rounded" alt=" ">
 +
    <figcaption class="figure-caption">PCR amplify linear mTQ2</figcaption>
 +
    </figure>
 +
 +
<figure class="figure">
 +
  <img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" ">
 +
    <figcaption class="figure-caption">We use 2 Log ladder from NEB as the marker</figcaption>
 +
    </figure>
 +
 +
</td>
 +
    </tr>
 +
</table>
  
 
</div>
 
</div>
 +
</html>

Latest revision as of 23:58, 17 October 2018

PCR Amplify Linear mtq2

2018/05/28 - 2018/05/29
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel Purification
Notes: VR & VF2; TA: 66° ET: 50s;
Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor)
Results: Worked (PIC)

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly

2018/06/04 - 2018/06/08
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel purification, Gibson Assembly
Notes: Fragments were amplified, then purified and Gibson Assembly was performed.
Results: No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR

2018/06/11 - 2018/06/14
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel extraction
Notes: Fragments were amplified, then purified and overlap PCR was performed.
Results: The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.

PCR to Amplify Mtq2

2018/06/12
Participants: Dominic Schwarz
Protocol: PCR
Notes: Primers: VF2, VR
Results: No bands visible: redo.
PCR amplify linear mTQ2
We use 2 Log ladder from NEB as the marker