Difference between revisions of "Team:Munich/chiversions.html"
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<div class="event-info"> | <div class="event-info"> | ||
| − | < | + | |
| − | < | + | |
| + | <h4>PCR Amplify Linear mtq2</h4> | ||
| + | <em>2018/05/28 - 2018/05/29</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>VR & VF2; TA: 66° ET: 50s; <br> |
| + | Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) | ||
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td></td> | + | <td>Worked (PIC)</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly</h4> | |
| − | <h4> | + | <em>2018/06/04 - 2018/06/08</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR</td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel purification</a>, | ||
| + | <a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>Fragments were amplified, then purified and Gibson Assembly was performed. |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | ||
| − | <h4> | + | <h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR</h4> |
| + | <em>2018/06/11 - 2018/06/14</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>Fragments were amplified, then purified and overlap PCR was performed. |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | < | + | <h4>PCR to Amplify Mtq2</h4> |
| − | < | + | <em>2018/06/12</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR</td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td> Primers: VF2, VR |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</ | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | + | <td>No bands visible: redo. | |
| − | + | ||
| − | + | ||
| + | <figure class="figure"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--munich--IHATEIGEMHQLINK120180529_mtq-VR-VF2_pKD3-KO.jpg " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption">PCR amplify linear mTQ2</figcaption> | ||
| + | </figure> | ||
| − | < | + | <figure class="figure"> |
| − | < | + | <img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" "> |
| − | < | + | <figcaption class="figure-caption">We use 2 Log ladder from NEB as the marker</figcaption> |
| − | < | + | </figure> |
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</td> | </td> | ||
| − | |||
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</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | </html> | ||
Latest revision as of 23:58, 17 October 2018
PCR Amplify Linear mtq2
2018/05/28 - 2018/05/29| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel Purification |
| Notes: | VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) |
| Results: | Worked (PIC) |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly
2018/06/04 - 2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel purification, Gibson Assembly |
| Notes: | Fragments were amplified, then purified and Gibson Assembly was performed. |
| Results: | No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly. |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR
2018/06/11 - 2018/06/14| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Fragments were amplified, then purified and overlap PCR was performed. |
| Results: | The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples. |
PCR to Amplify Mtq2
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | PCR |
| Notes: | Primers: VF2, VR |
| Results: | No bands visible: redo.
|