Difference between revisions of "Team:Munich/chiversions.html"

 
(10 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
  
−
<h4>PCR amplify linear mtq2</h4>
+
<h4>PCR Amplify Linear mtq2</h4>
 
<em>2018/05/28 - 2018/05/29</em>
 
<em>2018/05/28 - 2018/05/29</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
Line 14: Line 14:
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR, agarose gel, gel purification</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,  
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,  
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
Line 23: Line 27:
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td>worked (PIC)</td>
+
       <td>Worked (PIC)</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
  
−
<h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by gibson assembly</h4>
+
<h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly</h4>
 
<em>2018/06/04 - 2018/06/08</em>
 
<em>2018/06/04 - 2018/06/08</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
Line 39: Line 43:
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR, Agarose gel, gel purification, gibson assembly</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,  
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,  
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel purification</a>,  
 +
<a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td>Fragments were amplified, then purified and gibson assembly was performed  
+
       <td>Fragments were amplified, then purified and Gibson Assembly was performed.
 
</td>
 
</td>
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td>No bands were visible on the gel after gibson assembly, suggesting that fragments were not amplified correctly before. additionally, we decided to do overlap pcr instead of gibson assembly.</td>
+
       <td>No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
Line 63: Line 72:
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR, agarose gel, gel extraction</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,  
 +
<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,  
 +
<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
Line 71: Line 84:
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
 
       <td>The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.</td>
 
       <td>The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.</td>
 
     </tr>
 
     </tr>
Line 77: Line 90:
  
  
−
<h4>PCR to amplify Mtq2</h4>
+
<h4>PCR to Amplify Mtq2</h4>
 
<em>2018/06/12</em>
 
<em>2018/06/12</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
Line 87: Line 100:
 
     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
−
       <td>PCR</td>
+
       <td>
 +
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>
 +
</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
Line 95: Line 110:
 
     </tr>
 
     </tr>
 
  <tr>
 
  <tr>
−
       <td>Results:</td>
+
       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
−
       <td></td>
+
       <td>No bands visible: redo.
 +
 
 +
<figure class="figure">
 +
  <img src="https://static.igem.org/mediawiki/2018/3/3f/T--munich--IHATEIGEMHQLINK120180529_mtq-VR-VF2_pKD3-KO.jpg " class="figure-img img-fluid rounded" alt=" ">
 +
    <figcaption class="figure-caption">PCR amplify linear mTQ2</figcaption>
 +
    </figure>
 +
 
 +
<figure class="figure">
 +
  <img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" ">
 +
    <figcaption class="figure-caption">We use 2 Log ladder from NEB as the marker</figcaption>
 +
    </figure>
 +
 
 +
</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>

Latest revision as of 23:58, 17 October 2018

PCR Amplify Linear mtq2

2018/05/28 - 2018/05/29
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel Purification
Notes: VR & VF2; TA: 66° ET: 50s;
Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor)
Results: Worked (PIC)

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly

2018/06/04 - 2018/06/08
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel purification, Gibson Assembly
Notes: Fragments were amplified, then purified and Gibson Assembly was performed.
Results: No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR

2018/06/11 - 2018/06/14
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel extraction
Notes: Fragments were amplified, then purified and overlap PCR was performed.
Results: The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.

PCR to Amplify Mtq2

2018/06/12
Participants: Dominic Schwarz
Protocol: PCR
Notes: Primers: VF2, VR
Results: No bands visible: redo.
PCR amplify linear mTQ2
We use 2 Log ladder from NEB as the marker