Difference between revisions of "Team:Munich/chiversions.html"
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| − | <h4>PCR | + | <h4>PCR Amplify Linear mtq2</h4> |
<em>2018/05/28 - 2018/05/29</em> | <em>2018/05/28 - 2018/05/29</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 28: | Line 28: | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td> | + | <td>Worked (PIC)</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | <h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by | + | <h4>Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly</h4> |
<em>2018/06/04 - 2018/06/08</em> | <em>2018/06/04 - 2018/06/08</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 52: | Line 52: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td>Fragments were amplified, then purified and | + | <td>Fragments were amplified, then purified and Gibson Assembly was performed. |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td>No bands were visible on the gel after | + | <td>No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 90: | Line 90: | ||
| − | <h4>PCR to | + | <h4>PCR to Amplify Mtq2</h4> |
<em>2018/06/12</em> | <em>2018/06/12</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 111: | Line 111: | ||
<tr> | <tr> | ||
<td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> | ||
| − | <td></td> | + | <td>No bands visible: redo. |
| + | |||
| + | <figure class="figure"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--munich--IHATEIGEMHQLINK120180529_mtq-VR-VF2_pKD3-KO.jpg " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption">PCR amplify linear mTQ2</figcaption> | ||
| + | </figure> | ||
| + | |||
| + | <figure class="figure"> | ||
| + | <img src=https://www.neb.com/products/n3200-1-kb-plus-dna-ladder#Product%20Information " class="figure-img img-fluid rounded" alt=" "> | ||
| + | <figcaption class="figure-caption">We use 2 Log ladder from NEB as the marker</figcaption> | ||
| + | </figure> | ||
| + | |||
| + | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 23:58, 17 October 2018
PCR Amplify Linear mtq2
2018/05/28 - 2018/05/29| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel Purification |
| Notes: | VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) |
| Results: | Worked (PIC) |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by Gibson Assembly
2018/06/04 - 2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel purification, Gibson Assembly |
| Notes: | Fragments were amplified, then purified and Gibson Assembly was performed. |
| Results: | No bands were visible on the gel after Gibson Assembly, suggesting that fragments were not amplified correctly before. Additionally, we decided to do overlap PCR instead of Gibson Assembly. |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR
2018/06/11 - 2018/06/14| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Fragments were amplified, then purified and overlap PCR was performed. |
| Results: | The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples. |
PCR to Amplify Mtq2
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | PCR |
| Notes: | Primers: VF2, VR |
| Results: | No bands visible: redo.
|