Difference between revisions of "Team:SSHS-Shenzhen/Experiments"

 
(42 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{SSHS-Shenzhen/CSS}} <html lang="en"> <head>
 
{{SSHS-Shenzhen/CSS}} <html lang="en"> <head>
−
<meta charset="UTF-8"> <title>Title</title><style>
+
<meta charset="UTF-8"> <title>Title</title>
−
h1{
+
<style>
−
color: #fff;
+
.header li a:hover,.dropdown:hover.dropbtn {
 +
color: #fff;
 +
}
 +
.header {
 +
font-size: 30px;
 +
padding: 0px;
 +
margin: 0px;
 +
top: 0;
 +
left: 0;
 +
width: 100%;
 +
position: fixed;
 +
height: 3em;
 +
z-index: 99;
 +
float: left;
 +
background-color: #5d8aa8;
 +
min-width: 1080px;
 +
}
 +
body {
 +
background-color: #fff;
 +
}
 +
.dropdown-content {
 +
display: none;
 +
position: absolute;
 +
background-color: #5d8aa8;
 +
min-width: 250px
 +
}
 +
.dropdown-content a {
 +
color: #f7f7f7;
 +
padding: 12px 16px;
 +
text-decoration: none;
 +
display: block;
 +
font-size: 20px;
 +
}
 +
.header li a,.dropbtn,.header a:link, .header a:visited {
 +
color: #f7f7f7;
 +
}
 +
  #para{
 +
color: #000;
 +
padding:30px 100px 5px;
 +
font-size: 20px!important;
 +
}
 +
#fig{
 +
color: #000;
 +
padding:30px 100px 5px;
 +
font-size: 20px!important;
 +
text-align: center !important;
 +
}
 +
 +
h1{
 +
color: #000;
 
padding:150px 50px 5px!important; font-size: 30px!important; text-align: center;
 
padding:150px 50px 5px!important; font-size: 30px!important; text-align: center;
 
}
 
}
−
</style> </head>
+
 
 +
h2{
 +
color: #000;
 +
padding:100px 100px 5px;
 +
font-size: 25px!important;
 +
text-align: left;
 +
line-height: 120%;
 +
}
 +
 
 +
h3{
 +
color: #000;
 +
padding:100px 100px 5px;
 +
font-size: 25px!important;
 +
text-align: center;
 +
line-height: 120%;
 +
}
 +
 
 +
.banner2{
 +
color:#fff;
 +
background-color:#5d8aa8;
 +
font-size:100px!important;
 +
width:100%; height:500px;
 +
text-align:center;
 +
line-height: 500px;
 +
padding:90px 0px 0px;
 +
background-image:url("https://static.igem.org/mediawiki/2018/c/c1/T--SSHS-Shenzhen--test1.jpeg");
 +
}
 +
</style></head>
 
<body>
 
<body>
−
<div class="banner1">
+
<div class="banner2">
 
Experiments
 
Experiments
 
</div>
 
</div>
 +
<h1>
 +
Experimental SSHS-Shenzhen
 +
</h1>
  
 
<h1>
 
<h1>
 
Experimental summary
 
Experimental summary
 
</h1>
 
</h1>
−
<p id="para"> Short interfering RNAs (siRNAs) and their corresponding shRNAs were designed based on the mRNA sequences of their target genes. siRNAs were synthesized by a bio-company, shRNAs were produced by in vitro transcription. The efficiency of both siRNAs and shRNAs in mediating RNAi in Phyllotreta striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Results also show that GC percentage of the total siRNA/shRNA, the 5’ end of the siRNA/shRNA, the 3’ end of the siRNA/shRNA affect the RNAi efficiency. <br><br>
+
 
−
In addition, we tested the attraction effect of sucrose and lemon yellow on P. S. Results show that sucrose plus lemon yellow has the best attraction effect. </p>
+
−
<h1>
+
−
Project-specific biosafety
+
−
</h1>
+
 
<p id="para">
 
<p id="para">
−
In our project, we work with Phyllotreta striolata, which is not on the white list. We submitted check-in form about using Phyllotreta striolata in our project, and got approved by the iGEM Safety and Security Committee. The Phyllotreta striolata we used in our experiment were collected from Shenzhen university’s field station, which is a very well managed and have strict regulations on biosafety and biosecurity.  
+
Short interfering RNAs (siRNAs) and their corresponding shRNAs were designed based on the mRNA sequences of their target genes. siRNAs were synthesized by a bio-company, shRNAs were produced by in vitro transcription. The efficiencies of both siRNAs and shRNAs in mediating RNAi in Phyllotreta striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Results also show that GC percentage of the total siRNA/shRNA, the 5’ end of the siRNA/shRNA, the 3’ end of the siRNA/shRNA affect the RNAi efficiency. <br><br>
−
<br><br>
+
In addition, we tested the attraction effect of sucrose and lemon yellow on P. S. Results show that sucrose plus lemon yellow has the best attraction effect.
−
The product of our project is siRNA/shRNA that are designed to target the genes of Phyllotreta striolata. When we design the siRNAs, we have BLASTed the siRNA sequences against human genome database to avoid the sequences that have high homologous with the sequences of human genes.
+
 
</p>
 
</p>
 +
 +
 +
</body>
 +
</html>
 +
<br><br><br><br><br>

Latest revision as of 23:58, 17 October 2018

Title

Title
Experiments

Experimental SSHS-Shenzhen

Experimental summary

Short interfering RNAs (siRNAs) and their corresponding shRNAs were designed based on the mRNA sequences of their target genes. siRNAs were synthesized by a bio-company, shRNAs were produced by in vitro transcription. The efficiencies of both siRNAs and shRNAs in mediating RNAi in Phyllotreta striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Results also show that GC percentage of the total siRNA/shRNA, the 5’ end of the siRNA/shRNA, the 3’ end of the siRNA/shRNA affect the RNAi efficiency.

In addition, we tested the attraction effect of sucrose and lemon yellow on P. S. Results show that sucrose plus lemon yellow has the best attraction effect.