Difference between revisions of "Team:SSHS-Shenzhen/Experiments"
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{{SSHS-Shenzhen/CSS}} <html lang="en"> <head> | {{SSHS-Shenzhen/CSS}} <html lang="en"> <head> | ||
| − | <meta charset="UTF-8"> <title>Title</title><style> | + | <meta charset="UTF-8"> <title>Title</title> |
| − | + | <style> | |
| − | color: #fff; | + | .header li a:hover,.dropdown:hover.dropbtn { |
| + | color: #fff; | ||
| + | } | ||
| + | .header { | ||
| + | font-size: 30px; | ||
| + | padding: 0px; | ||
| + | margin: 0px; | ||
| + | top: 0; | ||
| + | left: 0; | ||
| + | width: 100%; | ||
| + | position: fixed; | ||
| + | height: 3em; | ||
| + | z-index: 99; | ||
| + | float: left; | ||
| + | background-color: #5d8aa8; | ||
| + | min-width: 1080px; | ||
| + | } | ||
| + | body { | ||
| + | background-color: #fff; | ||
| + | } | ||
| + | .dropdown-content { | ||
| + | display: none; | ||
| + | position: absolute; | ||
| + | background-color: #5d8aa8; | ||
| + | min-width: 250px | ||
| + | } | ||
| + | .dropdown-content a { | ||
| + | color: #f7f7f7; | ||
| + | padding: 12px 16px; | ||
| + | text-decoration: none; | ||
| + | display: block; | ||
| + | font-size: 20px; | ||
| + | } | ||
| + | .header li a,.dropbtn,.header a:link, .header a:visited { | ||
| + | color: #f7f7f7; | ||
| + | } | ||
| + | #para{ | ||
| + | color: #000; | ||
| + | padding:30px 100px 5px; | ||
| + | font-size: 20px!important; | ||
| + | } | ||
| + | #fig{ | ||
| + | color: #000; | ||
| + | padding:30px 100px 5px; | ||
| + | font-size: 20px!important; | ||
| + | text-align: center !important; | ||
| + | } | ||
| + | |||
| + | h1{ | ||
| + | color: #000; | ||
padding:150px 50px 5px!important; font-size: 30px!important; text-align: center; | padding:150px 50px 5px!important; font-size: 30px!important; text-align: center; | ||
} | } | ||
| − | </style> </head> | + | h2{ |
| + | color: #000; | ||
| + | padding:100px 100px 5px; | ||
| + | font-size: 25px!important; | ||
| + | text-align: left; | ||
| + | line-height: 120%; | ||
| + | } | ||
| + | |||
| + | h3{ | ||
| + | color: #000; | ||
| + | padding:100px 100px 5px; | ||
| + | font-size: 25px!important; | ||
| + | text-align: center; | ||
| + | line-height: 120%; | ||
| + | } | ||
| + | |||
| + | .banner2{ | ||
| + | color:#fff; | ||
| + | background-color:#5d8aa8; | ||
| + | font-size:100px!important; | ||
| + | width:100%; height:500px; | ||
| + | text-align:center; | ||
| + | line-height: 500px; | ||
| + | padding:90px 0px 0px; | ||
| + | background-image:url("https://static.igem.org/mediawiki/2018/c/c1/T--SSHS-Shenzhen--test1.jpeg"); | ||
| + | } | ||
| + | </style></head> | ||
<body> | <body> | ||
| − | <div class=" | + | <div class="banner2"> |
Experiments | Experiments | ||
</div> | </div> | ||
| − | < | + | <h1> |
| − | + | Experimental SSHS-Shenzhen | |
| − | < | + | </h1> |
| − | + | ||
| − | < | + | <h1> |
| − | < | + | Experimental summary |
| − | < | + | </h1> |
| − | + | ||
| − | + | <p id="para"> | |
| − | < | + | Short interfering RNAs (siRNAs) and their corresponding shRNAs were designed based on the mRNA sequences of their target genes. siRNAs were synthesized by a bio-company, shRNAs were produced by in vitro transcription. The efficiencies of both siRNAs and shRNAs in mediating RNAi in Phyllotreta striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Results also show that GC percentage of the total siRNA/shRNA, the 5’ end of the siRNA/shRNA, the 3’ end of the siRNA/shRNA affect the RNAi efficiency. <br><br> |
| − | + | In addition, we tested the attraction effect of sucrose and lemon yellow on P. S. Results show that sucrose plus lemon yellow has the best attraction effect. | |
| − | < | + | </p> |
| − | + | ||
</body> | </body> | ||
</html> | </html> | ||
<br><br><br><br><br> | <br><br><br><br><br> | ||
Latest revision as of 23:58, 17 October 2018
Experimental SSHS-Shenzhen
Experimental summary
Short interfering RNAs (siRNAs) and their corresponding shRNAs were designed based on the mRNA sequences of their target genes. siRNAs were synthesized by a bio-company, shRNAs were produced by in vitro transcription. The efficiencies of both siRNAs and shRNAs in mediating RNAi in Phyllotreta striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Results also show that GC percentage of the total siRNA/shRNA, the 5’ end of the siRNA/shRNA, the 3’ end of the siRNA/shRNA affect the RNAi efficiency.
In addition, we tested the attraction effect of sucrose and lemon yellow on P. S. Results show that sucrose plus lemon yellow has the best attraction effect.