Difference between revisions of "Team:Munich/engineering1.html"
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| − | <h4>Transforming E. Coli Rosetta for pRED/ET | + | <h4>Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/04</em> | <em>2018/06/04</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 13: | Line 13: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical | + | <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>Inoculate pRED at 30°C because of temperature sensitive promoter.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>No colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | <h4>Redo: Transforming E. Coli Rosetta for pRED/ET | + | <h4>Redo: Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/08</em> | <em>2018/06/08</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 42: | Line 42: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>No colonies. We decided to redo the experiment by electroporation.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET | + | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/12</em> | <em>2018/06/12</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
| Line 61: | Line 61: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Restriction digest</a> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>No colonies. pRED/ET engineering seems to not work in Rosetta. We postponed this experiment to do it with PD Dr. Lassak at the LMU again.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
| + | <html> | ||
Latest revision as of 00:15, 18 October 2018
Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Chemical transformation |
| Notes: | Inoculate pRED at 30°C because of temperature sensitive promoter. |
| Results: | No colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | Chemical Transformation |
| Notes: | We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN. |
| Results: | No colonies. We decided to redo the experiment by electroporation. |
Redo:Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest |
| Notes: | We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN. |
| Results: | No colonies. pRED/ET engineering seems to not work in Rosetta. We postponed this experiment to do it with PD Dr. Lassak at the LMU again. |