Difference between revisions of "Team:Munich/engineering1.html"
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| − | <h4>Transforming E. Coli Rosetta for pRED/ET | + | <h4>Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/04</em> | <em>2018/06/04</em> | ||
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| − | <h4>Redo: Transforming E. Coli Rosetta for pRED/ET | + | <h4>Redo: Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/08</em> | <em>2018/06/08</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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| − | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET | + | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET Engineering</h4> |
<em>2018/06/12</em> | <em>2018/06/12</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
Latest revision as of 00:15, 18 October 2018
Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Chemical transformation |
| Notes: | Inoculate pRED at 30°C because of temperature sensitive promoter. |
| Results: | No colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | Chemical Transformation |
| Notes: | We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN. |
| Results: | No colonies. We decided to redo the experiment by electroporation. |
Redo:Transforming E. Coli Rosetta for pRED/ET Engineering
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | Restriction digest |
| Notes: | We used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN. |
| Results: | No colonies. pRED/ET engineering seems to not work in Rosetta. We postponed this experiment to do it with PD Dr. Lassak at the LMU again. |