Difference between revisions of "Team:Munich/preparingge.html"
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| − | <h4>Transforming E. Coli DH5a to | + | <h4>Transforming E. Coli DH5a to Amplify Plasmids for pRED/ET Engineering</h4> |
<em>2018/08/28</em> | <em>2018/08/28</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank"> | + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td>Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
<td>~200 colonies</td> | <td>~200 colonies</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
<html> | <html> | ||
Latest revision as of 00:21, 18 October 2018
Transforming E. Coli DH5a to Amplify Plasmids for pRED/ET Engineering
2018/08/28| Participants: | Dominic Schwarz |
| Protocol: | Electrocompetent transformation |
| Notes: | Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid. |
| Results: | ~200 colonies |