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<h4>Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering</h4>
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<h4>Transforming E. Coli DH5a to Amplify Plasmids for pRED/ET Engineering</h4>
 
<em>2018/08/28</em>
 
<em>2018/08/28</em>
 
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       <td>Notes:</td>
 
       <td>Notes:</td>
       <td>inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.</td>
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       <td>Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.</td>
 
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       <td>Results:</td>
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       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
 
       <td>~200 colonies</td>
 
       <td>~200 colonies</td>
 
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Latest revision as of 00:21, 18 October 2018

Transforming E. Coli DH5a to Amplify Plasmids for pRED/ET Engineering

2018/08/28
Participants: Dominic Schwarz
Protocol: Electrocompetent transformation
Notes: Inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.
Results: ~200 colonies