Difference between revisions of "Team:Munich/deletioncasette2.html"
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<div class="event-info"> | <div class="event-info"> | ||
| − | + | <h4>Creating a Selection Cassette from pSB1C3</h4> | |
| − | <h4>Creating a | + | <em>2018/08/28</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, Agarose | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>Results:</td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | <td> | + | <td>2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | |||
| + | <figure class="figure"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/2/2d/T--Munich--Labbook_20180920_prepgel_msbbCAT_recBCDCAT_recBCDCAT%2BFRT.jpeg" class="figure-img img-fluid rounded" alt="something"> | ||
| + | <figcaption class="figure-caption"></figcaption> | ||
| + | </figure> | ||
| + | |||
| + | |||
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</div> | </div> | ||
| + | <html> | ||
Latest revision as of 00:23, 18 October 2018
Creating a Selection Cassette from pSB1C3
2018/08/28| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Knockouts of both msb-B, RecBCD Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb |
| Results: | 2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect. |
