Difference between revisions of "Team:Munich/deletioncasette2.html"

 
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<h4>Creating a selection cassette from pSB1C3</h4>
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<h4>Creating a Selection Cassette from pSB1C3</h4>
 
<em>2018/08/28</em>
 
<em>2018/08/28</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
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       <td>Participants:</td>
 
       <td>Participants:</td>
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       <td>Dominic</td>
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       <td>Dominic Schwarz</td>
 
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     </tr>
 
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       <td>Protocol:</td>
 
       <td>Protocol:</td>
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       <td>PCR, Agarose Gel, Gelextraction</td>
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       <td>
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<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,  
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<a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,  
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<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>
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     </tr>
 
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  <tr>
 
  <tr>
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       <td>Results:</td>
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       <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td>
 
       <td>2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect.</td>
 
       <td>2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect.</td>
 
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Latest revision as of 00:23, 18 October 2018

Creating a Selection Cassette from pSB1C3

2018/08/28
Participants: Dominic Schwarz
Protocol: PCR, Agarose gel, Gel extraction
Notes: Knockouts of both msb-B, RecBCD Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb
Results: 2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect.
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