Difference between revisions of "Team:Munich/GenomicDNA.html"
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| − | + | <h4>Genomic DNA Extraction from E. Coli Rosetta</h4> | |
| − | <h4> | + | <em>2018/05/18</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Enikö Baligács</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>Invitrogen purelink genomic DNA | + | <td>Invitrogen purelink genomic DNA Mini kit</td> |
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</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>PCR Amplification of RecB, C & D from E. Coli Rosetta</h4> | |
| − | <h4>PCR | + | <em>2018/05/24</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Enikö Baligács</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR</td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td>RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) | + | <td>RecC: RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) <br> |
| − | RecB_extract_rv; RecD_extract_fw (AT: 69°C) | + | RecBCD: RecB_extract_rv; RecD_extract_fw (AT: 69°C) |
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</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><a href="https://2018.igem.org/Team:Munich/Results" target="_blank">Results:</a></td> |
| − | + | <td><figure class="figure"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/2/2a/T--munich--diesmalrichtig_RecC-RecBD_extract.jpg " class="figure-img img-fluid rounded" alt=" "> | |
| − | </ | + | <figcaption class="figure-caption">left: RecC, right: recBD |
| − | + | </figcaption> | |
| − | < | + | </figure> |
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</td> | </td> | ||
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</table> | </table> | ||
</div> | </div> | ||
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Latest revision as of 00:28, 18 October 2018
Genomic DNA Extraction from E. Coli Rosetta
2018/05/18| Participants: | Enikö Baligács |
| Protocol: | Invitrogen purelink genomic DNA Mini kit |
PCR Amplification of RecB, C & D from E. Coli Rosetta
2018/05/24| Participants: | Enikö Baligács |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | RecC: RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) RecBCD: RecB_extract_rv; RecD_extract_fw (AT: 69°C) |
| Results: | 
|