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<p>Our project started with the observation that chemical contamination of surface water is an increasingly important problem. We felt that a biobased sensor would offer excellent opportunities for detection of such contaminants, and identified the bacterial chemotaxis pathway as the best point of entry to engineer a flexible and sensitive sensor. Below, we describe the design and implementation of our project.</p> | <p>Our project started with the observation that chemical contamination of surface water is an increasingly important problem. We felt that a biobased sensor would offer excellent opportunities for detection of such contaminants, and identified the bacterial chemotaxis pathway as the best point of entry to engineer a flexible and sensitive sensor. Below, we describe the design and implementation of our project.</p> | ||
<h4>Overview</h4> | <h4>Overview</h4> | ||
− | <p>The goal of our project is to engineer the chemotaxis pathway of the bacterium Escherichia coli to emit a visible light signal that lets us know whether specific contaminants are present in water, and at what levels.</p> | + | <p>The goal of our project is to engineer the chemotaxis pathway of the bacterium <i>Escherichia coli</i> to emit a visible light signal that lets us know whether specific contaminants are present in water, and at what levels.</p> |
</div> | </div> | ||
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<figure class = "zoom" style = "width:40%;float: right;"> | <figure class = "zoom" style = "width:40%;float: right;"> | ||
<img width = 100% src="https://static.igem.org/mediawiki/2018/f/f7/T--Utrecht--2018-Figure1-ProjectDesign.svg" alt="BRET_Assay.png"> | <img width = 100% src="https://static.igem.org/mediawiki/2018/f/f7/T--Utrecht--2018-Figure1-ProjectDesign.svg" alt="BRET_Assay.png"> | ||
− | <figcaption>Figure 1: The Chemotaxis Pathway of <i>E. coli</i>. A) The | + | <figcaption>Figure 1: The Chemotaxis Pathway of <i>E. coli</i>. A) The inactive pathway. B) The active pathway. |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
− | <p>In their natural environment, the chemotaxis system is used by <i>Escherichia coli</i> bacteria to direct them towards the highest concentration of a ligand, or away from a harmful substance. This system uses a receptor that can be modified to detect a wealth of compounds (requirement III), (Shuangyu Bi et al. 2016). Furthermore, the sensitivity of this receptor can be fine tuned through methylation. Customizing the methylation states facilitates the ability to measure a broad concentration range (requirement II). Ultimately, the chemotaxis pathway controls the direction of rotation of whip-like proteins on the bacterial surface called flagella. When these flagella turn counterclockwise, they act in synergy causing the bacteria to swim coherently in one direction. But when they turn clockwise, the bacteria tumbles around, pointing it in a new direction.</p> | + | <p>In their natural environment, the chemotaxis system is used by <i>Escherichia coli</i> bacteria to direct them towards the highest concentration of a ligand, or away from a harmful substance. This system uses a receptor that can be modified to detect a wealth of compounds (requirement III), (Shuangyu Bi et al. 2016). Furthermore, the sensitivity of this receptor can be fine-tuned through methylation. Customizing the methylation states facilitates the ability to measure a broad concentration range (requirement II). Ultimately, the chemotaxis pathway controls the direction of rotation of whip-like proteins on the bacterial surface called flagella. When these flagella turn counterclockwise, they act in synergy causing the bacteria to swim coherently in one direction. But when they turn clockwise, the bacteria tumbles around, pointing it in a new direction.</p> |
<p>When no ligand is bound to the chemotaxis receptor, it continuously activates a signal transduction pathway involving several proteins, including CheA, CheY, and CheZ (figure 1). CheA phosphorylates CheY, which subsequently translocates to the cell membrane where it binds the flagellar motor protein, thereby altering its rotational direction to the “running” state. After CheY is bound to the flagellar motor protein, it is subsequently dephosphorylated by CheZ (otherwise CheY would remain active indefinitely). Upon ligand binding, the receptor is inactivated, leading to an inactivated pathway and a rotational direction of the flagellar motor protein in the “tumbling” state. Since the binding of CheY and CheZ is linked to ligand binding of the receptor, measurement of the binding of these proteins using a Bioluminescence Resonance Energy Transfer (BRET) assay (described below) provides a quick and accurate indication of the concentration of ligand present (requirement I and II).</p> | <p>When no ligand is bound to the chemotaxis receptor, it continuously activates a signal transduction pathway involving several proteins, including CheA, CheY, and CheZ (figure 1). CheA phosphorylates CheY, which subsequently translocates to the cell membrane where it binds the flagellar motor protein, thereby altering its rotational direction to the “running” state. After CheY is bound to the flagellar motor protein, it is subsequently dephosphorylated by CheZ (otherwise CheY would remain active indefinitely). Upon ligand binding, the receptor is inactivated, leading to an inactivated pathway and a rotational direction of the flagellar motor protein in the “tumbling” state. Since the binding of CheY and CheZ is linked to ligand binding of the receptor, measurement of the binding of these proteins using a Bioluminescence Resonance Energy Transfer (BRET) assay (described below) provides a quick and accurate indication of the concentration of ligand present (requirement I and II).</p> | ||
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<li>Measuring pathway activity. The activity of the chemotaxis pathway serves as a proxy for the concentration of a contaminant in water. To measure this activity, we developed a sensor based on the principle of Bioluminescence Resonance Energy Transfer (BRET). Two proteins in the chemotaxis pathway either bind or unbind depending on the presence of ligand, and we measure this binding/unbinding as a change in the color of visible light emitted by the bacteria.</li> | <li>Measuring pathway activity. The activity of the chemotaxis pathway serves as a proxy for the concentration of a contaminant in water. To measure this activity, we developed a sensor based on the principle of Bioluminescence Resonance Energy Transfer (BRET). Two proteins in the chemotaxis pathway either bind or unbind depending on the presence of ligand, and we measure this binding/unbinding as a change in the color of visible light emitted by the bacteria.</li> | ||
− | <li>Detecting diverse ligands. In order to detect a range of ligands, DeTaXion will ultimately make use of ligand binding domains from different surface receptors | + | <li>Detecting diverse ligands. In order to detect a range of ligands, DeTaXion will ultimately make use of ligand binding domains from different surface receptors. Modification of the ligand specificity of these domains are based on prediction using binding-affinity prediction software such as Haddock. Within the scope of this project however, we test the possibility of swapping ligand binding domains between different receptors.</li> |
− | <li>Fine tuning pathway activity. In order to be able to measure a broad range of contaminant concentrations, we make use of an inherent feature of the chemotaxis pathway. Methylation on four different sites of the receptor alters the activity of the pathway at a given ligand concentration. By mimicking these methylations, we can alter the sensitivity of our system to a particular ligand, broadening the dynamic range of detection.</li> | + | <li>Fine-tuning pathway activity. In order to be able to measure a broad range of contaminant concentrations, we make use of an inherent feature of the chemotaxis pathway. Methylation on four different sites of the receptor alters the activity of the pathway at a given ligand concentration. By mimicking these methylations, we can alter the sensitivity of our system to a particular ligand, broadening the dynamic range of detection.</li> |
</ol> | </ol> | ||
− | <p>To create a biosensor meeting all five listed requirements, the <i>E. coli</i> chemotaxis pathway was customized using a three step approach. First, we developed a sensor based on the principle of Bioluminescence Resonance Energy Transfer (BRET). BRET provides a clear and easily measurable detection signal (requirement II and IV). Next, we tested the effectiveness of swapping ligand binding domains with the one of the chemotaxis receptors, the Tar receptor. This enables the possibility to detect diverse ligands (requirement III). The last step of the approach facilitates accurate detection at different concentrations (requirement I). We | + | <p>To create a biosensor meeting all five listed requirements, the <i>E. coli</i> chemotaxis pathway was customized using a three step approach. First, we developed a sensor based on the principle of Bioluminescence Resonance Energy Transfer (BRET). BRET provides a clear and easily measurable detection signal (requirement II and IV). Next, we tested the effectiveness of swapping ligand binding domains with the one of the chemotaxis receptors, the Tar receptor. This enables the possibility to detect diverse ligands (requirement III). The last step of the approach facilitates accurate detection at different concentrations (requirement I). We accomplished this by mimicking receptor methylation, which alters the sensitivity of the chemotaxis pathway.</p> |
<figure class = "zoom" style = "width:40%;float: right;"> | <figure class = "zoom" style = "width:40%;float: right;"> | ||
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− | <p> | + | <p>To create this BRET-pair, two biobricks were created: one biobrick containing fusing the RLuc fused to CheZ (BBa_K2736102<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736102"></a>) and a biobrick containing eYFP fused to CheY (BBa_K2736100<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736100"></a>). The bioluminescence of the BRET-pair can be used as a proxy to demonstrate whether the chemotaxis proteins interact (Figure 3). When no ligand is bound to the Tar receptor, CheA is active and phosphorylates CheY. This enables the phosphorylated eYFP::CheY to interact with CheZ::Rluc, bringing RLuc and eYFP in close proximity of each other. This can be measured as a BRET signal when the RLuc substrate is added. Upon binding of a ligand to the Tar receptor, CheA becomes inactivated, leading to a decrease of the BRET signal. Importantly, the signal can be readily measured by using an affordable bioluminescence assay.</p> |
− | <h4> Detection of a range of ligands </h4> | + | <h4 id = "Receptor"> Detection of a range of ligands </h4> |
− | <p>DeTaXion will ultimately make use of ligand binding domains from different surface receptors, and further modify the ligand specificity of these domains based on prediction using binding-affinity prediction software such as Haddock. Within the scope of this project however, we test the possibility of swapping ligand binding domains to change the type of ligand detected. We fused the Tar ligand binding domain (LBD) to the two-component copper sensitive (CusS) receptor. This new hybrid receptor protein detects aspartate instead of copper, and serves as a proof of concept for the ability to create hybrid receptors by exchange of LBDs from two different receptors. The functionality of the fusion protein was verified by using the downstream | + | <p>DeTaXion will ultimately make use of ligand binding domains from different surface receptors, and further modify the ligand specificity of these domains based on prediction using binding-affinity prediction software such as Haddock. Within the scope of this project however, we test the possibility of swapping ligand binding domains to change the type of ligand detected. We fused the Tar ligand binding domain (LBD) to the two-component copper sensitive (CusS) receptor (BBa_K2736109<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736109"></a>). This new hybrid receptor protein detects aspartate instead of copper, and serves as a proof of concept for the ability to create hybrid receptors by exchange of LBDs from two different receptors. The functionality of the fusion protein was verified by using the downstream CusS pathway. </p> |
<p>When a ligand specific for the LBD is bound to the receptor, a signal is transduced to the response regulator protein (CusR) which binds to and activates a copper sensitive promoter. Normally, the CusR promoter activates genes involved in copper resistance protecting the bacterium against this potentially harmful transition metal. </p> | <p>When a ligand specific for the LBD is bound to the receptor, a signal is transduced to the response regulator protein (CusR) which binds to and activates a copper sensitive promoter. Normally, the CusR promoter activates genes involved in copper resistance protecting the bacterium against this potentially harmful transition metal. </p> | ||
− | <p>In this experiment, the promoter region was coupled to a red fluorescent protein (RFP) sequence. Thus, when aspartate is bound to the receptor, CusR is activated | + | <p>In this experiment, the promoter region was coupled to a red fluorescent protein (RFP) sequence. Thus, when aspartate is bound to the receptor, CusR is activated, and the downstream promoter and RFP are transcribed. This way, the expression of RFP serves as an indicator for a functional Tar-CusS receptor. RFP was used as indicator as it is easily measured using fluorescent light microscopy. The custom receptor was ordered at Integrated DNA Technologies (IDT)(BBa_K2736109<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736109"></a>) while the CusR promotor (BBa_K608003<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736106"></a>) and RFP (BBa_K516032<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K516032"></a>) were transformed from the iGEM biobrick kit.</p> |
− | <h4>Fine tuning of sensor sensitivity </h4> | + | <h4 id = "Methylation">Fine tuning of sensor sensitivity </h4> |
− | <p><a class = "zoom" href = "https://2018.igem.org/Team:Utrecht/Human_Practices">Jos van der Vosse (TNO)</a> suggested quantifiable measurements. We therefore decided to implement an additional feature into our biosensor, facilitating the measurement of multiple ranges of concentrations. This method is based on the methylation state of the Tar receptor, which influences its sensitivity to ligands.</p> | + | <p><a class = "zoom" href = "https://2018.igem.org/Team:Utrecht/Human_Practices#TNO">Jos van der Vosse (TNO)</a> suggested quantifiable measurements. We therefore decided to implement an additional feature into our biosensor, facilitating the measurement of multiple ranges of concentrations. This method is based on the methylation state of the Tar receptor, which influences its sensitivity to ligands.</p> |
<p>In the unmethylated state, the receptor transduces the ligand binding signal to an inactivated pathway (Figure 4A and 4B). Upon receptor methylation, the conformational change required to transduce the inactivation signal, is energetically less favorable, resulting in lower occurrence. Therefore, when the same amount of ligand is present, the level of pathway inactivation is lower than in the unmethylated receptor state. Since the Tar receptor has four acidic residues that can be methylated (Q295, E302, Q309, E491), there are in total sixteen different combinations of methylation states, each one resulting in a slightly different sensitivity (Figure 4C).</p> | <p>In the unmethylated state, the receptor transduces the ligand binding signal to an inactivated pathway (Figure 4A and 4B). Upon receptor methylation, the conformational change required to transduce the inactivation signal, is energetically less favorable, resulting in lower occurrence. Therefore, when the same amount of ligand is present, the level of pathway inactivation is lower than in the unmethylated receptor state. Since the Tar receptor has four acidic residues that can be methylated (Q295, E302, Q309, E491), there are in total sixteen different combinations of methylation states, each one resulting in a slightly different sensitivity (Figure 4C).</p> | ||
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<ol> | <ol> | ||
<li>The wild type state, the unmethylated Tar receptor (QEQE, most sensitive);</li> | <li>The wild type state, the unmethylated Tar receptor (QEQE, most sensitive);</li> | ||
− | <li>A Tar receptor with one mimicked methylation site (QEAE, average sensitivity);</li> | + | <li>A Tar receptor with one mimicked methylation site (QEAE, average sensitivity, BBa_K2736108<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736108"></a>);</li> |
− | <li> | + | <li>Another Tar receptor with one mimicked methylation site(QEQA, least sensitive, BBa_K2736106<a class = "url_icon" href = "http://parts.igem.org/Part:BBa_K2736106"></a>).</li> |
</ol> | </ol> | ||
− | <p>The Tar receptor that we received from a collaboration with <a href = "https://2018.igem.org/Team:Utrecht/Collaborations">iGEM team Groningen</a> was mutated using site-directed mutagenesis and expressed in <i>E. coli </i> strains UU1250 and VS181, which do not express any chemotaxis receptors. In addition, VS181 does not express the methyltransferases CheW and CheB, resulting in a fixed methylation state. The <i>E. coli</i> strains were kindly provided by Shuangyu Bi from the lab of V. Sourjik at the Max Planck Institute for Terrestrial Microbiology in Marburg.</p> | + | <p>The Tar receptor that we received from a collaboration with <a href = "https://2018.igem.org/Team:Utrecht/Collaborations#Tar">iGEM team Groningen</a> was mutated using site-directed mutagenesis and expressed in <i>E. coli </i> strains UU1250 and VS181, which do not express any chemotaxis receptors. In addition, VS181 does not express the methyltransferases CheW and CheB, resulting in a fixed methylation state. The <i>E. coli</i> strains were kindly provided by Shuangyu Bi from the lab of V. Sourjik at the Max Planck Institute for Terrestrial Microbiology in Marburg.</p> |
− | <p>Next, the sensitivity of <i>E. coli</i> for aspartate | + | <p>Next, the sensitivity of <i>E. coli</i> for aspartate can be measured. This can be achieved by addition of a range of aspartate concentrations and subsequent luminescence measurements by using the self-designed BRET-pair. <a href = "https://2018.igem.org/Team:Utrecht/Model">Our model</a> can be verified and corrected based on these measurements. BRET measurements can be validated using a FRET-pair received from Marburg.</p> |
</div> | </div> | ||
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<div class = "customelementM5B"> | <div class = "customelementM5B"> | ||
<div class = "references"> | <div class = "references"> | ||
+ | <h4> References </h4> | ||
Cui B, Wang Y, Song Y, et al. (2014) Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria. mBio. 5(3):e01050-14. DOI:10.1128/mBio.01050-14.</br> | Cui B, Wang Y, Song Y, et al. (2014) Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria. mBio. 5(3):e01050-14. DOI:10.1128/mBio.01050-14.</br> | ||
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<div class = "navigateArrows"> | <div class = "navigateArrows"> | ||
<div class = "arrowLeft"> <a href="https://2018.igem.org/Team:Utrecht/Applied_Design"><b class = "fa fa-angle-left"></b> Applied Design</a></div> | <div class = "arrowLeft"> <a href="https://2018.igem.org/Team:Utrecht/Applied_Design"><b class = "fa fa-angle-left"></b> Applied Design</a></div> | ||
− | <div class = "arrowLeft"> <a href="https://2018.igem.org/Team:Utrecht/ | + | <div class = "arrowLeft"> <a href="https://2018.igem.org/Team:Utrecht/Description"><b class = "fa fa-angle-double-left"></b> Project Description</a></div> |
− | <div class = "arrowRight"> <a href="https://2018.igem.org/Team:Utrecht/ | + | <div class = "arrowRight"> <a href="https://2018.igem.org/Team:Utrecht/Results">Results <b class = "fa fa-angle-double-right"></b></a></div> |
− | <div class = "arrowRight"> <a href="https://2018.igem.org/Team:Utrecht/ | + | <div class = "arrowRight"> <a href="https://2018.igem.org/Team:Utrecht/Parts">Biobricks <b class = "fa fa-angle-right"></b></a></div> |
</div> | </div> | ||
Latest revision as of 01:09, 18 October 2018