Difference between revisions of "Team:Edinburgh UG/Improve"

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{{Edinburgh_UG}}
 
{{Edinburgh_UG}}
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<div class="column full_size judges-will-not-evaluate">
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  <head>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="clear"></div>
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    <title>Edinburgh iGEM 2018</title>
  
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    <!-- Bootstrap core CSS -->
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    <link href="https://2018.igem.org/Team:Edinburgh_UG/bootstrapmincss?action=raw&ctype=text/css" rel="stylesheet">
  
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<div class="column full_size">
 
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<h1>Improve</h1>
 
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
 
  
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<h3>Gold Medal Criterion #2</h3>
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    <!-- Custom fonts for this template -->
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
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    <link href="https://2018.igem.org/Team:Edinburgh_UG/fontawesome?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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    <!-- Custom styles for this template -->
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    <link href="https://2018.igem.org/Team:Edinburgh_UG/home_css?action=raw&c" rel="stylesheet">
  
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<br><br>
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  </head>
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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  <body id="page-top">
  
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</div>
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    <!-- Menu -->
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    <nav class="navbar navbar-expand-lg navbar-dark bg-dark">
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                  <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Description">Description</a>
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            </li>
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      </div>
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    </nav>
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    <!-- Intro Header -->
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    <header class="masthead">
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      <div class="intro-body">
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        <div class="container">
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          <div class="row">
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            <div class="col-lg-8 mx-auto">
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              <h1 class="brand-heading" align="center">Improve</h1>
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              <p class="intro-text"></p>
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            </div>
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          </div>
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        </div>
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    </header>
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    <section id="about" class="content-section text-center">
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      <div class="container">
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        <div class="row">
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          <div class="col-lg-8 mx-auto">
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            <h2 style="text-align:left">BBa_K2725001</h2>
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            <p style="text-align:left">We improved the characterisation of a previous part: <a href="http://parts.igem.org/Part:BBa_K771303">BBa_K771303</a>, which contains the
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coding sequence of E. coli FabI. FabI is involved in type 2 fatty acid synthesis and has
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previously been characterised as a biosensor for triclosan, a common biocide.
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Overexpression of FabI has been shown to give resistance to triclosan, so we characterised
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the use of FabI as an alternative selection gene. Usage of triclosan instead of antibiotics is
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considerably cheaper, and safer for the environment.</p>
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          </div>
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        </div>
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      </div>
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    </section>
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    <section id="about" class="content-section text-center">
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      <div class="container">
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        <div class="row">
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          <div class="col-lg-8 mx-auto">
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            <h2 style="text-align:left">BBa_K914009 & BBa_K914018</h2>
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            <p style="text-align:left">We improved the characterisation of the previous parts: <a href="http://parts.igem.org/Part:BBa_K914009">BBa_K914009</a> &amp; <a href="http://parts.igem.org/Part:BBa_K914018">BBa_K914018</a>,
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which contain a kanamycin resistance gene with one and two serine -&gt; amber codon
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mutations, respectively. Previously, their kanamycin resistance with and without a
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suppressor tRNA were characterised in E. coli MG1655. We further characterised their
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ability to confer resistance in E. coli TOP10 and made further parts with a larger number of
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amber mutations.</p>
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          </div>
 +
        </div>
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      </div>
 +
    </section>
 +
 +
    <section id="about" class="content-section text-center">
 +
      <div class="container">
 +
        <div class="row">
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          <div class="col-lg-8 mx-auto">
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            <h2 style="text-align:left">BBa_K2725012 & BBa_K2725013</h2>
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            <p style="text-align:left">We improved the previous parts: <a href="http://parts.igem.org/Part:BBa_K914009">BBa_K914009</a> &amp; <a href="http://parts.igem.org/Part:BBa_K914018">BBa_K914018</a>, which contain a kanamycin
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resistance gene with one and two serine -&gt; amber codon mutations, respectively. By
 +
increasing the number of serine -&gt; amber codon mutations to 5 in <a href="http://parts.igem.org/Part:BBa_K2725012">BBa_K2725012</a> and to 10
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in <a href="http://parts.igem.org/Part:BBa_K2725013">BBa_K2725013</a>, they confer a decreased kanamycin resistance in the absence of a
 +
suppressor tRNA, reducing the leakiness of the gene and thereby increasing its safety.</p>
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          </div>
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        </div>
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      </div>
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    </section>
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    <!-- Contact Section -->
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            <h2>Contact EdiGEM18</h2>
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Latest revision as of 01:30, 18 October 2018

Edinburgh iGEM 2018

Improve

BBa_K2725001

We improved the characterisation of a previous part: BBa_K771303, which contains the coding sequence of E. coli FabI. FabI is involved in type 2 fatty acid synthesis and has previously been characterised as a biosensor for triclosan, a common biocide. Overexpression of FabI has been shown to give resistance to triclosan, so we characterised the use of FabI as an alternative selection gene. Usage of triclosan instead of antibiotics is considerably cheaper, and safer for the environment.

BBa_K914009 & BBa_K914018

We improved the characterisation of the previous parts: BBa_K914009 & BBa_K914018, which contain a kanamycin resistance gene with one and two serine -> amber codon mutations, respectively. Previously, their kanamycin resistance with and without a suppressor tRNA were characterised in E. coli MG1655. We further characterised their ability to confer resistance in E. coli TOP10 and made further parts with a larger number of amber mutations.

BBa_K2725012 & BBa_K2725013

We improved the previous parts: BBa_K914009 & BBa_K914018, which contain a kanamycin resistance gene with one and two serine -> amber codon mutations, respectively. By increasing the number of serine -> amber codon mutations to 5 in BBa_K2725012 and to 10 in BBa_K2725013, they confer a decreased kanamycin resistance in the absence of a suppressor tRNA, reducing the leakiness of the gene and thereby increasing its safety.

Contact EdiGEM18

Feel free to leave us a comment on social media!