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<div class="dropdown-menu" aria-labelledby="navbarDropdownMenuLink"> | <div class="dropdown-menu" aria-labelledby="navbarDropdownMenuLink"> | ||
<a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Parts">Parts Overview</a> | <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Parts">Parts Overview</a> | ||
− | + | ||
− | + | <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Basic_Part">Basic Parts</a> | |
− | + | <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Composite_Part">Composite Parts</a> | |
+ | <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Part_Collection">Part Collection</a> | ||
+ | |||
</div> | </div> | ||
</li> | </li> | ||
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<a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Safety">Safety <span class="sr-only">(current)</span></a> | <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Safety">Safety <span class="sr-only">(current)</span></a> | ||
</li> | </li> | ||
− | <li class="nav-item | + | <li class="nav-item active"> |
− | <a class="nav-link | + | <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Human_Practices">Human Practices <span class="sr-only">(current)</span></a> |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </li> | + | |
<li class="nav-item dropdown"> | <li class="nav-item dropdown"> | ||
<a class="nav-link dropdown-toggle" href="#" id="navbarDropdownMenuLink" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> | <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownMenuLink" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> | ||
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<a class="nav-link" href="https://igem.org/2018_Judging_Form?team=Edinburgh_UG">Judging Form <span class="sr-only">(current)</span></a> | <a class="nav-link" href="https://igem.org/2018_Judging_Form?team=Edinburgh_UG">Judging Form <span class="sr-only">(current)</span></a> | ||
</li> | </li> | ||
+ | |||
+ | <li class="nav-item active"> | ||
+ | <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Medal_Criteria">Medal Criteria<span class="sr-only">(current)</span></a> | ||
+ | </li> | ||
+ | |||
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-lg-8 mx-auto"> | <div class="col-lg-8 mx-auto"> | ||
− | <h1 class="brand-heading">Improve</h1> | + | <h1 class="brand-heading" align="center">Improve</h1> |
<p class="intro-text"></p> | <p class="intro-text"></p> | ||
</div> | </div> | ||
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</div> | </div> | ||
</header> | </header> | ||
+ | |||
+ | <section id="about" class="content-section text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-lg-8 mx-auto"> | ||
+ | <h2 style="text-align:left">BBa_K2725001</h2> | ||
+ | <p style="text-align:left">We improved the characterisation of a previous part: <a href="http://parts.igem.org/Part:BBa_K771303">BBa_K771303</a>, which contains the | ||
+ | coding sequence of E. coli FabI. FabI is involved in type 2 fatty acid synthesis and has | ||
+ | previously been characterised as a biosensor for triclosan, a common biocide. | ||
+ | Overexpression of FabI has been shown to give resistance to triclosan, so we characterised | ||
+ | the use of FabI as an alternative selection gene. Usage of triclosan instead of antibiotics is | ||
+ | considerably cheaper, and safer for the environment.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | <section id="about" class="content-section text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-lg-8 mx-auto"> | ||
+ | <h2 style="text-align:left">BBa_K914009 & BBa_K914018</h2> | ||
+ | <p style="text-align:left">We improved the characterisation of the previous parts: <a href="http://parts.igem.org/Part:BBa_K914009">BBa_K914009</a> & <a href="http://parts.igem.org/Part:BBa_K914018">BBa_K914018</a>, | ||
+ | which contain a kanamycin resistance gene with one and two serine -> amber codon | ||
+ | mutations, respectively. Previously, their kanamycin resistance with and without a | ||
+ | suppressor tRNA were characterised in E. coli MG1655. We further characterised their | ||
+ | ability to confer resistance in E. coli TOP10 and made further parts with a larger number of | ||
+ | amber mutations.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | |||
+ | <section id="about" class="content-section text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-lg-8 mx-auto"> | ||
+ | <h2 style="text-align:left">BBa_K2725012 & BBa_K2725013</h2> | ||
+ | <p style="text-align:left">We improved the previous parts: <a href="http://parts.igem.org/Part:BBa_K914009">BBa_K914009</a> & <a href="http://parts.igem.org/Part:BBa_K914018">BBa_K914018</a>, which contain a kanamycin | ||
+ | resistance gene with one and two serine -> amber codon mutations, respectively. By | ||
+ | increasing the number of serine -> amber codon mutations to 5 in <a href="http://parts.igem.org/Part:BBa_K2725012">BBa_K2725012</a> and to 10 | ||
+ | in <a href="http://parts.igem.org/Part:BBa_K2725013">BBa_K2725013</a>, they confer a decreased kanamycin resistance in the absence of a | ||
+ | suppressor tRNA, reducing the leakiness of the gene and thereby increasing its safety.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
<!-- Contact Section --> | <!-- Contact Section --> | ||
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<i class="fa fa-twitter fa-fw"></i> | <i class="fa fa-twitter fa-fw"></i> | ||
<span class="network-name">Twitter</span> | <span class="network-name">Twitter</span> | ||
+ | </a> | ||
+ | </li> | ||
+ | <li class="list-inline-item"> | ||
+ | <a href="https://www.instagram.com/igemedinburgh/" class="btn btn-default btn-lg"> | ||
+ | <i class="fa fa-instagram fa-fw"></i> | ||
+ | <span class="network-name">Instagram</span> | ||
</a> | </a> | ||
</li> | </li> |
Latest revision as of 01:30, 18 October 2018
Improve
BBa_K2725001
We improved the characterisation of a previous part: BBa_K771303, which contains the coding sequence of E. coli FabI. FabI is involved in type 2 fatty acid synthesis and has previously been characterised as a biosensor for triclosan, a common biocide. Overexpression of FabI has been shown to give resistance to triclosan, so we characterised the use of FabI as an alternative selection gene. Usage of triclosan instead of antibiotics is considerably cheaper, and safer for the environment.
BBa_K914009 & BBa_K914018
We improved the characterisation of the previous parts: BBa_K914009 & BBa_K914018, which contain a kanamycin resistance gene with one and two serine -> amber codon mutations, respectively. Previously, their kanamycin resistance with and without a suppressor tRNA were characterised in E. coli MG1655. We further characterised their ability to confer resistance in E. coli TOP10 and made further parts with a larger number of amber mutations.
BBa_K2725012 & BBa_K2725013
We improved the previous parts: BBa_K914009 & BBa_K914018, which contain a kanamycin resistance gene with one and two serine -> amber codon mutations, respectively. By increasing the number of serine -> amber codon mutations to 5 in BBa_K2725012 and to 10 in BBa_K2725013, they confer a decreased kanamycin resistance in the absence of a suppressor tRNA, reducing the leakiness of the gene and thereby increasing its safety.
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