Difference between revisions of "Team:Duke/Results"
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To test the expression levels of our proteins, we used the shake flask protein expression technique. The specific details of the protocol can be found in the protocol section of the website. | To test the expression levels of our proteins, we used the shake flask protein expression technique. The specific details of the protocol can be found in the protocol section of the website. | ||
Prior to performing the SDS-page, we made sure that all the OD600 for our cells (BAPT, DBAT, badA, tax10, tycA) are around 10.0 for consistency of protein amount in SDS-page. | Prior to performing the SDS-page, we made sure that all the OD600 for our cells (BAPT, DBAT, badA, tax10, tycA) are around 10.0 for consistency of protein amount in SDS-page. | ||
| − | After the SDS-page has finished running, the gel is stained and destained and a greyscale image of the gel | + | After the SDS-page has finished running, the gel is stained and destained and a greyscale image of the gel look like the follows: |
<object data="https://static.igem.org/mediawiki/2018/7/78/T--Duke--proteinexpressiongel.pdf" width="100%" height="700"> </object> | <object data="https://static.igem.org/mediawiki/2018/7/78/T--Duke--proteinexpressiongel.pdf" width="100%" height="700"> </object> | ||
| − | As seen from the graph, the | + | As seen from the graph, from the right to the left, the lanes are the ladder (Mark12TM Unstained Standard), BAPT, DBAT, badA, tax10, and tycA). Although the gel was broken during the destaining process, we were able to put the gel back together. To demonstrate the most accurate level of protein expression, we changed the contrast of the image so that only dark bands on the gel can be seen when using the imaging software, the contrasted image is shown below: |
| + | |||
| + | Using imageJ, we highlighted each | ||
</p> | </p> | ||
Revision as of 01:32, 18 October 2018
Keng Decribe Protein Expression Gels
Joe Decribe Enzyme Assays
Results
Here you can describe the results of your project and your future plans.
Protein Expression Results
To test the expression levels of our proteins, we used the shake flask protein expression technique. The specific details of the protocol can be found in the protocol section of the website. Prior to performing the SDS-page, we made sure that all the OD600 for our cells (BAPT, DBAT, badA, tax10, tycA) are around 10.0 for consistency of protein amount in SDS-page. After the SDS-page has finished running, the gel is stained and destained and a greyscale image of the gel look like the follows: As seen from the graph, from the right to the left, the lanes are the ladder (Mark12TM Unstained Standard), BAPT, DBAT, badA, tax10, and tycA). Although the gel was broken during the destaining process, we were able to put the gel back together. To demonstrate the most accurate level of protein expression, we changed the contrast of the image so that only dark bands on the gel can be seen when using the imaging software, the contrasted image is shown below: Using imageJ, we highlighted each
What should this page contain?
- Clearly and objectively describe the results of your work.
- Future plans for the project.
- Considerations for replicating the experiments.
Describe what your results mean
- Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
- Show data, but remember all measurement and characterization data must be on part pages in the Registry.
- Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.