Difference between revisions of "Team:Edinburgh UG/Part Collection"

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{{Edinburgh_UG}}
 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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    <title>Edinburgh iGEM 2018</title>
  
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<h1> Part Collection </h1>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.  
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<h3>Note</h3>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<h3>Best Part Collection Special Prize</h3>
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<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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              <h1 class="brand-heading" align="center">Part Collection</h1>
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              <p class="intro-text"></p>
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              <h3 class="brand-heading">Semantic Containment System</h3>
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              <p class="intro-text"> These parts collectively provide proof of concept for a semantic containment system that can prevent a horizontally transferred gene being read and expressed by any environmental cell that has picked it up. Our <a href="https://2018.igem.org/Team:Edinburgh_UG/Semantic_Containment_Modelling"> probability modelling </a> predicts that a gene that has 5 and 10 amber STOP codons substituted into the coding sequence will only be expressed 1 in every 10<sup>10</sup> and 1 in every 10<sup>18</sup> translation attempts. This is confirmed by growth curves for Top10 E. coli transformed with  <a href="http://parts.igem.org/Part:BBa_K2725012"> P1003 5* </a> and  <a href="http://parts.igem.org/Part:BBa_K2725013"> P1003 10*.</a> No kanamycin resistance is observed from these strains. When P1003 5* is expressed alongside <a href="http://parts.igem.org/Part:BBa_K2725016"> J23108-supD </a>, high level expression of the P1003 gene is achieved and confers kanamycin resistance in the strain. These parts together model a functional semantic containment system. </p>
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<div class="container">         
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    <thead>
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      <tr>
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        <th>Part number</th>
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        <th>Type</th>
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        <th>Description</th>
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        <th>Supporting information</th>
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        <th>Part length (bp)</th>
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        <td> <a href="http://parts.igem.org/Part:BBa_K2725012"> BBa_K2725012</a></td>
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        <td>Basic</td>
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        <td>KanR 5*</td>
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        <td>P1003 kanamycin resistance with 5 amber stop codons</td>
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        <td>967</td>
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      </tr>
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        <td> <a href="http://parts.igem.org/Part:BBa_K2725013"> BBa_K2725013</a></td>
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        <td>Basic</td>
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        <td>KanR 10*</td>
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        <td>P1003 kanamycin resistance with 10 amber stop codons</td>
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        <td>967</td>
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      </tr>
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        <td> <a href="http://parts.igem.org/Part:BBa_K2725016"> BBa_K2725016</a></td>
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        <td>Basic</td>
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        <td>J23108 - SupD</td>
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        <td>Serine amber suppressor tRNA gene under J23108 Anderson promoter</td>
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        <td>224</td>
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Latest revision as of 01:36, 18 October 2018

Edinburgh iGEM 2018

Part Collection

Semantic Containment System

These parts collectively provide proof of concept for a semantic containment system that can prevent a horizontally transferred gene being read and expressed by any environmental cell that has picked it up. Our probability modelling predicts that a gene that has 5 and 10 amber STOP codons substituted into the coding sequence will only be expressed 1 in every 1010 and 1 in every 1018 translation attempts. This is confirmed by growth curves for Top10 E. coli transformed with P1003 5* and P1003 10*. No kanamycin resistance is observed from these strains. When P1003 5* is expressed alongside J23108-supD , high level expression of the P1003 gene is achieved and confers kanamycin resistance in the strain. These parts together model a functional semantic containment system.

Part number Type Description Supporting information Part length (bp)
BBa_K2725012 Basic KanR 5* P1003 kanamycin resistance with 5 amber stop codons 967
BBa_K2725013 Basic KanR 10* P1003 kanamycin resistance with 10 amber stop codons 967
BBa_K2725016 Basic J23108 - SupD Serine amber suppressor tRNA gene under J23108 Anderson promoter 224

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