Difference between revisions of "Team:Edinburgh UG/Part Collection"
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| − | <h1 class="brand-heading">Part Collection</h1> | + | <h1 class="brand-heading" align="center">Part Collection</h1> |
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| − | <h3 class="brand-heading"> | + | <h3 class="brand-heading">Semantic Containment System</h3> |
| − | <p class="intro-text"> | + | <p class="intro-text"> These parts collectively provide proof of concept for a semantic containment system that can prevent a horizontally transferred gene being read and expressed by any environmental cell that has picked it up. Our <a href="https://2018.igem.org/Team:Edinburgh_UG/Semantic_Containment_Modelling"> probability modelling </a> predicts that a gene that has 5 and 10 amber STOP codons substituted into the coding sequence will only be expressed 1 in every 10<sup>10</sup> and 1 in every 10<sup>18</sup> translation attempts. This is confirmed by growth curves for Top10 E. coli transformed with <a href="http://parts.igem.org/Part:BBa_K2725012"> P1003 5* </a> and <a href="http://parts.igem.org/Part:BBa_K2725013"> P1003 10*.</a> No kanamycin resistance is observed from these strains. When P1003 5* is expressed alongside <a href="http://parts.igem.org/Part:BBa_K2725016"> J23108-supD </a>, high level expression of the P1003 gene is achieved and confers kanamycin resistance in the strain. These parts together model a functional semantic containment system. </p> |
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Latest revision as of 01:36, 18 October 2018
Part Collection
Semantic Containment System
These parts collectively provide proof of concept for a semantic containment system that can prevent a horizontally transferred gene being read and expressed by any environmental cell that has picked it up. Our probability modelling predicts that a gene that has 5 and 10 amber STOP codons substituted into the coding sequence will only be expressed 1 in every 1010 and 1 in every 1018 translation attempts. This is confirmed by growth curves for Top10 E. coli transformed with P1003 5* and P1003 10*. No kanamycin resistance is observed from these strains. When P1003 5* is expressed alongside J23108-supD , high level expression of the P1003 gene is achieved and confers kanamycin resistance in the strain. These parts together model a functional semantic containment system.
| Part number | Type | Description | Supporting information | Part length (bp) |
|---|---|---|---|---|
| BBa_K2725012 | Basic | KanR 5* | P1003 kanamycin resistance with 5 amber stop codons | 967 |
| BBa_K2725013 | Basic | KanR 10* | P1003 kanamycin resistance with 10 amber stop codons | 967 |
| BBa_K2725016 | Basic | J23108 - SupD | Serine amber suppressor tRNA gene under J23108 Anderson promoter | 224 |
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