Difference between revisions of "Team:Edinburgh UG/Part Collection"

 
Line 75: Line 75:
 
                 <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Safety">Safety <span class="sr-only">(current)</span></a>
 
                 <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Safety">Safety <span class="sr-only">(current)</span></a>
 
             </li>
 
             </li>
             <li class="nav-item dropdown">
+
             <li class="nav-item active">
                 <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownMenuLink" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
+
                 <a class="nav-link" href="https://2018.igem.org/Team:Edinburgh_UG/Human_Practices">Human Practices <span class="sr-only">(current)</span></a>
          Human Practices
+
             </li>          
                </a>
+
                <div class="dropdown-menu" aria-labelledby="navbarDropdownMenuLink">
+
                  <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Human_Practices">Human Practices</a>
+
                  <a class="dropdown-item" href="https://2018.igem.org/Team:Edinburgh_UG/Public_Engagement">Education and Engagement</a>
+
                </div>
+
             </li>
+
 
             <li class="nav-item dropdown">
 
             <li class="nav-item dropdown">
 
                 <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownMenuLink" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
 
                 <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownMenuLink" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
Line 110: Line 104:
 
           <div class="row">
 
           <div class="row">
 
             <div class="col-lg-8 mx-auto">
 
             <div class="col-lg-8 mx-auto">
               <h1 class="brand-heading">Part Collection</h1>
+
               <h1 class="brand-heading" align="center">Part Collection</h1>
 
               <p class="intro-text"></p>
 
               <p class="intro-text"></p>
 
             </div>
 
             </div>

Latest revision as of 01:36, 18 October 2018

Edinburgh iGEM 2018

Part Collection

Semantic Containment System

These parts collectively provide proof of concept for a semantic containment system that can prevent a horizontally transferred gene being read and expressed by any environmental cell that has picked it up. Our probability modelling predicts that a gene that has 5 and 10 amber STOP codons substituted into the coding sequence will only be expressed 1 in every 1010 and 1 in every 1018 translation attempts. This is confirmed by growth curves for Top10 E. coli transformed with P1003 5* and P1003 10*. No kanamycin resistance is observed from these strains. When P1003 5* is expressed alongside J23108-supD , high level expression of the P1003 gene is achieved and confers kanamycin resistance in the strain. These parts together model a functional semantic containment system.

Part number Type Description Supporting information Part length (bp)
BBa_K2725012 Basic KanR 5* P1003 kanamycin resistance with 5 amber stop codons 967
BBa_K2725013 Basic KanR 10* P1003 kanamycin resistance with 10 amber stop codons 967
BBa_K2725016 Basic J23108 - SupD Serine amber suppressor tRNA gene under J23108 Anderson promoter 224

Contact EdiGEM18

Feel free to leave us a comment on social media!