Difference between revisions of "Team:Edinburgh UG/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <title>Edinburgh iGEM 2018</title>
  
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve their gold medals!
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Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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              <h1 class="brand-heading" align="center">Demonstrate</h1>
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              <p class="intro-text">All aspects of our project combine to provide a novel chassis for Synthetic Biology: Maxicells. These maxicells are easy to use and can be safely deployed into the environment through the use of our Triple Lock System.</p>
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              <h2 style="text-align:left">Maxicell Characterisation</h2>
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              <ul><li style="text-align:left">We have assessed various methods of maxicell production and have concluded that they are easy to make through UV exposure and homing endonuclease action</li>
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                  <li style="text-align:left">Our knockdown construct <a href="http://parts.igem.org/Part:BBa_K2725008">BBa_K2725008</a> allows maxicell production by theoretically any strain of <i>E. coli</i></li>
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                  <li style="text-align:left">We have shown that maxicells can function as a biosensor</li>
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                  <li style="text-align:left">The active lifespan of maxicells was found to be enough for real-world applications: over 18 hours</li>
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              </ul>
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              <h2 style="text-align:left">The Triple Lock System</h2>
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              <ul><li style="text-align:left">We have developed a model that allows fine-tuning of the maxicell lifespan through the use of a Colicin E2 kill switch</li>
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                  <li style="text-align:left">Semantic containment of our plasmid DNA was demonstrated as a feasible method of preventing expression of our genes by foreign organisms, whilst allowing expression in our chassis. </li>
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                  <li style="text-align:left">Triclosan was successfully used as a selection marker as an alternative to antibiotic resistance and a new, <a href="http://parts.igem.org/Part:BBa_K2725005">triclosan resistant plasmid backbone</a> was produced</li>
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              </ul>
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            <p style="text-align:left"></p>
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            <p style="text-align:left">When put together, our final maxicell product would therefore have virtually no risk to the biosphere upon environmental release.</p>
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            <h2>Contact EdiGEM18</h2>
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            <p>Feel free to leave us a comment on social media!</p>
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Latest revision as of 01:38, 18 October 2018

Edinburgh iGEM 2018

Demonstrate

All aspects of our project combine to provide a novel chassis for Synthetic Biology: Maxicells. These maxicells are easy to use and can be safely deployed into the environment through the use of our Triple Lock System.

Maxicell Characterisation

  • We have assessed various methods of maxicell production and have concluded that they are easy to make through UV exposure and homing endonuclease action
  • Our knockdown construct BBa_K2725008 allows maxicell production by theoretically any strain of E. coli
  • We have shown that maxicells can function as a biosensor
  • The active lifespan of maxicells was found to be enough for real-world applications: over 18 hours

The Triple Lock System

  • We have developed a model that allows fine-tuning of the maxicell lifespan through the use of a Colicin E2 kill switch
  • Semantic containment of our plasmid DNA was demonstrated as a feasible method of preventing expression of our genes by foreign organisms, whilst allowing expression in our chassis.
  • Triclosan was successfully used as a selection marker as an alternative to antibiotic resistance and a new, triclosan resistant plasmid backbone was produced

When put together, our final maxicell product would therefore have virtually no risk to the biosphere upon environmental release.

Contact EdiGEM18

Feel free to leave us a comment on social media!