Difference between revisions of "Team:Uppsala/Demonstrate"
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| − | + | <div class="igem-icon"><a href="https://2018.igem.org/Team:Uppsala"><img src="https://static.igem.org/mediawiki/2018/c/cf/T--Uppsala--WormBusterLogo_Black.png"></a></div> | |
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| + | <!-- CONTENT OF WHATS ON THE PAGE --> | ||
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<div class ="content-text" id="scrolldown" > | <div class ="content-text" id="scrolldown" > | ||
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<div style="height:5em;"></div> | <div style="height:5em;"></div> | ||
<!-- FROM THIS POINT DOWNWARDS YOU START ADDING YOUR STUFF --> | <!-- FROM THIS POINT DOWNWARDS YOU START ADDING YOUR STUFF --> | ||
| − | + | <div class="card-holder"> | |
| − | <p>In the worm buster project we have divided our work into separate subgroups to handle the massive burden of the project. To read more about how we designed our experiments and the results we got click on the buttons below. They will take you to each page for the different parts of the wet-lab project | + | <p>In the worm buster project we have divided our work into separate subgroups to handle the massive burden of the project. To read more about how we designed our experiments and the results we got, click on the buttons below. They will take you to each page for the different parts of the wet-lab project.</p> |
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<!-------BUTTONS -------> | <!-------BUTTONS -------> | ||
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<div class="card-holder"> | <div class="card-holder"> | ||
| − | + | <div class="content-card content-card-2"> | |
| + | <div class="inner-card left-card"> | ||
| + | <h2>Transcriptomics</h2> | ||
| + | <br> | ||
| + | <div class="inner-card-text"> | ||
| + | <!-- start of paragraph--> | ||
| + | <p>We managed to perform our entire pipeline and sequence the <i>E.coli</i> transcriptome five times. Our result show that transcriptomics with MinION's Nanopore Technology works if a better technique to reduce the amount of RNA in the sample is discovered.</p> | ||
| + | </div> | ||
| + | <div class="buttons"> | ||
<div class="container-button"> | <div class="container-button"> | ||
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<a href="https://2018.igem.org/Team:Uppsala/Transcriptomics" class="btn">Transcriptomics </a> | <a href="https://2018.igem.org/Team:Uppsala/Transcriptomics" class="btn">Transcriptomics </a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="inner-card right-card"> | ||
| + | <h2>Reporter System</h2> | ||
| + | <br> | ||
| + | <div class="inner-card-text"> | ||
| + | <!-- start of paragraph --> | ||
| + | <p>We proved that GFP is visible in horse feces and could be used as a reporter. We also integrated a previous BioBrick of the UnaG chromoprotein for possible use as reporter in our diagnostic tool.</p> | ||
| + | <!-- End of paragraphs --> | ||
| + | </div> | ||
| + | <div class="buttons"> | ||
| + | <div class="container-button"> | ||
| + | <a href="https://2018.igem.org/Team:Uppsala/Reporter_System" class="btn">Reporter System</a> | ||
| + | </div> | ||
| + | </div> | ||
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<div class="card-holder"> | <div class="card-holder"> | ||
| − | <div class="card- | + | <div class="content-card content-card-2"> |
| − | <p> | + | <div class="inner-card left-card"> |
| − | + | <h2>Phage Display</h2> | |
| − | </p> | + | <br> |
| − | </div> | + | <div class="inner-card-text"> |
| − | + | <!-- start of paragraph--> | |
| + | <p>Computational analysis of our phage display peptides showed two especially promising peptides:</p> | ||
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| + | <br> | ||
| + | <p><strong>EF01122224: TPIFLPTPAQEH </strong></p> | ||
| + | <p><strong>EF01122218: FSPTQANTIHRW </strong></p> | ||
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| + | </div> | ||
| + | <div class="buttons"> | ||
<div class="container-button"> | <div class="container-button"> | ||
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<a href="https://2018.igem.org/Team:Uppsala/Phage_Display" class="btn">Phage Display</a> | <a href="https://2018.igem.org/Team:Uppsala/Phage_Display" class="btn">Phage Display</a> | ||
| + | </div> | ||
| + | </div> | ||
| − | <a href="https://2018.igem.org/Team:Uppsala/ | + | </div> |
| − | + | <div class="inner-card right-card"> | |
| + | <h2>Worm Culturing</h2> | ||
| + | <br> | ||
| + | <div class="inner-card-text"> | ||
| + | <!-- start of paragraph --> | ||
| + | <p>We successfully sterilized and co-cultured <i>cyathostominae</i> with <i>E.coli</i> as well as creating a microfluidic chip for easier separation of large and small strongyles</p> | ||
| + | <!-- End of paragraphs --> | ||
| + | </div> | ||
| + | <div class="buttons" > | ||
| + | <div class="container-button"> | ||
| + | <a href="https://2018.igem.org/Team:Uppsala/Worm_Culturing" class="btn">Worm Culturing </a> | ||
| + | </div> | ||
| + | </div> | ||
| − | + | </div> | |
| − | + | </div> | |
| + | </div> | ||
<br><br><br><br> | <br><br><br><br> | ||
<!------END BUTTONS-----> | <!------END BUTTONS-----> | ||
Latest revision as of 01:48, 18 October 2018
In the worm buster project we have divided our work into separate subgroups to handle the massive burden of the project. To read more about how we designed our experiments and the results we got, click on the buttons below. They will take you to each page for the different parts of the wet-lab project.
Transcriptomics
We managed to perform our entire pipeline and sequence the E.coli transcriptome five times. Our result show that transcriptomics with MinION's Nanopore Technology works if a better technique to reduce the amount of RNA in the sample is discovered.
Reporter System
We proved that GFP is visible in horse feces and could be used as a reporter. We also integrated a previous BioBrick of the UnaG chromoprotein for possible use as reporter in our diagnostic tool.
Phage Display
Computational analysis of our phage display peptides showed two especially promising peptides:
EF01122224: TPIFLPTPAQEH
EF01122218: FSPTQANTIHRW
Worm Culturing
We successfully sterilized and co-cultured cyathostominae with E.coli as well as creating a microfluidic chip for easier separation of large and small strongyles