Difference between revisions of "Team:UMaryland/Hardware"

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<div class="titleRegion" style="background-image: url(https://static.igem.org/mediawiki/2018/7/7b/T--UMaryland--PTNT4.png)">
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<div class="titleText">Hardware</div>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="subtitleText">PET NET - Maximizing PETase Activity</div>
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The PET Net is a biocatalytic flow reactor specifically designed to maximize the degradation efficiency of the PETase fusion protein.
 
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The system consists of a series of interconnected tubes, the inner surface of which are covered in bacterial cellulose. It is partially inspired by the digestive system, which has evolved for efficient degradation of solid material. A slurry of shredded PET plastic and the PETase fusion protein are pumped into the constantly circulating reactor. One of the subunits of the fusion protein is a cellulose binding domain. This semi-immobilizes the protein on to the bacterial cellulose which coats the inside of the tubes. Since the binding is not as strong as a covalent bond, the protein will be able to flow with the slurry. The combination of semi immobilization and free flowing protein will help increase the exposure of multiple surfaces of PET to the active sites of PETase. By increasing the number of interactions of the plastic and the protein the likelihood of a successful catalytic reaction increases. PET Net features a modular design that allows for rapid scalability. The reactor modules can be easily produced and quickly assembled which gives the system flexibility to be used in a variety of situations. The system is cell free, making it more safe to operate in non-laboratory settings while removing the interference of cells with the catalysis of degradation.
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source : Tamborini, L., Fernandes, P., Paradisi, F., & Molinari, F. (2018). Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification. Trends in Biotechnology, 36(1), 73-88. doi:10.1016/j.tibtech.2017.09.005
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<h1>Hardware</h1>
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<h3>Best Hardware Special Prize</h3>
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<p>iGEM is about making teams of students making synthetic biology projects. We encourage teams to work with parts and build biological devices in the lab. But we are inclusive and want all teams to work on many other types of problems in synbio. Robotic assembly, microfluidics, low cost equipment and measurement hardware are all areas ripe for innovation in synbio. </p>
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Teams who are interested in working with hardware as a side project are encouraged to apply for the hardware award.
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To compete for the <a href="https://2018.igem.org/Judging/Awards">Best Hardware prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>.
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<img src="https://static.igem.org/mediawiki/2018/a/a4/T--UMaryland--petnetpipes2.png" style="max-width: 100%" alt="Waluigi Time!">
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<img src="https://static.igem.org/mediawiki/2018/8/84/T--UMaryland--petnetconnector.png" style="max-width: 100%" alt="Waluigi Time!">
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You must also delete the message box on the top of this page to be eligible for this prize.
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<div class="imageBoxDescription">Figure 1 - Internal Schematics of the PET NET</div>
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<h3>Inspiration</h3>
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<img src="https://static.igem.org/mediawiki/2018/7/7c/T--UMaryland--petnet2.png" style="max-width: 100%" alt="Waluigi Time!">
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<p>You can look at what other teams did to get some inspiration! <br />
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<img src="https://static.igem.org/mediawiki/2018/1/1c/T--UMaryland--petnetii.png" style="max-width: 100%" alt="Waluigi Time!">
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Here are a few examples:</p>
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<div class="imageBoxDescription">Figure 2 - Model of Assembled PET NET</div>
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<li><a href="https://2016.igem.org/Team:Valencia_UPV">2016 Valencia UPV</a></li>
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<li><a href="https://2016.igem.org/Team:Aachen">2016 Aachen </a></li>
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<li><a href="https://2015.igem.org/Team:TU_Delft">2015 TU Delft  </a></li>
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<li><a href="https://2015.igem.org/Team:TU_Darmstadt">2015 TU Darmstadt</a></li>
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Latest revision as of 01:51, 18 October 2018

Template Title Template Title

Hardware
PET NET - Maximizing PETase Activity
The PET Net is a biocatalytic flow reactor specifically designed to maximize the degradation efficiency of the PETase fusion protein.
The system consists of a series of interconnected tubes, the inner surface of which are covered in bacterial cellulose. It is partially inspired by the digestive system, which has evolved for efficient degradation of solid material. A slurry of shredded PET plastic and the PETase fusion protein are pumped into the constantly circulating reactor. One of the subunits of the fusion protein is a cellulose binding domain. This semi-immobilizes the protein on to the bacterial cellulose which coats the inside of the tubes. Since the binding is not as strong as a covalent bond, the protein will be able to flow with the slurry. The combination of semi immobilization and free flowing protein will help increase the exposure of multiple surfaces of PET to the active sites of PETase. By increasing the number of interactions of the plastic and the protein the likelihood of a successful catalytic reaction increases. PET Net features a modular design that allows for rapid scalability. The reactor modules can be easily produced and quickly assembled which gives the system flexibility to be used in a variety of situations. The system is cell free, making it more safe to operate in non-laboratory settings while removing the interference of cells with the catalysis of degradation.
source : Tamborini, L., Fernandes, P., Paradisi, F., & Molinari, F. (2018). Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification. Trends in Biotechnology, 36(1), 73-88. doi:10.1016/j.tibtech.2017.09.005
Waluigi Time! Waluigi Time!
Figure 1 - Internal Schematics of the PET NET
Waluigi Time! Waluigi Time!
Figure 2 - Model of Assembled PET NET

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