Difference between revisions of "Team:FSU/Improve"

 
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<h1>Improve</h1>
 
<h1>Improve</h1>
  
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<p>Looking back at previous iGEM teams, Berkeley in 2008 uploaded several sequences that were hypothesized to contain promotors that were activated when an Escherichia coli cell was exposed to sound. As a characterization of previous parts, the FSU 2018 iGEM team is furthering Berkeley’s investigation to test these sequences and to figure out whether these sequences, naturally found in E. coli, hold a promotor and whether they respond to sound waves. After constructing the plasmids with each putative promotor and red fluorescent gene, the cells containing BBa_K112402 were expressing red before being exposed to sound. The Berkeley team believed that somewhere in this sequence was a promotor for the fabA gene, and after our initial results, it does seem as though there is a functioning constitutive promotor within this sequence. Our characterized test device with this promotor has been uploaded to the Part’s Registry as BBa_K2832011. We believe that this sequence will be enhanced with sound, but further investigations in our experiment will determine this and new data will be presented at the Jamboree.</p>
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<p><p>The 2008 U.C. Berkeley iGEM team uploaded several sequences expected to contain promotors activated when an Escherichia Coli cell was exposed to sound. As an improvement of previous iGEM parts, the FSU 2018 iGEM team further investigated Berkeley’s parts to test these sequences, which are found naturally in E. coli, hold a promotor that responds to sound stress. After we constructed five devices which include a Berkeley putative promotor and the sequence that codes for red fluorescent protein and assembled the parts into plasmids. The cells containing Berkeley promoter BBa_K112402 were expressing red before sound exposure. Experimentally, this promoter, which Berkeley believed that somewhere in this sequence was a promotor for the fabA gene, stimulates constitutive expression. Our characterized test device with this promotor has been uploaded to the Part’s Registry as BBa_K2832011. Our measurements are being analyzed to see if expression is upregulated when exposed to sound. These measurements will be presented at the Jamboree.</p>
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<img src="https://static.igem.org/mediawiki/2018/9/9e/T--FSU--placeholder3D.png">
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<p><img src="https://static.igem.org/mediawiki/parts/0/0e/T--BBa_K2832011.png" style="width:96%"><p/>
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<p>Plasmid map for the BBa_K2832011 test cells. Our device in this plasmid includes the Berkeley Promoter BBa_K112402 and the coding sequence for mRFP<p/>
  
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
 
  
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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<p><img src="https://static.igem.org/mediawiki/2018/3/3a/T--FSU--berkeley-promotor.png" style="width:96%"><p/>
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<p>This Diagram shows RFP expression in our test cells, which include our test device BBa_K2832011 before sound exposure.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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Latest revision as of 02:10, 18 October 2018

Improve

The 2008 U.C. Berkeley iGEM team uploaded several sequences expected to contain promotors activated when an Escherichia Coli cell was exposed to sound. As an improvement of previous iGEM parts, the FSU 2018 iGEM team further investigated Berkeley’s parts to test these sequences, which are found naturally in E. coli, hold a promotor that responds to sound stress. After we constructed five devices which include a Berkeley putative promotor and the sequence that codes for red fluorescent protein and assembled the parts into plasmids. The cells containing Berkeley promoter BBa_K112402 were expressing red before sound exposure. Experimentally, this promoter, which Berkeley believed that somewhere in this sequence was a promotor for the fabA gene, stimulates constitutive expression. Our characterized test device with this promotor has been uploaded to the Part’s Registry as BBa_K2832011. Our measurements are being analyzed to see if expression is upregulated when exposed to sound. These measurements will be presented at the Jamboree.

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Plasmid map for the BBa_K2832011 test cells. Our device in this plasmid includes the Berkeley Promoter BBa_K112402 and the coding sequence for mRFP

This Diagram shows RFP expression in our test cells, which include our test device BBa_K2832011 before sound exposure.