Difference between revisions of "Team:Georgia State"
| (69 intermediate revisions by 2 users not shown) | |||
| Line 50: | Line 50: | ||
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Bootstrap? | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Bootstrap? | ||
action=raw&ctype=text/css"> | action=raw&ctype=text/css"> | ||
| + | |||
<!-- Magnific Popup --> | <!-- Magnific Popup --> | ||
| Line 72: | Line 73: | ||
<body> | <body> | ||
| − | + | <div class="clear"> | |
<div id="page"> | <div id="page"> | ||
| Line 81: | Line 82: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-sm-2 col-xs-12"> | <div class="col-sm-2 col-xs-12"> | ||
| − | <div id="gtco-logo"><a href=" | + | <div id="gtco-logo"><a href="/Team:Georgia_State"><img src="wiki/images/1/12/T--Georgia_State--logosmall.png" alt=""></a></div> |
</div> | </div> | ||
<div class="col-xs-10 text-right menu-1"> | <div class="col-xs-10 text-right menu-1"> | ||
<ul> | <ul> | ||
<li class="active"><a href="/Team:Georgia_State">Home</a></li> | <li class="active"><a href="/Team:Georgia_State">Home</a></li> | ||
| − | |||
<li class="has-dropdown"> | <li class="has-dropdown"> | ||
| − | + | <a href="/Team:Georgia_State/Team">Team</a> | |
| + | <ul class="dropdown"> | ||
| + | <li><a href="/Team:Georgia_State/Attributions">Attributions</a></li> | ||
| + | <li><a href="/Team:Georgia_State/Collaborations">Collaborations</a></li> | ||
| + | |||
| + | </ul> | ||
| + | <li class="has-dropdown"> | ||
| + | <a href="/Team:Georgia_State">Project</a> | ||
<ul class="dropdown"> | <ul class="dropdown"> | ||
| − | |||
<li><a href="/Team:Georgia_State/Design">Design</a></li> | <li><a href="/Team:Georgia_State/Design">Design</a></li> | ||
| − | + | <li><a href="/Team:Georgia_State/Experiments">Experiments</a></li> | |
| − | <li><a href="/Team:Georgia_State/ | + | <li><a href="/Team:Georgia_State/Improve">Improve</a></li> |
| + | <li><a href="/Team:Georgia_State/Demonstrate">Demonstrate</a><li> | ||
| + | <li><a href="/Team:Georgia_State/Results">Results</a></li> | ||
| + | |||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="has-dropdown"> | + | <li><a href="/Team:Georgia_State/Notebook">Notebook</a></li> |
| − | + | <li> | |
| + | <a href="/Team:Georgia_State/Basic_Part">Parts</a> | ||
| + | </li> | ||
| + | <li><a href="/Team:Georgia_State/InterLab">InterLab</a></li> | ||
| + | <li><a href="/Team:Georgia_State/Safety">Safety</a></li> | ||
| + | <li class="has-dropdown"> | ||
| + | <a href="/Team:Georgia_State/Human_Practices">Human Practices</a> | ||
<ul class="dropdown"> | <ul class="dropdown"> | ||
| − | + | <li><a href="/Team:Georgia_State/Public_Engagement">Public Engagement</a></li> | |
| − | + | <li><a href="/Team:Georgia_State/ASL">ASL Gallery</a></li> | |
| − | + | </ul> | |
| − | + | </li> | |
| − | + | ||
| − | + | <li><a href="https://igem.org/2018_Judging_Form?team=Georgia_State">Judging Form</a></li> | |
| − | + | ||
| − | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 113: | Line 126: | ||
</div> | </div> | ||
| + | |||
</nav> | </nav> | ||
| + | |||
<header id="gtco-header" class="gtco-cover" role="banner"> | <header id="gtco-header" class="gtco-cover" role="banner"> | ||
| Line 127: | Line 142: | ||
<div class="col-md-7 copy"> | <div class="col-md-7 copy"> | ||
<h1> Georgia State University</h1> | <h1> Georgia State University</h1> | ||
| − | <p> | + | <p>STEM Accessible to All</p> |
</div> | </div> | ||
| Line 143: | Line 158: | ||
<div class="gtco-container"> | <div class="gtco-container"> | ||
<div class="row row-pb-sm"> | <div class="row row-pb-sm"> | ||
| − | <div class="col-md- | + | <div class="col-md-12 col-md-offset-0 gtco-heading text-center"> |
<h2>Is Your Detector Expecting? See with HCG!</h2> | <h2>Is Your Detector Expecting? See with HCG!</h2> | ||
<p> | <p> | ||
Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.</p> | Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.</p> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <!--Begin Footer--> | ||
<footer id="gtco-footer" class="gtco-section" role="contentinfo"> | <footer id="gtco-footer" class="gtco-section" role="contentinfo"> | ||
<div class="gtco-container"> | <div class="gtco-container"> | ||
| Line 174: | Line 194: | ||
<br> | <br> | ||
<br> | <br> | ||
| − | <li> <img src="https://static.igem.org/mediawiki/2015/5/5f/GSUiGEM-2015Twitter.png" width= "55px"class="pull-left" alt=""> | + | <li><img src="https://static.igem.org/mediawiki/2015/5/5f/GSUiGEM-2015Twitter.png" width= "55px"class="pull-left" alt=""> |
<a href="https://twitter.com/GSUiGEM" target="_blank">GSUiGEM</a></li> | <a href="https://twitter.com/GSUiGEM" target="_blank">GSUiGEM</a></li> | ||
| Line 196: | Line 216: | ||
</div> | </div> | ||
<div class="col-md-6 text-right"> | <div class="col-md-6 text-right"> | ||
| − | <p><small>Based on a design by <a href="http://gettemplates.co/" target="_blank">GetTemplates.co</a | + | <p><small>Based on a design by <a href="http://gettemplates.co/" target="_blank">GetTemplates.co</a></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 202: | Line 222: | ||
</div> | </div> | ||
</footer> | </footer> | ||
| − | |||
| − | |||
<div class="gototop js-top"> | <div class="gototop js-top"> | ||
| − | <a href="#" class="js-gotop">< | + | <a href="#" class="js-gotop"><span class="glyphicon" style="font-size:20px"></span></a> |
</div> | </div> | ||
| − | + | </div> | |
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 02:11, 18 October 2018
Georgia State University
STEM Accessible to All
Is Your Detector Expecting? See with HCG!
Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.