Difference between revisions of "Team:UMaryland/Parts"

 
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825001">BBa_K2825001</a>
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PETase-CBD-HlyA
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The part contains isPETase linked to a cellulose binding domain linked to a HlyA secretion tag.
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PcaU Sensor
 
PcaU Sensor
 
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Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation
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Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor.
 
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<th>Registry Number</th>
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<th>Part Name</th>
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<td>BBa_K2825000</td>
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<td>PETase-Linker-CBDcipA-Linker-HlyA tag + HlyBD secretion system</td>
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<td>The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase, and a cellulose binding domain.</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002">http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002</a></td>
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<td>BBa_K2825002</td>
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<td>PcaU Sensor</td>
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<td>Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor.</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002">http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002</a></td>
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Latest revision as of 02:21, 18 October 2018

Template Title Template Title

Parts
Our Contribution to the Registry
BBa_K2825000
PETase-Linker-CBDcipA-Linker-HlyA tag + HlyBD secretion system

The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase (), and a cellulose binding domain.
BBa_K2825002
PcaU Sensor

Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor.
Parts Table
Registry Number Part Name Description Link
BBa_K2825000 PETase-Linker-CBDcipA-Linker-HlyA tag + HlyBD secretion system The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase, and a cellulose binding domain. http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002
BBa_K2825002 PcaU Sensor Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor. http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002

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