Difference between revisions of "Team:Oxford/notebook"

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<h1 class="header-text">Overview</h1>
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                    <h1 class="header-text2">Notebook</h1>
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            <h2>Wet Lab</h2>
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<p>A key part of our project has been our work in the Wet Lab. This page contains links and summaries of all the aspects of lab work we did.</p>  
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<p><b>Results</b></p>
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<p>The first port of call for any lab work is to clone the DNA for our constructs into pSB1C3. This is the simple cloning vector with Chloramphenicol resistance chosen by iGEM to be the shipping vector for all parts to be submitted in. After this, the vectors would be transformed into the ideal cells optimised for the particular experiments they were undergoing. <br>
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All the protocols we used during the duration of iGEM can be found below. <br>
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How we used these protocols in different experiments can be found below.<br>
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<p> We used the website 'Benchling' to note down all the experiments we performed in the lab. This made sure a summary of the most recent lab work was easily accessible to all members of the team. We split our lab notebook into two files; one recorded work on "Digestion, SDM, Ligation and Microbiology' and the other was used to note down 'PCR and oePCR' reactions. </p>
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        <a data-toggle="collapse" href="#notebook1">Digestion, SDM, Ligation and Microbiology</a>
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<p><b>Wet Lab day to day working </b></p>
 
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<p>We only had a few months in the lab and made a lot of progress but there’s much more that could have been done. See our day to day workings here as well as what we’d have in store to take the project on after the jamboree .</p>
 
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<p><b> Interlab </b></p>
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<p>This year we participated in the 5th instalment of the iGEM’s interlab study. For the details pertaining to what interlab is and what we did - click on the link below. </p>
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        <a data-toggle="collapse" href="#notebook2">PCR and oePCR</a>
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<p> To best visualise images of the gels we ran, the notebook can be viewed using this <a href="https://benchling.com/s/etr-XxwQsDETQswP3D4Dax26" style="text-decoration:underline;">link.</a> </p>
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Latest revision as of 02:33, 18 October 2018

Full Width Pics - Start Bootstrap Template

Notebook

We used the website 'Benchling' to note down all the experiments we performed in the lab. This made sure a summary of the most recent lab work was easily accessible to all members of the team. We split our lab notebook into two files; one recorded work on "Digestion, SDM, Ligation and Microbiology' and the other was used to note down 'PCR and oePCR' reactions.

Digestion, SDM, Ligation and Microbiology

PCR and oePCR

To best visualise images of the gels we ran, the notebook can be viewed using this link.