Difference between revisions of "Team:DTU-Denmark/Parts"

 
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Line 4: Line 4:
 
{{DTU-Denmark/Experimentscss}}
 
{{DTU-Denmark/Experimentscss}}
 
<html>
 
<html>
−
<div style="position: relative; top: 0; right: 0;z-index: 0;width:100%; left: 0;opacity: 1; overflow: hidden;
 
−
    padding: 0;">
 
−
<img src="https://static.igem.org/mediawiki/2018/7/7a/T--DTU-Denmark--notebooktestbackground.png" style="max-width: 100%; filter: brightness(85%); " >
 
−
<div class="h1" style="position: absolute;
 
−
    top: 30%;
 
−
    left: 50%;
 
  
−
transform: translate(-50%, -50%);
+
<div class="toppercontainer">
−
    color:#fff;font-size:100px;">Parts</div>
+
<img src="https://static.igem.org/mediawiki/2018/4/44/T--DTU-Denmark--topperparts.png" style="max-width: 100%; object-fit: cover; width:100%;" >
 +
<div class="headlinecontainer"><h1>Parts</h1></div>
 
</div>
 
</div>
 +
  
 
<div class="igem_2018_team_content">
 
<div class="igem_2018_team_content">
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<div class="interlabspace">
 
<div class="interlabspace">
−
<H2 style="text-align: left;margin-bottom:20px;">Mycolab protocols</H2>
+
<H2 style="text-align: left;margin-bottom:20px;"></H2>
  
  
 
<!--#############################################################################################
 
<!--#############################################################################################
−
MelA
+
melA
 
##############################################################################################-->
 
##############################################################################################-->
  
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799000"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799000</button></a><button class="collapsiblebig">MelA</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799000"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799000</button></a><button class="collapsiblebig"><i>melA</i></button>
  
  
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick is the coding region of MelA that has been codon-optimized to Aspergillus oryzae by DTU Biobuilders 2018. The protein sequence is based on a previous BioBrick.</p>
+
<p>This BioBrick contains the coding region of<i> melA</i> codon optimized for <i>Aspergillus oryzae</i> by the DTU Biobuilders 2018. The optimization was performed on <a target="_blank" href="http://parts.igem.org/Part:BBa_K193600">BBa_K193600</a> using the software tool, <a target="_blank" href="https://2016.igem.org/Team:DTU-Denmark/Software">TaiCO</a>, developed by the team in 2016.</p>
 
</div>
 
</div>
  
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</div>
 
</div>
 
<!--#############################################################################################
 
<!--#############################################################################################
−
Nat1
+
nat1
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799001"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799001</button></a><button class="collapsiblebig">Nat1</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799001"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799001</button></a><button class="collapsiblebig"><i>nat1</i></button>
  
  
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick is the coding region for the Nat1 gene, that provides resistance to NCT in filamentous fungi.</p>
+
<p>This BioBrick contains the coding region of the <i>nat1 gene</i> encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in <i>Aspergillus oryzae</i>.</p>
 
</div>
 
</div>
  
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<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">pGpdA - AmilCP - tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">PgpdA - AmilCP - tCaMV</button>
  
  
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>
 +
This BioBrick contains the chromoprotein AmilCP for expression in fungi. It has the glyceraldehyde-3-phosphate dehydrogenase promoter, P<i>gpdA</i>, derived from <i>Aspergillus nidulans</i> and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 
</div>
 
</div>
  
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<!--#############################################################################################
 
<!--#############################################################################################
−
pTrpC - Hph- tCaMV
+
PtrpC - hph - tCaMV
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799013</button></a><button class="collapsiblebig"> pTrpC - Hph- tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799013</button></a><button class="collapsiblebig"> PtrpC - hph - tCaMV</button>
  
  
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the resistence gene Hph for expression in fungi. It has the promoter pTrpC and terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>This BioBrick encompasses the gene <i>hph</i>, which confers resistance to Hygromycin B, for expression in fungi. It has the <i>Aspergillus nidulans</i> promoter P<i>trpC</i> and terminator from the Cauliflower Mosaic Virus (CaMV).
 +
.</p>
 
</div>
 
</div>
  
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<!--#############################################################################################
 
<!--#############################################################################################
−
pTrpC - MelA - tCaMV
+
PtrpC - melA - tCaMV
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799014</button></a><button class="collapsiblebig">pTrpC - MelA - tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799014</button></a><button class="collapsiblebig">PtrpC - melA - tCaMV</button>
  
  
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the MelA gene for expression in fungi. It has the promoter pTrpC and terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>This BioBrick functions to increase expression of melanin in fungi. It contains <i>melA</i>, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter P<i>trpC</i> derived from <i>Aspergillus nidulans</i>. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV).
 +
</p>
 
</div>
 
</div>
  
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−
<!-- TOOOOOOOOOOOOOOOOOO HERE-->
+
 
  
 
<!--#############################################################################################
 
<!--#############################################################################################
−
pGpdA - AmilCP - tCaMV
+
pCaMV - melA - tCaMV
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">pGpdA - AmilCP - tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799015</button></a><button class="collapsiblebig">pCaMV - melA - tCaMV</button>
  
  
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   </svg>
 
   </svg>
 
</headerparts><headerparts2>
 
</headerparts><headerparts2>
−
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1623</h3>
+
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">2451</h3>
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>This BioBrick encompasses the <i>melA</i> gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 
</div>
 
</div>
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/47/T--DTU-Denmark--parts4.png" style="max-width: 60%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--DTU-Denmark--parts7.png" style="max-width: 60%;"   
 
</p>
 
</p>
  
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<!--#############################################################################################
 
<!--#############################################################################################
−
pGpdA - AmilCP - tCaMV
+
Hph selection marker + chromoprotein
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">pGpdA - AmilCP - tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799021</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button>
  
  
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   </svg>
 
   </svg>
 
</headerparts><headerparts2>
 
</headerparts><headerparts2>
−
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1623</h3>
+
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">2924</h3>
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p>
 
</div>
 
</div>
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/47/T--DTU-Denmark--parts4.png" style="max-width: 60%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/b/b6/T--DTU-Denmark--parts8.png" style="max-width: 100%;"   
 
</p>
 
</p>
  
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−
 
+
<!-- TOOOOOOOOOOOOOOOOOO HERE-->
  
  
 
<!--#############################################################################################
 
<!--#############################################################################################
−
pGpdA - AmilCP - tCaMV
+
Hph selection marker + chromoprotein
 
##############################################################################################-->
 
##############################################################################################-->
  
 
<div class="buttonshadow">
 
<div class="buttonshadow">
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">pGpdA - AmilCP - tCaMV</button>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799022</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button>
  
  
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   </svg>
 
   </svg>
 
</headerparts><headerparts2>
 
</headerparts><headerparts2>
−
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1623</h3>
+
   <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">3257</h3>
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
   <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
 
     <polygon fill="white" points="0,100 0,0 100,100"/>
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</headerparts2>
 
</headerparts2>
 
<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
+
<p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p>
 
</div>
 
</div>
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/47/T--DTU-Denmark--parts4.png" style="max-width: 60%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/0/06/T--DTU-Denmark--parts9.png" style="max-width: 60%;"   
 
</p>
 
</p>
  
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−
 
−
 
−
 
−
<!--#############################################################################################
 
−
pGpdA - AmilCP - tCaMV
 
−
##############################################################################################-->
 
−
 
−
<div class="buttonshadow">
 
−
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">pGpdA - AmilCP - tCaMV</button>
 
−
 
−
 
−
<div class="contentparts">
 
−
<headerparts>
 
−
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 
−
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
−
    <polygon fill="white" points="0,100 100,0 100,100"/>
 
−
  </svg>
 
−
</headerparts><headerparts2>
 
−
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1623</h3>
 
−
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 
−
    <polygon fill="white" points="0,100 0,0 100,100"/>
 
−
  </svg>
 
−
</headerparts2>
 
−
<div class="sizemargins">
 
−
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has promoter pGdpA and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 
−
</div>
 
−
 
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/47/T--DTU-Denmark--parts4.png" style="max-width: 60%;" 
 
−
</p>
 
−
 
−
</div>
 
−
 
−
</div>
 
  
  
Line 421: Line 387:
 
<a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a>
 
<a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a>
 
&bull;
 
&bull;
−
                       <a href="https://2018.igem.org/Team:DTU-Denmark/Model">Modelling</a>
+
                       <a href="https://2018.igem.org/Team:DTU-Denmark/Model">Modeling</a>
 
&bull;
 
&bull;
 
<a href="https://2018.igem.org/Team:DTU-Denmark/Parts">Parts overview</a>
 
<a href="https://2018.igem.org/Team:DTU-Denmark/Parts">Parts overview</a>

Latest revision as of 02:37, 18 October 2018

Parts

Type

Coding

Length

1830

This BioBrick contains the coding region of melA codon optimized for Aspergillus oryzae by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.

Type

Coding

Length

573

This BioBrick contains the coding region of the nat1 gene encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in Aspergillus oryzae.

Type

Composite

Length

1290

This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1623

This BioBrick contains the chromoprotein AmilCP for expression in fungi. It has the glyceraldehyde-3-phosphate dehydrogenase promoter, PgpdA, derived from Aspergillus nidulans and the terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1626

This BioBrick encompasses the gene hph, which confers resistance to Hygromycin B, for expression in fungi. It has the Aspergillus nidulans promoter PtrpC and terminator from the Cauliflower Mosaic Virus (CaMV). .

Type

Composite

Length

2430

This BioBrick functions to increase expression of melanin in fungi. It contains melA, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter PtrpC derived from Aspergillus nidulans. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2451

This BioBrick encompasses the melA gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2924

This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.

Type

Composite

Length

3257

This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.