Difference between revisions of "Team:DTU-Denmark/Parts"
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</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick contains the coding region of<i> melA</i> codon optimized for <i>Aspergillus oryzae</i> by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.</p> | + | <p>This BioBrick contains the coding region of<i> melA</i> codon optimized for <i>Aspergillus oryzae</i> by the DTU Biobuilders 2018. The optimization was performed on <a target="_blank" href="http://parts.igem.org/Part:BBa_K193600">BBa_K193600</a> using the software tool, <a target="_blank" href="https://2016.igem.org/Team:DTU-Denmark/Software">TaiCO</a>, developed by the team in 2016.</p> |
</div> | </div> | ||
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</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick contains the coding region of the <i>nat1 gene</i> encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin. The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in <i>Aspergillus oryzae</i>.</p> | + | <p>This BioBrick contains the coding region of the <i>nat1 gene</i> encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in <i>Aspergillus oryzae</i>.</p> |
</div> | </div> | ||
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<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799011"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799011</button></a><button class="collapsiblebig"> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799011"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799011</button></a><button class="collapsiblebig">pCaMV - AmilCP - tCaMV</button> |
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<!--############################################################################################# | <!--############################################################################################# | ||
| − | + | PtrpC - hph - tCaMV | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799013</button></a><button class="collapsiblebig"> PtrpC - | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799013</button></a><button class="collapsiblebig"> PtrpC - hph - tCaMV</button> |
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</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick encompasses the | + | <p>This BioBrick encompasses the gene <i>hph</i>, which confers resistance to Hygromycin B, for expression in fungi. It has the <i>Aspergillus nidulans</i> promoter P<i>trpC</i> and terminator from the Cauliflower Mosaic Virus (CaMV). |
| + | .</p> | ||
</div> | </div> | ||
| Line 187: | Line 188: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | + | PtrpC - melA - tCaMV | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799014</button></a><button class="collapsiblebig"> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799014</button></a><button class="collapsiblebig">PtrpC - melA - tCaMV</button> |
| Line 207: | Line 208: | ||
</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick functions to increase expression of melanin in fungi. It contains <i>melA</i>, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter | + | <p>This BioBrick functions to increase expression of melanin in fungi. It contains <i>melA</i>, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter P<i>trpC</i> derived from <i>Aspergillus nidulans</i>. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV). |
</p> | </p> | ||
</div> | </div> | ||
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<!--############################################################################################# | <!--############################################################################################# | ||
| − | pCaMV - | + | pCaMV - melA - tCaMV |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799015</button></a><button class="collapsiblebig">pCaMV - | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799015</button></a><button class="collapsiblebig">pCaMV - melA - tCaMV</button> |
| Line 243: | Line 244: | ||
</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick encompasses the | + | <p>This BioBrick encompasses the <i>melA</i> gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).</p> |
</div> | </div> | ||
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<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799021</button></a><button class="collapsiblebig"> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799021</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button> |
| Line 277: | Line 278: | ||
</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick contains the | + | <p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p> |
</div> | </div> | ||
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<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799022</button></a><button class="collapsiblebig"> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799022</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button> |
| Line 313: | Line 314: | ||
</headerparts2> | </headerparts2> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This BioBrick contains the | + | <p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p> |
</div> | </div> | ||
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<a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a> | <a href="https://2018.igem.org/Team:DTU-Denmark/Description">Project description</a> | ||
• | • | ||
| − | <a href="https://2018.igem.org/Team:DTU-Denmark/Model"> | + | <a href="https://2018.igem.org/Team:DTU-Denmark/Model">Modeling</a> |
• | • | ||
<a href="https://2018.igem.org/Team:DTU-Denmark/Parts">Parts overview</a> | <a href="https://2018.igem.org/Team:DTU-Denmark/Parts">Parts overview</a> | ||
Latest revision as of 02:37, 18 October 2018
Parts
Type
Coding
Length
1830
This BioBrick contains the coding region of melA codon optimized for Aspergillus oryzae by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.
Type
Coding
Length
573
This BioBrick contains the coding region of the nat1 gene encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in Aspergillus oryzae.
Type
Composite
Length
1290
This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).
Type
Composite
Length
1623
This BioBrick contains the chromoprotein AmilCP for expression in fungi. It has the glyceraldehyde-3-phosphate dehydrogenase promoter, PgpdA, derived from Aspergillus nidulans and the terminator from the Cauliflower Mosaic Virus (CaMV).
Type
Composite
Length
1626
This BioBrick encompasses the gene hph, which confers resistance to Hygromycin B, for expression in fungi. It has the Aspergillus nidulans promoter PtrpC and terminator from the Cauliflower Mosaic Virus (CaMV). .
Type
Composite
Length
2430
This BioBrick functions to increase expression of melanin in fungi. It contains melA, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter PtrpC derived from Aspergillus nidulans. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV).
Type
Composite
Length
2451
This BioBrick encompasses the melA gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).
