Difference between revisions of "Team:DTU-Denmark/Parts"

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{{DTU-Denmark}}
 
{{DTU-Denmark}}
 +
{{DTU-Denmark/Styling}}
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{{DTU-Denmark/footercss}}
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{{DTU-Denmark/Experimentscss}}
 
<html>
 
<html>
  
 +
<div class="toppercontainer">
 +
<img src="https://static.igem.org/mediawiki/2018/4/44/T--DTU-Denmark--topperparts.png" style="max-width: 100%; object-fit: cover; width:100%;" >
 +
<div class="headlinecontainer"><h1>Parts</h1></div>
 +
</div>
  
−
<div class="column full_size">
+
 
−
<h1>Parts</h1>
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<div class="igem_2018_team_content">
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
<div class="igem_2018_team_column_wrapper">
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
<div class="container-fluid" style="width: 80%;margin-left: auto;
 +
    margin-right: auto;position:relative;margin-top:0%;">
 +
<div class="row">
 +
 
 +
 
 +
 
 +
<div class="interlabspace">
 +
<H2 style="text-align: left;margin-bottom:20px;"></H2>
 +
 
 +
 
 +
<!--#############################################################################################
 +
melA
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799000"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799000</button></a><button class="collapsiblebig"><i>melA</i></button>
 +
 
 +
 
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Coding</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1830</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick contains the coding region of<i> melA</i> codon optimized for <i>Aspergillus oryzae</i> by the DTU Biobuilders 2018. The optimization was performed on <a target="_blank" href="http://parts.igem.org/Part:BBa_K193600">BBa_K193600</a> using the software tool, <a target="_blank" href="https://2016.igem.org/Team:DTU-Denmark/Software">TaiCO</a>, developed by the team in 2016.</p>
 
</div>
 
</div>
  
−
<div class="column full_size">
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/45/T--DTU-Denmark--part1-melA.png" style="max-width: 100%;" 
−
<div class="highlight decoration_background">
+
</p>
−
<h3>Note</h3>
+
 
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
 
</div>
 
</div>
 
</div>
 
</div>
 +
<!--#############################################################################################
 +
nat1
 +
##############################################################################################-->
  
−
<div class="clear extra_space"></div>
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<div class="buttonshadow">
−
<div class="line_divider"></div>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799001"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799001</button></a><button class="collapsiblebig"><i>nat1</i></button>
−
<div class="clear extra_space"></div>
+
  
  
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Coding</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">573</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick contains the coding region of the <i>nat1 gene</i> encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in <i>Aspergillus oryzae</i>.</p>
 +
</div>
  
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/5/51/T--DTU-Denmark--parts-nat1.png" style="max-width: 100%;" 
 +
</p>
  
 +
</div>
 +
</div>
  
−
<div class="column two_thirds_size">
+
<!--#############################################################################################
−
<div class="highlight decoration_B_full">
+
pCaMV - AmilCP - tCaMV
 +
##############################################################################################-->
  
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<h3>Adding parts to the registry</h3>
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<div class="buttonshadow">
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799011"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799011</button></a><button class="collapsiblebig">pCaMV - AmilCP - tCaMV</button>
  
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
 
−
<div class="button_link">
+
<div class="contentparts">
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
<headerparts>
−
ADD PARTS
+
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
−
</a>
+
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1290</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 
</div>
 
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/1/1e/T--DTU-Denmark--parts3.png" style="max-width: 60%;" 
 +
</p>
  
 
</div>
 
</div>
 +
 
</div>
 
</div>
  
  
 +
<!--#############################################################################################
 +
pGpdA - AmilCP - tCaMV
 +
##############################################################################################-->
  
−
<div class="column third_size">
+
<div class="buttonshadow">
−
<div class="highlight decoration_A_full">
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799012</button></a><button class="collapsiblebig">PgpdA - AmilCP - tCaMV</button>
−
<h3>Inspiration</h3>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
 
−
<ul>
+
<div class="contentparts">
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
<headerparts>
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
−
</ul>
+
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1623</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>
 +
This BioBrick contains the chromoprotein AmilCP for expression in fungi. It has the glyceraldehyde-3-phosphate dehydrogenase promoter, P<i>gpdA</i>, derived from <i>Aspergillus nidulans</i> and the terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 
</div>
 
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/4/47/T--DTU-Denmark--parts4.png" style="max-width: 60%;" 
 +
</p>
 +
 
</div>
 
</div>
  
 +
</div>
  
−
<div class="clear extra_space"></div>
 
  
  
  
  
−
<div class="column full_size">
 
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
<!--#############################################################################################
−
<p>The information needed to initially create a part on the Registry is:</p>
+
PtrpC - hph - tCaMV
−
<ul>
+
##############################################################################################-->
−
<li>Part Name</li>
+
 
−
<li>Part type</li>
+
<div class="buttonshadow">
−
<li>Creator</li>
+
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799013</button></a><button class="collapsiblebig"> PtrpC - hph - tCaMV</button>
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
 
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">1626</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick encompasses the gene <i>hph</i>, which confers resistance to Hygromycin B, for expression in fungi. It has the <i>Aspergillus nidulans</i> promoter P<i>trpC</i> and terminator from the Cauliflower Mosaic Virus (CaMV).
 +
.</p>
 
</div>
 
</div>
  
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/2/26/T--DTU-Denmark--parts5.png" style="max-width: 60%;" 
 +
</p>
  
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
 +
<!--#############################################################################################
 +
PtrpC - melA - tCaMV
 +
##############################################################################################-->
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799014</button></a><button class="collapsiblebig">PtrpC - melA - tCaMV</button>
 +
 +
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">2430</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick functions to increase expression of melanin in fungi. It contains <i>melA</i>, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter P<i>trpC</i> derived from <i>Aspergillus nidulans</i>. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV).
 +
</p>
 +
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/c/cf/T--DTU-Denmark--parts6.png" style="max-width: 60%;" 
 +
</p>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
 +
 +
<!--#############################################################################################
 +
pCaMV - melA - tCaMV
 +
##############################################################################################-->
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799015</button></a><button class="collapsiblebig">pCaMV - melA - tCaMV</button>
 +
 +
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">2451</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick encompasses the <i>melA</i> gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).</p>
 +
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--DTU-Denmark--parts7.png" style="max-width: 60%;" 
 +
</p>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
 +
<!--#############################################################################################
 +
Hph selection marker + chromoprotein
 +
##############################################################################################-->
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799021</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button>
 +
 +
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">2924</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p>
 +
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/b/b6/T--DTU-Denmark--parts8.png" style="max-width: 100%;" 
 +
</p>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
<!-- TOOOOOOOOOOOOOOOOOO HERE-->
 +
 +
 +
<!--#############################################################################################
 +
Hph selection marker + chromoprotein
 +
##############################################################################################-->
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2799022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K2799022</button></a><button class="collapsiblebig">hph selection marker + chromoprotein</button>
 +
 +
 +
<div class="contentparts">
 +
<headerparts>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Type</h3> <h3 style="color: white;text-align:center;font-size:250%;">Composite</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 100,0 100,100"/>
 +
  </svg>
 +
</headerparts><headerparts2>
 +
  <h3 style="color: white;text-align:center;padding: 50px 0 0 0 ;">Length</h3> <h3 style="color: white;text-align:center;font-size:250%;">3257</h3>
 +
  <svg viewBox="0 0 100 100" preserveAspectRatio="none">
 +
    <polygon fill="white" points="0,100 0,0 100,100"/>
 +
  </svg>
 +
</headerparts2>
 +
<div class="sizemargins">
 +
<p>This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.</p>
 +
</div>
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2018/0/06/T--DTU-Denmark--parts9.png" style="max-width: 60%;" 
 +
</p>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<script>
 +
var coll = document.getElementsByClassName("collapsiblebig");
 +
var i;
 +
 +
for (i = 0; i < coll.length; i++) {
 +
  coll[i].addEventListener("click", function() {
 +
 +
    this.classList.toggle("coactivebleu");
 +
    var content = this.nextElementSibling;
 +
    if (content.style.maxHeight){
 +
      content.style.maxHeight = null;
 +
    } else {
 +
      content.style.maxHeight = content.scrollHeight + "px";
 +
    }
 +
  });
 +
}
 +
</script>
 +
 +
 +
<!--************************************************************
 +
***************    FOOTER    ***********************************
 +
**************************************************************-->
 +
 +
<div class="interlabspace">
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
−
<div class="line_divider"></div>
+
 
 +
</div>
 +
<div class="interlabspace"><div class="clear extra_space"></div>
 +
 
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
  
−
<div class="column full_size">
+
</div>
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<h3>Part Table </h3>
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Latest revision as of 02:37, 18 October 2018

Parts

Type

Coding

Length

1830

This BioBrick contains the coding region of melA codon optimized for Aspergillus oryzae by the DTU Biobuilders 2018. The optimization was performed on BBa_K193600 using the software tool, TaiCO, developed by the team in 2016.

Type

Coding

Length

573

This BioBrick contains the coding region of the nat1 gene encoding a nourseothricin acetyltransferase, which confers resistance to nourseothricin (NTC). The sequence was kindly provided by Jakob Rendsvig and is codon-optimized for expression in Aspergillus oryzae.

Type

Composite

Length

1290

This BioBrick encompasses the chromoprotein AmilCP for expression in fungi. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1623

This BioBrick contains the chromoprotein AmilCP for expression in fungi. It has the glyceraldehyde-3-phosphate dehydrogenase promoter, PgpdA, derived from Aspergillus nidulans and the terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

1626

This BioBrick encompasses the gene hph, which confers resistance to Hygromycin B, for expression in fungi. It has the Aspergillus nidulans promoter PtrpC and terminator from the Cauliflower Mosaic Virus (CaMV). .

Type

Composite

Length

2430

This BioBrick functions to increase expression of melanin in fungi. It contains melA, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter PtrpC derived from Aspergillus nidulans. Its terminator is derived from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2451

This BioBrick encompasses the melA gene for expression in fungi/eukaryotes, thus increasing expression of melanin. It has the promoter and terminator from the Cauliflower Mosaic Virus (CaMV).

Type

Composite

Length

2924

This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.

Type

Composite

Length

3257

This BioBrick contains the hph selection marker and the chromoprotein AmilCP for expression in fungi.