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<h2>Key results from Optimization and Modeling</h2>
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<li>Decreasing net charge on the peptide improves expression efficiency, however it decreases its antibacterial activity</li>
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Revision as of 03:03, 18 October 2018

Key Results

Key results from the Human Practices

  • By talking to experts, farmers and veterinaries, we identified that antimicrobial resistance of coliforms affecting piglets is a major concern
  • We obtained access to raw data from ANSES and found out that this data highlights amoxicillin and tetracycline as drugs with the most frequent evolved resistance

    • Key results from the STAR CORE Design and Production

  • We successfully cloned 131 STAR CORE combinations.
  • 9 out of the 11 proteins we expressed exhibited antibacterial activity comparable to existing antibiotics and AMPs.

    • Key results from the Testing

  • The STAR CORE we have obtained show antimicrobial activity.
  • 8 out of the 10 constructs showed antimicrobial activity towards E. coli while 9 out of the 10 constructs displayed antimicrobial potential towards B. subtilis.
  • The top performing STAR CORE was the Ferritin-Alyteserin fusion, with an activity almost 10 times higher than that of the control. Some StarCores exhibited species-specific killing, being particularly active against E. coli.
  • StarCores of varying geometry displayed similar MIC values.
  • There was no clear correlation between the geometry of the STAR CORE and their antimicrobial activity, indicating a non-specific mechanism of action.

    • Key results from Optimization and Modeling

  • Decreasing net charge on the peptide improves expression efficiency, however it decreases its antibacterial activity
    • Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    paris-bettencourt-2018@cri-paris.org