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| | + | {{SSHS-Shenzhen/CSS}} |
| | | | |
| | <html lang="en"> | | <html lang="en"> |
| Line 4: |
Line 5: |
| | <meta charset="UTF-8"> | | <meta charset="UTF-8"> |
| | <title>Title</title> | | <title>Title</title> |
| − | </head>
| + | <style> |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <style > | + | |
| − | /*解决上边和右边的东西*/
| + | |
| − | #globalWrapper{
| + | |
| − | margin: 0;
| + | |
| − | padding: 0;
| + | |
| − | }
| + | |
| − | #content{
| + | |
| − | width: 100%;
| + | |
| − | padding: 0;
| + | |
| − | margin: -12px 0 0;
| + | |
| − | }
| + | |
| − | #top_title{
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | #sideMenu{
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | #content,
| + | |
| − | .vs-content,
| + | |
| − | .vs-wrapper section {
| + | |
| − | background-color: transparent !important;
| + | |
| − | }
| + | |
| − | .my-backTop:visited {
| + | |
| − | color: #F8A886 !important;
| + | |
| − | }
| + | |
| − | /*解决上边和右边的东西*/
| + | |
| − | body{background-color:#E8F1CB;
| + | |
| − | }
| + | |
| − | .header {
| + | |
| − | font-size: 30px;
| + | |
| − | padding:0px;
| + | |
| − | margin:0px;
| + | |
| − | top:0;
| + | |
| − | left:0;
| + | |
| − | width:100%;
| + | |
| − | position: fixed;
| + | |
| − | height:3em;
| + | |
| − | z-index:99;
| + | |
| − | float: left;
| + | |
| − | background-color: #FFFFFF;
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | p{
| + | |
| − | padding:5px;}
| + | |
| − | h4{text-align:center;}
| + | |
| − | ul li
| + | |
| − | {
| + | |
| − | list-style:none;
| + | |
| − | margin:15px;
| + | |
| − | padding-top:6px;
| + | |
| − | padding-bottom:6px;
| + | |
| − | padding-left: 0;
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | li a,.dropbtn,a:link,a:visited{
| + | |
| − | text-decoration: none;
| + | |
| − | font-family: "Arial";
| + | |
| − | color: #3C3C3C;
| + | |
| − | padding:20px;
| + | |
| − | text-align:center;
| + | |
| − | font-size: 20px;
| + | |
| − | }
| + | |
| − | li a:hover,.dropdown:hover.dropbtn{
| + | |
| − | color: #07cf65;
| + | |
| − | text-shadow:2px 2px 2px #c5f484;
| + | |
| − | }
| + | |
| − | | + | |
| − | .dropdown {
| + | |
| − | position: relative;
| + | |
| − | display: inline-block;
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | .dropdown-content {
| + | |
| − | display: none;
| + | |
| − | position: absolute;
| + | |
| − | background-color: #FFFFFF;
| + | |
| − | min-width: 300px;
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .dropdown-content a {
| + | |
| − | color: #3C3C3C;
| + | |
| − | padding: 12px 16px;
| + | |
| − | text-decoration: none;
| + | |
| − | display: block;
| + | |
| − | font-size: 20px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .dropdown-content a:hover {
| + | |
| − | background-color: transparent;}
| + | |
| − | | + | |
| − | .dropdown:hover .dropdown-content {
| + | |
| − | display: block;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | #page{
| + | |
| − | text-align:justify;
| + | |
| − | padding-left:170px;
| + | |
| − | padding-right:170px;
| + | |
| − | padding-top:80px;
| + | |
| − | padding-bottom:100px;
| + | |
| − | line-height:25px;
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | #teamtext{
| + | |
| − | text-align:justify;
| + | |
| − | padding-left:170px;
| + | |
| − | padding-right:50px;
| + | |
| − | padding-top:80px;
| + | |
| − | padding-bottom:100px;
| + | |
| − | line-height:25px;
| + | |
| − | | + | |
| − | | + | |
| − | }
| + | |
| − | #teamphoto{
| + | |
| − | text-align:justify;
| + | |
| − | padding-left:50px;
| + | |
| − | padding-right:170px;
| + | |
| − | padding-top:80px;
| + | |
| − | padding-bottom:100px;
| + | |
| − | line-height:25px;
| + | |
| − | | + | |
| − | | + | |
| − | }
| + | |
| − | #teamall{
| + | |
| − | display: flex;
| + | |
| − | flex-direction: row;
| + | |
| − | column-count: 2;
| + | |
| − | | + | |
| − | }
| + | |
| − | | + | |
| − | #sec1{
| + | |
| − | width: auto;
| + | |
| − | background-color:#e8f1cb;
| + | |
| − | }
| + | |
| − | #sec2{
| + | |
| − | width: auto;
| + | |
| − | background-color:#d0e8c1;
| + | |
| − | }
| + | |
| − | #sec3{
| + | |
| − | width: auto;
| + | |
| − | background-color:#ffffff;
| + | |
| − | }
| + | |
| − | | + | |
| | | | |
| | + | h1{ |
| | + | color: #fff; |
| | + | padding:100px 50px 5px!important; |
| | + | font-size: 30px!important; |
| | + | text-align: center; |
| | + | } |
| | + | body { |
| | + | background-color: #000;} |
| | </style> | | </style> |
| | + | </head> |
| | <body> | | <body> |
| | + | <div style="height:90px"> </div> |
| | + | <center> |
| | | | |
| − | <div class="header"> | + | <img src= "https://static.igem.org/mediawiki/2018/1/10/T--SSHS-Shenzhen--%E6%A1%8C%E9%9D%A23.jpg" width="100%"> |
| − | <ul>
| + | <div style="height:60px"> </div> |
| − | <li>
| + | |
| − | <div class="dropdown">
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen">Home</a>
| + | |
| − | </div>
| + | |
| | | | |
| − | | + | <div style="height:30px"> </div> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Description" class="dropbtn">Project</a>
| + | <video width=70% controls> |
| − | <div class="dropdown-content">
| + | <source src="https://static.igem.org/mediawiki/2018/4/46/T--SSHS-Shenzhen--video2.mp4" type="video/mp4"> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Description">Description</a>
| + | </video></center> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Applied_Design">Applied Design</a>
| + | <h1> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Model">Model</a>
| + | A Battle against the Beetle |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Demonstrate">Demonstrate</a>
| + | </h1> |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="dropdown">
| + | <p id="para"> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Experiments" class="dropbtn">Experiments</a>
| + | Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations, for example, chemical insecticide applications will cause dietary pollution and environmental destruction. Here, we aim to develop an RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The effect of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provides an environmentally friendly approach for insect control. |
| − | <div class="dropdown-content">
| + | </p> |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Experiments">Summary</a>
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Results">Results</a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="dropdown">
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Parts" >Parts</a>
| + | |
| − | <div class="dropdown-content">
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Parts">Summary</a>
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Basic_Part">Basic Parts</a>
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Composite_Part">Composite Parts</a>
| + | |
| − | <a href="https://2018.igem.org/Team:SMS_Shenzhen/Contribution">Contribution</a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="dropdown">
| + | <center> |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Collaborations">Collaborations</a>
| + | <div style=" width:1200px; height:400px"> |
| − | </div>
| + | <div style=" float:left; width:600px;line-height: 400px"><center><img src=" |
| − | | + | https://static.igem.org/mediawiki/2018/6/67/T--SSHS-Shenzhen--%E5%9B%A2%E9%98%9F%E5%9B%BE%E6%A0%87.jpg |
| − | <div class="dropdown">
| + | " width=80%></center></div> |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Human_Practices" class="dropbtn">Human Practice</a>
| + | <div style=" float:left; width:600px;line-height: 400px"><center><img src=" |
| − | <div class="dropdown-content">
| + | https://static.igem.org/mediawiki/2018/3/36/T--SSHS-Shenzhen--%E5%AD%A6%E6%A0%A1%E5%9B%BE%E6%A0%87.jpg |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Human_Practices">Summary</a>
| + | " width=80%></center></div> |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/HP/Silver">Silver</a>
| + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/HP/Gold_Integrated">Gold</a>
| + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Engagement">Engagement</a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="dropdown">
| + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Safety" class="dropbtn">Safety</a>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Team" class="dropbtn">Team</a>
| + | |
| − | <div class="dropdown-content">
| + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Team">Team Members</a>
| + | |
| − | <a href="https://2017.igem.org/Team:SMS_Shenzhen/Attributions">Attributions</a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <!--照抄前年的代码了-->
| + | |
| − | | + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| | </div> | | </div> |
| | + | </center> |
| | | | |
| − | <body> | + | <br><br><br><br><br> |
| − | | + | |
| − | <div id="sec1"> | + | |
| − | <div id="page">
| + | |
| − | <center>
| + | |
| − | <h2>Project Description</h2>
| + | |
| − | </center>
| + | |
| − | <p style="font-size:18px">This year, SMS_Shenzhen team will focus on using synthetic biologic method to prevent dental plaque. Dental plaque can be led by Streptococcus mutans, a bacteria lives in human's mouth. Clinging to the teeth in thin layers called biofilm, S. mutans digests sucrose and produces acids that can eat into enamel and cause cavities. Specifically, dextran is the main component of the biofilm. We find two enzymes, the first one is named "Dextranase", which can hydrolyze the dextran in the biofilm; and the second one is named "FruA", which can decompose the resource that S. mutans uses to produce bioflim. The gene of these two enzymes are cloned into E. coli and Lactobacillus. In our experiment, we would use E.coli to produce these two enzymes for relative measurement like enzyme activity. Then, for commercial design, we would produce leben with our Lactobacillus which can secrete these two enzymes.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| | </body> | | </body> |
| | </html> | | </html> |
Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations, for example, chemical insecticide applications will cause dietary pollution and environmental destruction. Here, we aim to develop an RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The effect of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provides an environmentally friendly approach for insect control.
