Difference between revisions of "Team:SSHS-Shenzhen"

 
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Phyllotreta striolata killers
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A Battle against the Beetle
 
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Phyllotreta striolata is the most destructive insects worldwide.<br><br>However, the present insect control strategies have certain limitations.<br><br>We aim to develop a RNAi-based topical application approach.<br><br>Ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNA silencing mechanism in the P. striolata and lead to its death.<br><br>So far, we have designed the siRNAs for synthesis based on the sequences of the genes that are important for the development and survival of P. striolata.<br><br>Currently we are rearing P. striolata to obtain enough the same age insects for feeding bioassays and gene silencing investigation.<br><br>We will design corresponding small hairpin RNAs (shRNAs) and use E. coli expression system, or in vitro transcription system to produce these shRNAs in large scale.
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Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations, for example, chemical insecticide applications will cause dietary pollution and environmental destruction. Here, we aim to develop an RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The effect of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provides an environmentally friendly approach for insect control.
 
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Latest revision as of 03:21, 18 October 2018

Title

Title

A Battle against the Beetle

Phyllotreta striolata is one of the most destructive insects worldwide. However, the present insect control strategies have certain limitations, for example, chemical insecticide applications will cause dietary pollution and environmental destruction. Here, we aim to develop an RNAi-based approach for controlling P. striolata. This approach is to topically apply exogenous siRNAs/shRNAs onto vegetables, ingestion of the sprayed siRNAs or shRNAs by P. striolata will induce the RNAi mechanism in the insect and lead to its death. In our project, siRNAs/shRNAs were designed based on the mRNA sequences of their target genes. The effect of both siRNAs and shRNAs in mediating RNAi in P. striolata were examined. Experimental results show that both siRNAs and shRNA could successfully silence their target genes, which was demonstrated by the survival rate decrease after siRNA or shRNA treatment. Our project provides an environmentally friendly approach for insect control.