Difference between revisions of "Team:DTU-Denmark/Safety"

 
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<h1> Safety </h1>
 
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
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<div class="headlinecontainer"><h1>Safety</h1></div>
 
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h3>Safe Shipment</h3>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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<h2 class="media-heading" style="text-align: left;margin-bottom: 35px; color:#50C8E8;">Choosing a non-pathogenic chassis</h2>
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<p style="text-align:justify" >The <i>Aspergillus</i> family consists of a few hundred of mold species that prosper in a large number of different climates around the world. They can have various applications ranging from microbial fermentations to medicinal applications. However, it has been reported that some of the strains can cause animal and human diseases (1). The choice of <i>Aspergillus oryzae</i> as our experimental organism was vastly dependent on its safety features. Briefly, invasive growth or systemic infections by <i>A. oryzae</i> in healthy humans have never been reported. In addition to this, <i>A. oryzae</i> does not produce aflatoxins or any other cancerogenic metabolites. In spite of its low pathogenic potential <i>A. oryzae</i> may like other harmless microorganisms, grow in human tissues under exceptional circumstances. For example, allergic diseases primarily caused by <i>A. oryzae</i> have been reported in a few cases, but their occurrence is probably dependent on the sensitivity of the diseased individual (2). Therefore, we consider <i>A. oryzae</i> as an excellent host for our experiments.
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<h2 class="media-heading"  style="text-align: right;margin-bottom: 35px; color:#F8A05B;">Drylab's unusual experiments</h2>
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Testing the fungal material in the lab can give rise to various unusual safety concerns. One of them is that we had to be absolutely sure that the resulting GMO material was completely dead after the completion of the experiments, preventing undesired occurrences such as the spread of living fungal spores. As indicated by the literature (3), baking of the products and testing their viability afterward could resolve this issue. Specifically, spores needed to be killed at 70&deg; Celsius(3) during an overnight incubation, which we confirmed experimentally. In addition to this, we exposed our fungi to large amounts of gamma radiation (comparable to a dose rate received on Mars). These experiments were performed in a controlled environment at DTU Risø, where the sources were encapsulated in lead, and only raised after the testing room was evacuated. The walls of the testing room were lined with lead, and dosimeters were worn at all times to check the levels of background radiation. In addition to this, we exposed our fungi to large amounts of gamma radiation (comparable to a dose rate received on Mars). These experiments were performed in a controlled environment at DTU Risø, where the sources were encapsulated in lead, and only raised after the testing room was evacuated.
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There is really not much to say about the safety procedures used every day in the lab at DTU Byg, which have all the big machines used for testing the mechanical properties of the fungi. We have to be sure to NOT stick our appendages (hands, fingers etc.) into the machine performing the compression tests. It moves very slowly, so it is in principle hard to have any accidents there.
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<p style="color:#000; font-size:14px;">(1) Microbe wiki. 2018. Aspergillus oryzae.<br><br>
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(2) Barbesgaard P, Heldt-Hansen H, Diderichsen B. 1992. Appl Microbiol Biotechnol 36:569-572.<br><br>
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(3) Jensen H. 1948. Thermal Death Points for Spores and Mycelia of Moulds on Fermented Tobacco. Physiol Plant 1:255-264. </p>
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Latest revision as of 03:22, 18 October 2018

Safety

Choosing a non-pathogenic chassis

The Aspergillus family consists of a few hundred of mold species that prosper in a large number of different climates around the world. They can have various applications ranging from microbial fermentations to medicinal applications. However, it has been reported that some of the strains can cause animal and human diseases (1). The choice of Aspergillus oryzae as our experimental organism was vastly dependent on its safety features. Briefly, invasive growth or systemic infections by A. oryzae in healthy humans have never been reported. In addition to this, A. oryzae does not produce aflatoxins or any other cancerogenic metabolites. In spite of its low pathogenic potential A. oryzae may like other harmless microorganisms, grow in human tissues under exceptional circumstances. For example, allergic diseases primarily caused by A. oryzae have been reported in a few cases, but their occurrence is probably dependent on the sensitivity of the diseased individual (2). Therefore, we consider A. oryzae as an excellent host for our experiments.

Drylab's unusual experiments

Testing the fungal material in the lab can give rise to various unusual safety concerns. One of them is that we had to be absolutely sure that the resulting GMO material was completely dead after the completion of the experiments, preventing undesired occurrences such as the spread of living fungal spores. As indicated by the literature (3), baking of the products and testing their viability afterward could resolve this issue. Specifically, spores needed to be killed at 70° Celsius(3) during an overnight incubation, which we confirmed experimentally. In addition to this, we exposed our fungi to large amounts of gamma radiation (comparable to a dose rate received on Mars). These experiments were performed in a controlled environment at DTU Risø, where the sources were encapsulated in lead, and only raised after the testing room was evacuated. The walls of the testing room were lined with lead, and dosimeters were worn at all times to check the levels of background radiation. In addition to this, we exposed our fungi to large amounts of gamma radiation (comparable to a dose rate received on Mars). These experiments were performed in a controlled environment at DTU Risø, where the sources were encapsulated in lead, and only raised after the testing room was evacuated.

There is really not much to say about the safety procedures used every day in the lab at DTU Byg, which have all the big machines used for testing the mechanical properties of the fungi. We have to be sure to NOT stick our appendages (hands, fingers etc.) into the machine performing the compression tests. It moves very slowly, so it is in principle hard to have any accidents there.

(1) Microbe wiki. 2018. Aspergillus oryzae.

(2) Barbesgaard P, Heldt-Hansen H, Diderichsen B. 1992. Appl Microbiol Biotechnol 36:569-572.

(3) Jensen H. 1948. Thermal Death Points for Spores and Mycelia of Moulds on Fermented Tobacco. Physiol Plant 1:255-264.