Difference between revisions of "Team:Munich/assembly3.html"

 
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<h4>T7 and 3S Phage Assembly in self-produced cell-extract </h4
+
<h4>T7 and 3S Phage Assembly in Self-Produced Cell-Extract </h4>
 
<em>2018/09/21- 2018/09/22</em>
 
<em>2018/09/21- 2018/09/22</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
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     <tr>
 
     <tr>
 
       <td>Notes:</td>
 
       <td>Notes:</td>
−
       <td> T7 DNA:  ca. 500 ng, <br>
+
       <td> T7 DNA:  ca. 500 ng <br>
 
3S Phage: 330 ng <br>
 
3S Phage: 330 ng <br>
 
Self-produced extract: E10
 
Self-produced extract: E10
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−
<h4>T7 Phage Assembly in self-produced cell-extract </h4
+
<h4>T7 Phage Assembly in Self-Produced Cell-Extract </h4>
 
<em>2018/09/21- 2018/09/22</em>
 
<em>2018/09/21- 2018/09/22</em>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
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     </tr>
 
     </tr>
 
</table>
 
</table>
 +
 +
<h4>
 +
Reproduction of T7 Phage Assembly in E10 and P15
 +
</h4>
 +
<em>
 +
2018/10/03-2018/10/05
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Sophie Schönberg, Brigit Tunaj</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX/TL</a>, 
 +
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay </a>
 +
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
        <td>
 +
No notes.
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
E 10: 2,1*10^8 PFU/mL <br>
 +
P15:5,1*10^6 PFU/mL<br>
 +
Lyophilized P15: 1*10^5 PFU/mL<br>
 +
 +
</td>
 +
</tr>
 +
</table>
 +
 +
 +
<h4>
 +
Reproduction of T7 Phage Assembly in E10 and P15
 +
</h4>
 +
<em>
 +
2018/10/07-2018/10/08
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Sophie Schönberg, Brigit Tunaj</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX/TL</a>, 
 +
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay </a>
 +
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
        <td>
 +
No notes.
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
E 10 lyophilized: 1,4*10^6 PFU/mL <br>
 +
P15:3,2*10^6 PFU/mL<br>
 +
Lyophilized P15: 1,5*10^6 PFU/mL<br>
 +
 +
</td>
 +
</tr>
 +
</table>
 +
 +
 +
<h4>
 +
Reproduction of T7 Phage Assembly in E10 and P15
 +
</h4>
 +
<em>
 +
2018/10/07-2018/10/08
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Sophie Schönberg, Brigit Tunaj</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX/TL</a>, 
 +
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay </a>
 +
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
        <td>
 +
No notes.
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
E 10 lyophilized 2,7*10^6 PFU/mL.
 +
</td>
 +
</tr>
 +
</table>
 +
 +
 +
<h4>
 +
Assembly of various Phages in commercial cell extract
 +
</h4>
 +
<em>
 +
2018/10/10-2018/10/11
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Brigit Tunaj</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX/TL</a>, 
 +
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay </a>
 +
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
        <td>
 +
Phages: T5,T4,T7,3S, P2,P3
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
Titer: <br>
 +
T5: 6*10^7 PFU/mL<br>
 +
T7: 1,8*10^8 PFU/mL<br>
 +
T4: 4*10^9 PFU/mL<br>
 +
3S:5*^06 PFU/mL<br>
 +
P3:3*10^7 PFU/mL<br>
 +
P2:2,1*10^9 PFU/mL<br>
 +
 +
</td>
 +
</tr>
 +
</table>
 +
 +
 +
<h4>
 +
Engineered MS2 Phage in self produced cell extract P15
 +
</h4>
 +
<em>
 +
2018/10/10
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Brigit Tunaj</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage T7 Assembly TX/TL</a>, 
 +
<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay </a>
 +
 +
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
        <td>
 +
No notes.
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
3*10^7 PFU/mL
 +
</td>
 +
</tr>
 +
</table>
 +
 +
<h4>
 +
TEM picture of various phages
 +
</h4>
 +
<em>
 +
2018/10/10 -2018/10/17
 +
</em>
 +
<table class=”table table-borderless”>
 +
<tr>
 +
<td>Participants:</td>
 +
<td>Brigit Tunaj, with supervisor Kilian Vogele</td>
 +
</tr>
 +
<tr>
 +
<td>Protocol:</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2018/2/29/T--Munich--Protocol_TEM_preparation.pdf" target="_blank">TEM preparation</a>
 +
</td>
 +
</tr>
 +
<td>Notes:</td>
 +
      <td>
 +
No notes.
 +
      </td>
 +
</tr>
 +
<td>Results:</td>
 +
<td>
 +
Pictures are shown in results part
 +
</td>
 +
</tr>
 +
</table>
 +
 +
 
  </div>
 
  </div>
 
</html>
 
</html>

Latest revision as of 03:39, 18 October 2018

T7 and 3S Phage Assembly in Self-Produced Cell-Extract

2018/09/21- 2018/09/22
Participants: Brigit Tunaj, Sophie Schönberg von Schönberg
Protocol: Phage T7 Assembly TX-TL, Agar overlay plaque assay
Notes: T7 DNA: ca. 500 ng
3S Phage: 330 ng
Self-produced extract: E10
Results: T7 Phage: 1,6*10^8 PFU/mL
3S: did not work out. Decided to redo the experiment.

T7 Phage Assembly in Self-Produced Cell-Extract

2018/09/21- 2018/09/22
Participants: Brigit Tunaj, Sophie Schönberg von Schönberg
Protocol: Phage T7 Assembly TX-TL, Agar overlay plaque assay
Notes: T7 DNA: ca. 500 ng,
Results: T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL

Reproduction of T7 Phage Assembly in E10 and P15

2018/10/03-2018/10/05
Participants: Sophie Schönberg, Brigit Tunaj
Protocol: Phage T7 Assembly TX/TL, Agar overlay plaque assay
Notes: No notes.
Results: E 10: 2,1*10^8 PFU/mL
P15:5,1*10^6 PFU/mL
Lyophilized P15: 1*10^5 PFU/mL

Reproduction of T7 Phage Assembly in E10 and P15

2018/10/07-2018/10/08
Participants: Sophie Schönberg, Brigit Tunaj
Protocol: Phage T7 Assembly TX/TL, Agar overlay plaque assay
Notes: No notes.
Results: E 10 lyophilized: 1,4*10^6 PFU/mL
P15:3,2*10^6 PFU/mL
Lyophilized P15: 1,5*10^6 PFU/mL

Reproduction of T7 Phage Assembly in E10 and P15

2018/10/07-2018/10/08
Participants: Sophie Schönberg, Brigit Tunaj
Protocol: Phage T7 Assembly TX/TL, Agar overlay plaque assay
Notes: No notes.
Results: E 10 lyophilized 2,7*10^6 PFU/mL.

Assembly of various Phages in commercial cell extract

2018/10/10-2018/10/11
Participants: Brigit Tunaj
Protocol: Phage T7 Assembly TX/TL, Agar overlay plaque assay
Notes: Phages: T5,T4,T7,3S, P2,P3
Results: Titer:
T5: 6*10^7 PFU/mL
T7: 1,8*10^8 PFU/mL
T4: 4*10^9 PFU/mL
3S:5*^06 PFU/mL
P3:3*10^7 PFU/mL
P2:2,1*10^9 PFU/mL

Engineered MS2 Phage in self produced cell extract P15

2018/10/10
Participants: Brigit Tunaj
Protocol: Phage T7 Assembly TX/TL, Agar overlay plaque assay
Notes: No notes.
Results: 3*10^7 PFU/mL

TEM picture of various phages

2018/10/10 -2018/10/17
Participants: Brigit Tunaj, with supervisor Kilian Vogele
Protocol: TEM preparation
Notes: No notes.
Results: Pictures are shown in results part