Difference between revisions of "Team:UMaryland/InterLab"

 
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<div class="row"> <!-- honestly idk why this is here - i just copied it from somewhere and then now i'm too scared to remove this class !-->
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<div class="titleRegion" style="background-image: url(https://static.igem.org/mediawiki/2018/6/66/T--UMaryland--patternAlternateTwo.png)">
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<div class="container" style="height: 200px;">
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<div class="containerTitle">
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<div class="titleText">InterLab</div>
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<div class="TitleText">Interlab Study</div>
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<div class="SubtitleText">UMaryland's Interlab Study!</div>
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<!-- i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way i regret doing it this way !-->
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<div class="columnLeft c66"> <!-- Where the actual content actually is !-->
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<div id="Overview">
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<div id="introduction">
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Synthetic biology is all about collaboration between scientists in an attempt to further our understanding of the field and improve the world around us. However, with the variability that occurs in synthetic biology measurement, it is much harder to consistently build on work done by others. This year, the 5th Interlab Study was designed to reduce lab to lab variability in fluorescence by normalizing data to Colony Forming Units (CFU). The 2018 UMaryland iGEM team has performed the interlab study using the <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf"><u>protocol</u></a> distributed by the Measurement Committee.
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<p>We did this year's iGEM InterLab study. It was tremendous.</p>
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<br><br>
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<p>We followed the protocol given by iGEM absolutely perfectly. Look at the beautiful protocol we followed <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">here</a>.</p>
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Feel free to download our data <a href="https://static.igem.org/mediawiki/2018/e/ec/T--UMaryland--interlab_excel_sheet.xlsx"><u>here</u></a>.
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<p>Download our excel file we actually used <a href="https://static.igem.org/mediawiki/2018/e/ec/T--UMaryland--interlab_excel_sheet.xlsx">here</a>.</p>
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</div>
 
</div>
 
</div>
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<div id="Calibration">
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<div id="procedures">
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<h2>Calibration</h2>
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<div class="meatSubtitle">
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<h3>OD600 Reference Point - LUDOX Protocol</h3>
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Procedures
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<table id="calibrationTable">
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</div>
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<tr>
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<div class="meatMeat">
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<th></th>
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All measurements were performed using a standard clear 96-well plate. Calibration of our microplate reader used LUDOX CL-X as a single point reference to appropriately convert absorbance values into a standard OD600 measurement. Absorbances were measured for four replicates per LUDOX and deionized water at 600 nm. The particle standard curve was produced using silica beads and measured at 600 nm. There was also a fluorescence standard curve produced by using fluorescein and 1xPBS solution. Once all calibrations were completed, transformations of all devices in DH5-α, including positive and negative controls took place. Two colonies were chosen from each and incubated in 5 mL of LB media with chloramphenicol. They were left overnight for 16 hours at 37 C at around 220 rpm. All samples were immediately placed on ice and 100 ul was pipetted into the 96 well plate at the 0 and 6h timepoints. The absorbance and fluorescence was measured for both. The CFU measurements were taken by diluting the overnight cultures to an OD of 0.1. The samples were then diluted 1:20 three times and then 1:10 another two times. The final three dilutions for each replicate were plated and colonies were counted after 18 hours of growth at 37 C to determine the CFU/ml as can be seen in our results section.
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<th>LUDOX CL-X</th>
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</div>
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<th>H2O</th>
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</tr>
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<tr>
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<th>Replicate 1</th>
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−
<td class="blueCells">0.058</td>
+
−
<td class="blueCells">0.034</td>
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−
</tr>
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−
<tr>
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<th>Replicate 2</th>
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<td class="blueCells">0.056</td>
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<td class="blueCells">0.035</td>
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−
</tr>
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−
<tr>
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−
<th>Replicate 3</th>
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<td class="blueCells">0.058</td>
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−
<td class="blueCells">0.035</td>
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−
</tr>
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−
<tr>
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−
<th>Replicate 4</th>
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<td class="blueCells">0.058</td>
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−
<td class="blueCells">0.035</td>
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−
</tr>
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−
<tr>
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−
<th>Arithmetic Mean</th>
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<td class="yellowCells">0.058</td>
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−
<td class="yellowCells">0.035</td>
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−
</tr>
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<tr>
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<th>Corrected Abs600</th>
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<td class="yellowCells">0.058</td>
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<td></td>
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</tr>
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<tr>
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<th>Reference OD600</th>
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<td class="yellowCells">0.058</td>
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−
<td></td>
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</tr>
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<tr>
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−
<th>OD600/Abs600</th>
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−
<td class="yellowCells">0.058</td>
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<td></td>
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</tr>
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</table>
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<h3>Particle Standard Curve - Microsphere Protocol</h3>
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<table id="microsphereTable">
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<tr>
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<th>Number of Particles</th>
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<th>2.35×10<sup>8</sup></th>
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<th>1.18×10<sup>8</sup></th>
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−
<th>5.88×10<sup>7</sup></th>
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−
<th>2.94×10<sup>7</sup></th>
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−
<th>1.47×10<sup>7</sup></th>
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−
<th>7.35×10<sup>6</sup></th>
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−
<th>3.68×10<sup>6</sup></th>
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−
<th>1.84×10<sup>6</sup></th>
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−
<th>9.19×10<sup>5</sup></th>
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−
<th>4.60×10<sup>5</sup></th>
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−
<th>2.30×10<sup>5</sup></th>
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<th>0</th>
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−
</tr>
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−
<tr class="blueCells">
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−
<th class="whiteCellsImportant">Replicate 1</th>
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−
<td>0.716</td>
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−
<td>0.322</td>
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−
<td>0.191</td>
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−
<td>0.095</td>
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−
<td>0.067</td>
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−
<td>0.059</td>
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−
<td>0.039</td>
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−
<td>0.036</td>
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−
<td>0.036</td>
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−
<td>0.035</td>
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−
<td>0.034</td>
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−
<td>0.036</td>
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−
</tr>
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−
<tr class="blueCells">
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−
<th class="whiteCellsImportant">Replicate 2</th>
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−
<td>0.406</td>
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−
<td>0.359</td>
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−
<td>0.212</td>
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−
<td>0.111</td>
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−
<td>0.072</td>
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−
<td>0.051</td>
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−
<td>0.044</td>
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−
<td>0.036</td>
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−
<td>0.036</td>
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−
<td>0.035</td>
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−
<td>0.034</td>
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−
<td>0.034</td>
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−
</tr>
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−
<tr class="blueCells">
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−
<th class="whiteCellsImportant">Replicate 3</th>
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−
<td>0.587</td>
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−
<td>0.347</td>
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−
<td>0.203</td>
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−
<td>0.116</td>
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−
<td>0.075</td>
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−
<td>-</td>
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−
<td>0.041</td>
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−
<td>0.038</td>
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−
<td>0.038</td>
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−
<td>0.036</td>
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−
<td>0.035</td>
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−
<td>0.034</td>
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−
</tr>
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−
<tr class="blueCells">
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−
<th class="whiteCellsImportant">Replicate 4</th>
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−
<td>0.815</td>
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−
<td>0.419</td>
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−
<td>0.219</td>
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−
<td>0.123</td>
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−
<td>0.081</td>
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−
<td>0.051</td>
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−
<td>0.048</td>
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−
<td>0.040</td>
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−
<td>0.038</td>
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−
<td>0.037</td>
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−
<td>0.036</td>
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−
<td>0.035</td>
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−
</tr>
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−
<tr class="yellowCells">
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−
<th class="whiteCellsImportant">Arithmetic Mean</th>
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−
<td>0.631</td>
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−
<td>0.364</td>
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−
<td>0.206</td>
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−
<td>0.111</td>
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−
<td>0.074</td>
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−
<td>0.054</td>
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−
<td>0.043</td>
+
−
<td>0.038</td>
+
−
<td>0.037</td>
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−
<td>0.036</td>
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−
<td>0.035</td>
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−
<td>0.035</td>
+
−
</tr>
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−
<tr class="yellowCells">
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−
<th class="whiteCellsImportant">Arithmetic Standard Deviation</th>
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−
<td>0.177</td>
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−
<td>0.038</td>
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−
<td>0.012</td>
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−
<td>0.012</td>
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−
<td>0.006</td>
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−
<td>0.005</td>
+
−
<td>0.004</td>
+
−
<td>0.002</td>
+
−
<td>0.001</td>
+
−
<td>0.001</td>
+
−
<td>0.001</td>
+
−
<td>0.001</td>
+
−
</tr>
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−
<tr class="yellowCells">
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−
<th class="whiteCellsImportant">Arithmetic Net Mean</th>
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−
<td>0.596</td>
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−
<td>0.329</td>
+
−
<td>0.172</td>
+
−
<td>0.077</td>
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−
<td>0.039</td>
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−
<td>0.019</td>
+
−
<td>0.008</td>
+
−
<td>0.003</td>
+
−
<td>0.002</td>
+
−
<td>0.001</td>
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−
<td>0.000</td>
+
−
<td class="whiteCellsImportant"></td>
+
−
</tr>
+
−
</table>
+
−
<img src="https://static.igem.org/mediawiki/2018/b/bb/T--UMaryland--interlab_particle_standard_curve.png" alt="interlab particle standard curve">
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−
<img src="https://static.igem.org/mediawiki/2018/7/77/T--UMaryland--interlab_particle_standard_curve_log.png" alt="interlab particle standard curve">
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−
<table id="particlesPerOD">
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−
<tr>
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−
<th>Number of Particles</th>
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−
<th>2.35×10<sup>8</sup></th>
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−
<th>1.18×10<sup>8</sup></th>
+
−
<th>5.88×10<sup>7</sup></th>
+
−
<th>2.94×10<sup>7</sup></th>
+
−
<th>1.47×10<sup>7</sup></th>
+
−
<th>7.35×10<sup>6</sup></th>
+
−
<th>3.68×10<sup>6</sup></th>
+
−
<th>1.84×10<sup>6</sup></th>
+
−
<th>9.19×10<sup>5</sup></th>
+
−
<th>4.60×10<sup>5</sup></th>
+
−
<th>2.30×10<sup>5</sup></th>
+
−
</tr>
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−
<tr class="yellowCells">
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−
<th class="whiteCellsImportant">µM Fluorescein/a.u.</th>
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−
<td>3.95×10<sup>8</sup></td>
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−
<td>3.57×10<sup>8</sup></td>
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−
<td>3.43×10<sup>8</sup></td>
+
−
<td>3.84×10<sup>8</sup></td>
+
−
<td>3.77×10<sup>8</sup></td>
+
−
<td>3.89×10<sup>8</sup></td>
+
−
<td>4.46×10<sup>8</sup></td>
+
−
<td>6.68×10<sup>8</sup></td>
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−
<td>4.80×10<sup>8</sup></td>
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−
<td>4.60×10<sup>8</sup></td>
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−
<td>-</td>
+
−
</tr>
+
−
<tr>
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−
<th>MEFL/a.u.</th>
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−
<td></td>
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−
<td class="yellowCells">3.70×10<sup>8</sup></td>
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−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
</tr>
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−
</table>
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−
<h3>Fluorescence Standard Curve - Fluorescein Protocol</h3>
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−
<table id="fluoresceinTable">
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−
<tr>
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−
<th>Fluorescein µM</th>
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−
<th>10</th>
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−
<th>5</th>
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−
<th>2.5</th>
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−
<th>1.25</th>
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−
<th>0.625</th>
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−
<th>0.313</th>
+
−
<th>0.156</th>
+
−
<th>0.078</th>
+
−
<th>0.039</th>
+
−
<th>0.0195</th>
+
−
<th>0.0098</th>
+
−
<th>0</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Replicate 1</th>
+
−
<td>27845</td>
+
−
<td>21611</td>
+
−
<td>15480</td>
+
−
<td>10321</td>
+
−
<td>5979</td>
+
−
<td>3376</td>
+
−
<td>1806</td>
+
−
<td>891</td>
+
−
<td>442</td>
+
−
<td>244</td>
+
−
<td>122</td>
+
−
<td>2</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Replicate 2</th>
+
−
<td>27711</td>
+
−
<td>19390</td>
+
−
<td>16110</td>
+
−
<td>10742</td>
+
−
<td>6223</td>
+
−
<td>3240</td>
+
−
<td>1756</td>
+
−
<td>895</td>
+
−
<td>467</td>
+
−
<td>227</td>
+
−
<td>115</td>
+
−
<td>2</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Replicate 3</th>
+
−
<td>27686</td>
+
−
<td>21709</td>
+
−
<td>15590</td>
+
−
<td>9458</td>
+
−
<td>5304</td>
+
−
<td>2972</td>
+
−
<td>1350</td>
+
−
<td>711</td>
+
−
<td>481</td>
+
−
<td>101</td>
+
−
<td>160</td>
+
−
<td>3</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Replicate 4</th>
+
−
<td>27431</td>
+
−
<td>21442</td>
+
−
<td>15379</td>
+
−
<td>9474</td>
+
−
<td>5366</td>
+
−
<td>2703</td>
+
−
<td>1427</td>
+
−
<td>716</td>
+
−
<td>359</td>
+
−
<td>197</td>
+
−
<td>115</td>
+
−
<td>3</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Arithmetic Mean</th>
+
−
<td>27668</td>
+
−
<td>21038</td>
+
−
<td>15640</td>
+
−
<td>10000</td>
+
−
<td>5718</td>
+
−
<td>3073</td>
+
−
<td>1585</td>
+
−
<td>803</td>
+
−
<td>437</td>
+
−
<td>192</td>
+
−
<td>128</td>
+
−
<td>2.5</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Arithmetic Standard Deviation</th>
+
−
<td>172.8</td>
+
−
<td>1104</td>
+
−
<td>325.1</td>
+
−
<td>638.8</td>
+
−
<td>454.0</td>
+
−
<td>298.2</td>
+
−
<td>229.7</td>
+
−
<td>103.7</td>
+
−
<td>54.60</td>
+
−
<td>63.86</td>
+
−
<td>21.59</td>
+
−
<td>0.5774</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Arithmetic Net Mean</th>
+
−
<td>27666</td>
+
−
<td>21036</td>
+
−
<td>15637</td>
+
−
<td>9996</td>
+
−
<td>5716</td>
+
−
<td>3070</td>
+
−
<td>1582</td>
+
−
<td>801</td>
+
−
<td>435</td>
+
−
<td>190</td>
+
−
<td>126</td>
+
−
<td class="whiteCellsImportant"></td>
+
−
</tr>
+
−
</table>
+
−
<img src="https://static.igem.org/mediawiki/2018/2/29/T--UMaryland--interlab_flourescein_standard_curve.png" alt="interlab particle standard curve">
+
−
<img src="https://static.igem.org/mediawiki/2018/0/02/T--UMaryland--interlab_flourescein_standard_curve_log.png" alt="interlab particle standard curve">
+
−
<table id="flouresceinAU">
+
−
<tr>
+
−
<th>Flourescein µM</th>
+
−
<th>10.00</th>
+
−
<th>5.00</th>
+
−
<th>2.50</th>
+
−
<th>1.25</th>
+
−
<th>0.63</th>
+
−
<th>0.31</th>
+
−
<th>0.16</th>
+
−
<th>0.08</th>
+
−
<th>0.04</th>
+
−
<th>0.02</th>
+
−
<th>0.01</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">µM Fluorescein/a.u.</th>
+
−
<td>3.61×10<sup>-4</sup></td>
+
−
<td>2.38×10<sup>-4</sup></td>
+
−
<td>1.60×10<sup>-4</sup></td>
+
−
<td>1.25×10<sup>-4</sup></td>
+
−
<td>1.09×10<sup>-4</sup></td>
+
−
<td>1.02×10<sup>-4</sup></td>
+
−
<td>9.88×10<sup>-5</sup></td>
+
−
<td>9.76×10<sup>-5</sup></td>
+
−
<td>8.99×10<sup>-5</sup></td>
+
−
<td>1.03×10<sup>-4</sup></td>
+
−
<td>7.78×10<sup>-5</sup></td>
+
−
</tr>
+
−
<tr>
+
−
<th>Mean µM Fluorescein/a.u.</th>
+
−
<td></td>
+
−
<td class="yellowCells">1.47×10<sup>-4</sup></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
</tr>
+
−
<tr>
+
−
<th>MEFL/a.u.</th>
+
−
<td></td>
+
−
<td class="yellowCells">8.84×10<sup>8</sup></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
<td></td>
+
−
</tr>
+
−
</table>
+
 
</div>
 
</div>
−
<div id="Cell Measurement">
+
<div id="calibration">
−
<h2>Cell Measurement</h2>
+
<div class="meatSubtitle">
−
<div id="Raw Plate Reader Measurements">
+
1. Calibration
−
<h3>Plate Reader Raw Measurement</h3>
+
</div>
−
<h4>Fluorescence Raw Readings</h4>
+
<div class="meatMeat">
−
<h5>Hour 0:</h5>
+
OD600 Reference Point - LUDOX Protocol
−
<table id="FlourescenceRawReadingsHour0">
+
<br>
−
<tr>
+
<div class="center">
−
<th></th>
+
<img src="https://static.igem.org/mediawiki/2018/4/47/T--UMaryland--interlab1.png" alt="waluigi time">
−
<th>Negative Control</th>
+
</div>
−
<th>Positive Control</th>
+
</div>
−
<th>Device 1</th>
+
<div class="meatMeat">
−
<th>Device 2</th>
+
Particle Standard Curve - Microsphere Protocol
−
<th>Device 3</th>
+
<br>
−
<th>Device 4</th>
+
<div class="center">
−
<th>Device 5</th>
+
<img src="https://2018.igem.org/File:T--UMaryland--interlab2.png" alt="waluigi time">
−
<th>Device 6</th>
+
<img src="https://static.igem.org/mediawiki/2018/b/bb/T--UMaryland--interlab_particle_standard_curve.png" alt="interlab particle standard curve">
−
<th>LB + Chloro. (blank)</th>
+
<img src="https://static.igem.org/mediawiki/2018/7/77/T--UMaryland--interlab_particle_standard_curve_log.png" alt="interlab particle standard curve">
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2018/d/d6/T--UMaryland--interlab3.png" alt="waluigi time">
−
<tr class="blueCells">
+
</div>
−
<th class="whiteCellsImportant">Colony 1 Replicate 1</th>
+
</div>
−
<td>23</td>
+
<div class="meatMeat">
−
<td>43</td>
+
Fluorescence Standard Curve - Fluorescein Protocol
−
<td>63</td>
+
<br>
−
<td>44</td>
+
<div class="center">
−
<td>24</td>
+
<img src="https://static.igem.org/mediawiki/2018/8/89/T--UMaryland--interlab4.png" alt="waluigi time">
−
<td>84</td>
+
<img src="https://static.igem.org/mediawiki/2018/2/29/T--UMaryland--interlab_flourescein_standard_curve.png" alt="interlab particle standard curve">
−
<td>63</td>
+
<img src="https://static.igem.org/mediawiki/2018/0/02/T--UMaryland--interlab_flourescein_standard_curve_log.png" alt="interlab particle standard curve">
−
<td>55</td>
+
<img src="https://static.igem.org/mediawiki/2018/4/40/T--UMaryland--interlab5.png" alt="waluigi time">
−
<td>23</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 2</th>
+
−
<td>25</td>
+
−
<td>36</td>
+
−
<td>51</td>
+
−
<td>36</td>
+
−
<td>21</td>
+
−
<td>69</td>
+
−
<td>52</td>
+
−
<td>44</td>
+
−
<td>20</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 3</th>
+
−
<td>25</td>
+
−
<td>35</td>
+
−
<td>51</td>
+
−
<td>38</td>
+
−
<td>23</td>
+
−
<td>70</td>
+
−
<td>52</td>
+
−
<td>44</td>
+
−
<td>20</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 4</th>
+
−
<td>19</td>
+
−
<td>37</td>
+
−
<td>54</td>
+
−
<td>35</td>
+
−
<td>23</td>
+
−
<td>70</td>
+
−
<td>53</td>
+
−
<td>48</td>
+
−
<td>21</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 1</th>
+
−
<td>25</td>
+
−
<td>35</td>
+
−
<td>80</td>
+
−
<td>29</td>
+
−
<td>25</td>
+
−
<td>74</td>
+
−
<td>68</td>
+
−
<td>38</td>
+
−
<td>21</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 2</th>
+
−
<td>28</td>
+
−
<td>34</td>
+
−
<td>81</td>
+
−
<td>31</td>
+
−
<td>25</td>
+
−
<td>75</td>
+
−
<td>69</td>
+
−
<td>39</td>
+
−
<td>22</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 3</th>
+
−
<td>27</td>
+
−
<td>35</td>
+
−
<td>83</td>
+
−
<td>32</td>
+
−
<td>25</td>
+
−
<td>75</td>
+
−
<td>68</td>
+
−
<td>40</td>
+
−
<td>21</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 4</th>
+
−
<td>31</td>
+
−
<td>46</td>
+
−
<td>105</td>
+
−
<td>40</td>
+
−
<td>34</td>
+
−
<td>101</td>
+
−
<td>90</td>
+
−
<td>52</td>
+
−
<td>27</td>
+
−
</tr>
+
−
</table>
+
−
<h5>Hour 6:</h5>
+
−
<table id="FlourescenceRawReadingsHour6">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
<th>LB + Chloro. (blank)</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 1</th>
+
−
<td>31</td>
+
−
<td>288</td>
+
−
<td>447</td>
+
−
<td>251</td>
+
−
<td>37</td>
+
−
<td>132</td>
+
−
<td>111</td>
+
−
<td>205</td>
+
−
<td>24</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 2</th>
+
−
<td>36</td>
+
−
<td>266</td>
+
−
<td>382</td>
+
−
<td>213</td>
+
−
<td>36</td>
+
−
<td>112</td>
+
−
<td>93</td>
+
−
<td>186</td>
+
−
<td>20</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 3</th>
+
−
<td>35</td>
+
−
<td>252</td>
+
−
<td>382</td>
+
−
<td>216</td>
+
−
<td>37</td>
+
−
<td>108</td>
+
−
<td>102</td>
+
−
<td>178</td>
+
−
<td>21</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 4</th>
+
−
<td>36</td>
+
−
<td>260</td>
+
−
<td>381</td>
+
−
<td>216</td>
+
−
<td>39</td>
+
−
<td>113</td>
+
−
<td>93</td>
+
−
<td>179</td>
+
−
<td>22</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 1</th>
+
−
<td>36</td>
+
−
<td>260</td>
+
−
<td>381</td>
+
−
<td>216</td>
+
−
<td>39</td>
+
−
<td>116</td>
+
−
<td>140</td>
+
−
<td>161</td>
+
−
<td>24</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 2</th>
+
−
<td>39</td>
+
−
<td>215</td>
+
−
<td>340</td>
+
−
<td>152</td>
+
−
<td>110</td>
+
−
<td>121</td>
+
−
<td>137</td>
+
−
<td>163</td>
+
−
<td>24</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 3</th>
+
−
<td>37</td>
+
−
<td>210</td>
+
−
<td>348</td>
+
−
<td>150</td>
+
−
<td>108</td>
+
−
<td>117</td>
+
−
<td>139</td>
+
−
<td>161</td>
+
−
<td>23</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 4</th>
+
−
<td>41</td>
+
−
<td>258</td>
+
−
<td>415</td>
+
−
<td>175</td>
+
−
<td>130</td>
+
−
<td>150</td>
+
−
<td>183</td>
+
−
<td>198</td>
+
−
<td>23</td>
+
−
</tr>
+
−
</table>
+
−
<h4>Abs600 Raw Readings</h4>
+
−
<h5>Hour 0:</h5>
+
−
<table id="Abs600RawReadingsHour0">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
<th>LB + Chloro. (blank)</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 1</th>
+
−
<td>0.077</td>
+
−
<td>0.073</td>
+
−
<td>0.074</td>
+
−
<td>0.078</td>
+
−
<td>0.075</td>
+
−
<td>0.068</td>
+
−
<td>0.068</td>
+
−
<td>0.068</td>
+
−
<td>0.060</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 2</th>
+
−
<td>0.079</td>
+
−
<td>0.075</td>
+
−
<td>0.069</td>
+
−
<td>0.077</td>
+
−
<td>0.075</td>
+
−
<td>0.074</td>
+
−
<td>0.076</td>
+
−
<td>0.074</td>
+
−
<td>0.054</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 3</th>
+
−
<td>0.077</td>
+
−
<td>0.074</td>
+
−
<td>0.062</td>
+
−
<td>0.078</td>
+
−
<td>0.076</td>
+
−
<td>0.077</td>
+
−
<td>0.077</td>
+
−
<td>0.077</td>
+
−
<td>0.059</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 4</th>
+
−
<td>0.068</td>
+
−
<td>0.076</td>
+
−
<td>0.076</td>
+
−
<td>0.080</td>
+
−
<td>0.074</td>
+
−
<td>0.071</td>
+
−
<td>0.073</td>
+
−
<td>0.077</td>
+
−
<td>0.057</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 1</th>
+
−
<td>0.081</td>
+
−
<td>0.077</td>
+
−
<td>0.084</td>
+
−
<td>0.077</td>
+
−
<td>0.072</td>
+
−
<td>0.074</td>
+
−
<td>0.079</td>
+
−
<td>0.078</td>
+
−
<td>0.060</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 2</th>
+
−
<td>0.085</td>
+
−
<td>0.075</td>
+
−
<td>0.083</td>
+
−
<td>0.079</td>
+
−
<td>0.072</td>
+
−
<td>0.074</td>
+
−
<td>0.076</td>
+
−
<td>0.082</td>
+
−
<td>0.059</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 3</th>
+
−
<td>0.081</td>
+
−
<td>0.076</td>
+
−
<td>0.083</td>
+
−
<td>0.081</td>
+
−
<td>0.074</td>
+
−
<td>0.074</td>
+
−
<td>0.088</td>
+
−
<td>0.081</td>
+
−
<td>0.057</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 4</th>
+
−
<td>0.086</td>
+
−
<td>0.075</td>
+
−
<td>0.083</td>
+
−
<td>0.075</td>
+
−
<td>0.072</td>
+
−
<td>0.074</td>
+
−
<td>0.072</td>
+
−
<td>0.082</td>
+
−
<td>0.056</td>
+
−
</tr>
+
−
</table>
+
−
<h5>Hour 6:</h5>
+
−
<table id="Abs600RawReadingsHour6">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
<th>LB + Chloro. (blank)</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 1</th>
+
−
<td>0.445</td>
+
−
<td>0.437</td>
+
−
<td>0.258</td>
+
−
<td>0.447</td>
+
−
<td>0.487</td>
+
−
<td>0.108</td>
+
−
<td>0.329</td>
+
−
<td>0.459</td>
+
−
<td>0.062</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 2</th>
+
−
<td>0.456</td>
+
−
<td>0.475</td>
+
−
<td>0.261</td>
+
−
<td>0.444</td>
+
−
<td>0.518</td>
+
−
<td>0.140</td>
+
−
<td>0.324</td>
+
−
<td>0.477</td>
+
−
<td>0.059</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 3</th>
+
−
<td>0.473</td>
+
−
<td>0.433</td>
+
−
<td>0.270</td>
+
−
<td>0.450</td>
+
−
<td>0.524</td>
+
−
<td>0.109</td>
+
−
<td>0.353</td>
+
−
<td>0.450</td>
+
−
<td>0.063</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 1 Replicate 4</th>
+
−
<td>0.475</td>
+
−
<td>0.431</td>
+
−
<td>0.254</td>
+
−
<td>0.444</td>
+
−
<td>0.535</td>
+
−
<td>0.109</td>
+
−
<td>0.328</td>
+
−
<td>0.438</td>
+
−
<td>0.059</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 1</th>
+
−
<td>0.456</td>
+
−
<td>0.414</td>
+
−
<td>0.229</td>
+
−
<td>0.459</td>
+
−
<td>0.444</td>
+
−
<td>0.115</td>
+
−
<td>0.112</td>
+
−
<td>0.463</td>
+
−
<td>0.060</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 2</th>
+
−
<td>0.411</td>
+
−
<td>0.429</td>
+
−
<td>0.243</td>
+
−
<td>0.452</td>
+
−
<td>0.460</td>
+
−
<td>0.119</td>
+
−
<td>0.106</td>
+
−
<td>0.453</td>
+
−
<td>0.058</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 3</th>
+
−
<td>0.435</td>
+
−
<td>0.418</td>
+
−
<td>0.234</td>
+
−
<td>0.474</td>
+
−
<td>0.460</td>
+
−
<td>0.120</td>
+
−
<td>0.113</td>
+
−
<td>0.455</td>
+
−
<td>0.063</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Colony 2 Replicate 4</th>
+
−
<td>0.425</td>
+
−
<td>0.424</td>
+
−
<td>0.248</td>
+
−
<td>0.470</td>
+
−
<td>0.450</td>
+
−
<td>0.121</td>
+
−
<td>0.115</td>
+
−
<td>0.466</td>
+
−
<td>0.061</td>
+
−
</tr>
+
−
</table>
+
−
<h3>Fluoresence per OD</h3>
+
−
<h4>µM Flourescin/OD</h4>
+
−
<h5>Hour 0:</h5>
+
−
<table id="uMFlourescinODHour0">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>0.000</td>
+
−
<td>0.078</td>
+
−
<td>0.145</td>
+
−
<td>0.059</td>
+
−
<td>0.003</td>
+
−
<td>0.386</td>
+
−
<td>0.253</td>
+
−
<td>0.203</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>0.010</td>
+
−
<td>0.039</td>
+
−
<td>0.105</td>
+
−
<td>0.035</td>
+
−
<td>0.002</td>
+
−
<td>0.124</td>
+
−
<td>0.074</td>
+
−
<td>0.061</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>0.014</td>
+
−
<td>0.039</td>
+
−
<td>0.105</td>
+
−
<td>0.035</td>
+
−
<td>0.002</td>
+
−
<td>0.124</td>
+
−
<td>0.074</td>
+
−
<td>0.061</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>0.014</td>
+
−
<td>0.051</td>
+
−
<td>0.121</td>
+
−
<td>0.048</td>
+
−
<td>0.009</td>
+
−
<td>0.141</td>
+
−
<td>0.090</td>
+
−
<td>0.068</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>-0.009</td>
+
−
<td>0.043</td>
+
−
<td>0.088</td>
+
−
<td>0.031</td>
+
−
<td>0.006</td>
+
−
<td>0.177</td>
+
−
<td>0.101</td>
+
−
<td>0.068</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>0.010</td>
+
−
<td>0.042</td>
+
−
<td>0.125</td>
+
−
<td>0.023</td>
+
−
<td>0.012</td>
+
−
<td>0.179</td>
+
−
<td>0.140</td>
+
−
<td>0.037</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>0.013</td>
+
−
<td>0.037</td>
+
−
<td>0.121</td>
+
−
<td>0.023</td>
+
−
<td>0.012</td>
+
−
<td>0.161</td>
+
−
<td>0.077</td>
+
−
<td>0.040</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>0.007</td>
+
−
<td>0.051</td>
+
−
<td>0.146</td>
+
−
<td>0.035</td>
+
−
<td>0.022</td>
+
−
<td>0.208</td>
+
−
<td>0.199</td>
+
−
<td>0.049</td>
+
−
</tr>
+
−
</table>
+
−
<h5>Hour 6:</h5>
+
−
<table id="uMFlourescinODHour6">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>0.001</td>
+
−
<td>0.036</td>
+
−
<td>0.109</td>
+
−
<td>0.030</td>
+
−
<td>0.002</td>
+
−
<td>0.119</td>
+
−
<td>0.017</td>
+
−
<td>0.023</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>0.002</td>
+
−
<td>0.030</td>
+
−
<td>0.091</td>
+
−
<td>0.025</td>
+
−
<td>0.002</td>
+
−
<td>0.058</td>
+
−
<td>0.014</td>
+
−
<td>0.020</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>0.002</td>
+
−
<td>0.032</td>
+
−
<td>0.089</td>
+
−
<td>0.026</td>
+
−
<td>0.002</td>
+
−
<td>0.092</td>
+
−
<td>0.013</td>
+
−
<td>0.021</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>0.002</td>
+
−
<td>0.032</td>
+
−
<td>0.089</td>
+
−
<td>0.026</td>
+
−
<td>0.002</td>
+
−
<td>0.092</td>
+
−
<td>0.013</td>
+
−
<td>0.021</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>0.002</td>
+
−
<td>0.026</td>
+
−
<td>0.091</td>
+
−
<td>0.015</td>
+
−
<td>0.011</td>
+
−
<td>0.085</td>
+
−
<td>0.113</td>
+
−
<td>0.017</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>0.002</td>
+
−
<td>0.026</td>
+
−
<td>0.087</td>
+
−
<td>0.016</td>
+
−
<td>0.011</td>
+
−
<td>0.081</td>
+
−
<td>0.119</td>
+
−
<td>0.018</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>0.002</td>
+
−
<td>0.027</td>
+
−
<td>0.096</td>
+
−
<td>0.016</td>
+
−
<td>0.011</td>
+
−
<td>0.084</td>
+
−
<td>0.118</td>
+
−
<td>0.018</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>0.003</td>
+
−
<td>0.033</td>
+
−
<td>0.106</td>
+
−
<td>0.019</td>
+
−
<td>0.014</td>
+
−
<td>0.107</td>
+
−
<td>0.150</td>
+
−
<td>0.022</td>
+
−
</tr>
+
−
</table>
+
−
<h4>Net Flourescin a.u.</h4>
+
−
<h5>Hour 0:</h5>
+
−
<table id="netFluoresceinauHour0">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>0</td>
+
−
<td>20</td>
+
−
<td>40</td>
+
−
<td>21</td>
+
−
<td>1</td>
+
−
<td>61</td>
+
−
<td>40</td>
+
−
<td>32</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>5</td>
+
−
<td>16</td>
+
−
<td>31</td>
+
−
<td>16</td>
+
−
<td>1</td>
+
−
<td>49</td>
+
−
<td>32</td>
+
−
<td>24</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>5</td>
+
−
<td>15</td>
+
−
<td>31</td>
+
−
<td>18</td>
+
−
<td>3</td>
+
−
<td>50</td>
+
−
<td>32</td>
+
−
<td>24</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>-2</td>
+
−
<td>16</td>
+
−
<td>33</td>
+
−
<td>14</td>
+
−
<td>2</td>
+
−
<td>49</td>
+
−
<td>32</td>
+
−
<td>27</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>4</td>
+
−
<td>14</td>
+
−
<td>59</td>
+
−
<td>8</td>
+
−
<td>4</td>
+
−
<td>53</td>
+
−
<td>47</td>
+
−
<td>17</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>6</td>
+
−
<td>12</td>
+
−
<td>59</td>
+
−
<td>9</td>
+
−
<td>3</td>
+
−
<td>53</td>
+
−
<td>47</td>
+
−
<td>17</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>6</td>
+
−
<td>14</td>
+
−
<td>62</td>
+
−
<td>11</td>
+
−
<td>4</td>
+
−
<td>54</td>
+
−
<td>47</td>
+
−
<td>19</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>4</td>
+
−
<td>14</td>
+
−
<td>78</td>
+
−
<td>13</td>
+
−
<td>7</td>
+
−
<td>74</td>
+
−
<td>63</td>
+
−
<td>25</td>
+
−
</tr>
+
−
</table>
+
−
<h5>Hour 6:</h5>
+
−
<table id="netFluoresceinauHour6">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>7</td>
+
−
<td>264</td>
+
−
<td>423</td>
+
−
<td>227</td>
+
−
<td>13</td>
+
−
<td>108</td>
+
−
<td>87</td>
+
−
<td>181</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>16</td>
+
−
<td>261</td>
+
−
<td>263</td>
+
−
<td>193</td>
+
−
<td>16</td>
+
−
<td>92</td>
+
−
<td>73</td>
+
−
<td>166</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>14</td>
+
−
<td>231</td>
+
−
<td>362</td>
+
−
<td>195</td>
+
−
<td>16</td>
+
−
<td>87</td>
+
−
<td>81</td>
+
−
<td>157</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>14</td>
+
−
<td>238</td>
+
−
<td>359</td>
+
−
<td>194</td>
+
−
<td>17</td>
+
−
<td>91</td>
+
−
<td>71</td>
+
−
<td>157</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>12</td>
+
−
<td>184</td>
+
−
<td>305</td>
+
−
<td>121</td>
+
−
<td>81</td>
+
−
<td>92</td>
+
−
<td>116</td>
+
−
<td>137</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>15</td>
+
−
<td>191</td>
+
−
<td>316</td>
+
−
<td>128</td>
+
−
<td>86</td>
+
−
<td>97</td>
+
−
<td>113</td>
+
−
<td>139</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>14</td>
+
−
<td>187</td>
+
−
<td>325</td>
+
−
<td>127</td>
+
−
<td>85</td>
+
−
<td>94</td>
+
−
<td>116</td>
+
−
<td>138</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>18</td>
+
−
<td>235</td>
+
−
<td>392</td>
+
−
<td>152</td>
+
−
<td>107</td>
+
−
<td>127</td>
+
−
<td>160</td>
+
−
<td>175</td>
+
−
</tr>
+
−
</table>
+
−
<div id="Net Abs 600">
+
−
<h5>Hour 0:</h5>
+
−
<table id="netAbs600Hour0">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>0.017</td>
+
−
<td>0.013</td>
+
−
<td>0.014</td>
+
−
<td>0.018</td>
+
−
<td>0.015</td>
+
−
<td>0.008</td>
+
−
<td>0.008</td>
+
−
<td>0.008</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>0.025</td>
+
−
<td>0.021</td>
+
−
<td>0.015</td>
+
−
<td>0.023</td>
+
−
<td>0.021</td>
+
−
<td>0.020</td>
+
−
<td>0.022</td>
+
−
<td>0.020</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>0.018</td>
+
−
<td>0.015</td>
+
−
<td>0.013</td>
+
−
<td>0.019</td>
+
−
<td>0.017</td>
+
−
<td>0.018</td>
+
−
<td>0.018</td>
+
−
<td>0.018</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>0.011</td>
+
−
<td>0.019</td>
+
−
<td>0.019</td>
+
−
<td>0.023</td>
+
−
<td>0.017</td>
+
−
<td>0.014</td>
+
−
<td>0.016</td>
+
−
<td>0.020</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>0.021</td>
+
−
<td>0.017</td>
+
−
<td>0.024</td>
+
−
<td>0.017</td>
+
−
<td>0.012</td>
+
−
<td>0.014</td>
+
−
<td>0.019</td>
+
−
<td>0.018</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>0.026</td>
+
−
<td>0.016</td>
+
−
<td>0.024</td>
+
−
<td>0.020</td>
+
−
<td>0.013</td>
+
−
<td>0.015</td>
+
−
<td>0.017</td>
+
−
<td>0.023</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>0.024</td>
+
−
<td>0.019</td>
+
−
<td>0.026</td>
+
−
<td>0.024</td>
+
−
<td>0.017</td>
+
−
<td>0.017</td>
+
−
<td>0.031</td>
+
−
<td>0.024</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>0.030</td>
+
−
<td>0.019</td>
+
−
<td>0.027</td>
+
−
<td>0.019</td>
+
−
<td>0.016</td>
+
−
<td>0.018</td>
+
−
<td>0.016</td>
+
−
<td>0.026</td>
+
−
</tr>
+
−
</table>
+
−
<h5>Hour 6:</h5>
+
−
<table id="netAbs600Hour6">
+
−
<tr>
+
−
<th></th>
+
−
<th>Negative Control</th>
+
−
<th>Positive Control</th>
+
−
<th>Device 1</th>
+
−
<th>Device 2</th>
+
−
<th>Device 3</th>
+
−
<th>Device 4</th>
+
−
<th>Device 5</th>
+
−
<th>Device 6</th>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
+
−
<td>0.383</td>
+
−
<td>0.375</td>
+
−
<td>0.196</td>
+
−
<td>0.385</td>
+
−
<td>0.425</td>
+
−
<td>0.046</td>
+
−
<td>0.267</td>
+
−
<td>0.397</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
+
−
<td>0.397</td>
+
−
<td>0.416</td>
+
−
<td>0.202</td>
+
−
<td>0.385</td>
+
−
<td>0.459</td>
+
−
<td>0.081</td>
+
−
<td>0.265</td>
+
−
<td>0.418</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
+
−
<td>0.410</td>
+
−
<td>0.370</td>
+
−
<td>0.207</td>
+
−
<td>0.387</td>
+
−
<td>0.461</td>
+
−
<td>0.046</td>
+
−
<td>0.290</td>
+
−
<td>0.387</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
+
−
<td>0.416</td>
+
−
<td>0.372</td>
+
−
<td>0.195</td>
+
−
<td>0.385</td>
+
−
<td>0.476</td>
+
−
<td>0.050</td>
+
−
<td>0.269</td>
+
−
<td>0.379</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
+
−
<td>0.396</td>
+
−
<td>0.354</td>
+
−
<td>0.169</td>
+
−
<td>0.399</td>
+
−
<td>0.384</td>
+
−
<td>0.055</td>
+
−
<td>0.052</td>
+
−
<td>0.403</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
+
−
<td>0.353</td>
+
−
<td>0.371</td>
+
−
<td>0.185</td>
+
−
<td>0.394</td>
+
−
<td>0.402</td>
+
−
<td>0.061</td>
+
−
<td>0.048</td>
+
−
<td>0.395</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
+
−
<td>0.372</td>
+
−
<td>0.355</td>
+
−
<td>0.171</td>
+
−
<td>0.411</td>
+
−
<td>0.397</td>
+
−
<td>0.057</td>
+
−
<td>0.050</td>
+
−
<td>0.392</td>
+
−
</tr>
+
−
<tr class="yellowCells">
+
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
+
−
<td>0.364</td>
+
−
<td>0.363</td>
+
−
<td>0.187</td>
+
−
<td>0.409</td>
+
−
<td>0.389</td>
+
−
<td>0.060</td>
+
−
<td>0.054</td>
+
−
<td>0.405</td>
+
−
</tr>
+
−
</table>
+
 
</div>
 
</div>
 
</div>
 
</div>
−
<h3>Fluorescence per Particle</h3>
 
−
<h4>MEFL/particle</h4>
 
−
<h5>Hour 0:</h5>
 
−
<table id="MEFLParticleHour0">
 
−
<tr>
 
−
<th></th>
 
−
<th>Negative Control</th>
 
−
<th>Positive Control</th>
 
−
<th>Device 1</th>
 
−
<th>Device 2</th>
 
−
<th>Device 3</th>
 
−
<th>Device 4</th>
 
−
<th>Device 5</th>
 
−
<th>Device 6</th>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
 
−
<td>0</td>
 
−
<td>3.67×10<sup>3</sup></td>
 
−
<td>6.82×10<sup>3</sup></td>
 
−
<td>2.79×10<sup>3</sup></td>
 
−
<td>1.59×10<sup>2</sup></td>
 
−
<td>1.82×10<sup>4</sup></td>
 
−
<td>1.19×10<sup>4</sup></td>
 
−
<td>9.55×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
 
−
<td>4.78×10<sup>2</sup></td>
 
−
<td>1.82×10<sup>3</sup></td>
 
−
<td>4.93×10<sup>3</sup></td>
 
−
<td>1.66×10<sup>3</sup></td>
 
−
<td>1.14×10<sup>2</sup></td>
 
−
<td>5.85×10<sup>3</sup></td>
 
−
<td>3.47×10<sup>3</sup></td>
 
−
<td>2.87×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
 
−
<td>6.63×10<sup>2</sup></td>
 
−
<td>2.39×10<sup>3</sup></td>
 
−
<td>5.69×10<sup>3</sup></td>
 
−
<td>2.26×10<sup>3</sup></td>
 
−
<td>4.21×10<sup>2</sup></td>
 
−
<td>6.63×10<sup>3</sup></td>
 
−
<td>4.24×10<sup>3</sup></td>
 
−
<td>3.18×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
 
−
<td>-4.34×10<sup>2</sup></td>
 
−
<td>2.01×10<sup>3</sup></td>
 
−
<td>4.15×10<sup>3</sup></td>
 
−
<td>1.45×10<sup>3</sup></td>
 
−
<td>2.81×10<sup>2</sup></td>
 
−
<td>8.36×10<sup>3</sup></td>
 
−
<td>4.78×10<sup>3</sup></td>
 
−
<td>3.22×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
 
−
<td>4.55×10<sup>2</sup></td>
 
−
<td>1.97×10<sup>3</sup></td>
 
−
<td>5.87×10<sup>3</sup></td>
 
−
<td>1.12×10<sup>3</sup></td>
 
−
<td>7.96×10<sup>2</sup></td>
 
−
<td>9.04×10<sup>3</sup></td>
 
−
<td>5.91×10<sup>3</sup></td>
 
−
<td>2.26×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
 
−
<td>5.51×10<sup>2</sup></td>
 
−
<td>1.79×10<sup>3</sup></td>
 
−
<td>5.87×10<sup>3</sup></td>
 
−
<td>1.07×10<sup>3</sup></td>
 
−
<td>5.51×10<sup>2</sup></td>
 
−
<td>8.44×10<sup>3</sup></td>
 
−
<td>6.60×10<sup>3</sup></td>
 
−
<td>1.76×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
 
−
<td>5.97×10<sup>2</sup></td>
 
−
<td>1.76×10<sup>3</sup></td>
 
−
<td>5.69×10<sup>3</sup></td>
 
−
<td>1.09×10<sup>3</sup></td>
 
−
<td>5.62×10<sup>2</sup></td>
 
−
<td>7.58×10<sup>3</sup></td>
 
−
<td>3.62×10<sup>3</sup></td>
 
−
<td>1.89×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
 
−
<td>3.18×10<sup>2</sup></td>
 
−
<td>2.39×10<sup>3</sup></td>
 
−
<td>6.90×10<sup>3</sup></td>
 
−
<td>1.63×10<sup>3</sup></td>
 
−
<td>1.04×10<sup>3</sup></td>
 
−
<td>9.82×10<sup>3</sup></td>
 
−
<td>9.40×10<sup>3</sup></td>
 
−
<td>2.03×10<sup>3</sup></td>
 
−
</tr>
 
−
</table>
 
−
<h5>Hour 6:</h5>
 
−
<table id="MEFLParticleHour6">
 
−
<tr>
 
−
<th></th>
 
−
<th>Negative Control</th>
 
−
<th>Positive Control</th>
 
−
<th>Device 1</th>
 
−
<th>Device 2</th>
 
−
<th>Device 3</th>
 
−
<th>Device 4</th>
 
−
<th>Device 5</th>
 
−
<th>Device 6</th>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 1</th>
 
−
<td>4.36×10<sup>1</sup></td>
 
−
<td>1.68×10<sup>3</sup></td>
 
−
<td>5.15×10<sup>3</sup></td>
 
−
<td>1.41×10<sup>3</sup></td>
 
−
<td>7.30×10<sup>1</sup></td>
 
−
<td>5.61×10<sup>3</sup></td>
 
−
<td>7.78×10<sup>2</sup></td>
 
−
<td>1.09×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 2</th>
 
−
<td>9.62×10<sup>1</sup></td>
 
−
<td>1.41×10<sup>3</sup></td>
 
−
<td>2.28×10<sup>3</sup></td>
 
−
<td>1.20×10<sup>3</sup></td>
 
−
<td>8.32×10<sup>1</sup></td>
 
−
<td>2.71×10<sup>3</sup></td>
 
−
<td>6.58×10<sup>2</sup></td>
 
−
<td>9.48×10<sup>2</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 3</th>
 
−
<td>8.15×10<sup>1</sup></td>
 
−
<td>1.49×10<sup>3</sup></td>
 
−
<td>4.18×10<sup>3</sup></td>
 
−
<td>1.20×10<sup>3</sup></td>
 
−
<td>8.29×10<sup>1</sup></td>
 
−
<td>4.52×10<sup>3</sup></td>
 
−
<td>6.67×10<sup>2</sup></td>
 
−
<td>9.69×10<sup>2</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 1, Replicate 4</th>
 
−
<td>8.04×10<sup>1</sup></td>
 
−
<td>1.53×10<sup>3</sup></td>
 
−
<td>4.40×10<sup>3</sup></td>
 
−
<td>1.20×10<sup>3</sup></td>
 
−
<td>8.53×10<sup>2</sup></td>
 
−
<td>4.35×10<sup>3</sup></td>
 
−
<td>6.30×10<sup>2</sup></td>
 
−
<td>9.89×10<sup>3</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 1</th>
 
−
<td>7.24×10<sup>1</sup></td>
 
−
<td>1.24×10<sup>3</sup></td>
 
−
<td>4.31×10<sup>3</sup></td>
 
−
<td>7.24×10<sup>2</sup></td>
 
−
<td>5.04×10<sup>2</sup></td>
 
−
<td>3.99×10<sup>3</sup></td>
 
−
<td>5.33×10<sup>3</sup></td>
 
−
<td>8.12×10<sup>2</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 2</th>
 
−
<td>1.01×10<sup>2</sup></td>
 
−
<td>1.23×10<sup>3</sup></td>
 
−
<td>4.08×10<sup>3</sup></td>
 
−
<td>7.76×10<sup>2</sup></td>
 
−
<td>5.11×10<sup>2</sup></td>
 
−
<td>3.80×10<sup>3</sup></td>
 
−
<td>5.62×10<sup>3</sup></td>
 
−
<td>8.40×10<sup>2</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 3</th>
 
−
<td>8.99×10<sup>1</sup></td>
 
−
<td>1.26×10<sup>3</sup></td>
 
−
<td>4.54×10<sup>3</sup></td>
 
−
<td>7.38×10<sup>2</sup></td>
 
−
<td>5.11×10<sup>2</sup></td>
 
−
<td>3.94×10<sup>3</sup></td>
 
−
<td>5.54×10<sup>3</sup></td>
 
−
<td>8.41×10<sup>2</sup></td>
 
−
</tr>
 
−
<tr class="yellowCells">
 
−
<th class="whiteCellsImportant">Colony 2, Replicate 4</th>
 
−
<td>1.18×10<sup>2</sup></td>
 
−
<td>1.55×10<sup>3</sup></td>
 
−
<td>5.01×10<sup>3</sup></td>
 
−
<td>8.87×10<sup>2</sup></td>
 
−
<td>6.57×10<sup>2</sup></td>
 
−
<td>5.05×10<sup>3</sup></td>
 
−
<td>7.07×10<sup>3</sup></td>
 
−
<td>1.03×10<sup>3</sup></td>
 
−
</tr>
 
−
</table>
 
 
</div>
 
</div>
−
<div id="Colony Forming Units">
+
<div id="cellmeasurment">
−
<h2>Colony Forming Units per 0.1 OD600 <i>E. coli</i> Cultures</h2>
+
<div class="meatSubtitle">
−
<div id="coloniesPerPlate">
+
2. Cell Measurement
−
<h3>Colonies per Plate</h3>
+
−
<h4>Positive Control</h4>
+
−
<table id="coloniesPerPlatePositive">
+
−
<tr>
+
−
<th></th>
+
−
<th>Colony 1, Replicate 1</th>
+
−
<th>Colony 1, Replicate 2</th>
+
−
<th>Colony 1, Replicate 3</th>
+
−
<th>Colony 2, Replicate 1</th>
+
−
<th>Colony 2, Replicate 2</th>
+
−
<th>Colony 2, Replicate 3</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 3</th>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 4</th>
+
−
<td>64</td>
+
−
<td>129</td>
+
−
<td>127</td>
+
−
<td>9</td>
+
−
<td>143</td>
+
−
<td>80</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 5</th>
+
−
<td>13</td>
+
−
<td>8</td>
+
−
<td>12</td>
+
−
<td>5</td>
+
−
<td>18</td>
+
−
<td>5</td>
+
−
</tr>
+
−
</table>
+
−
<h4>Negative Control</h4>
+
−
<table id="coloniesPerPlateNegative">
+
−
<tr>
+
−
<th></th>
+
−
<th>Colony 1, Replicate 1</th>
+
−
<th>Colony 1, Replicate 2</th>
+
−
<th>Colony 1, Replicate 3</th>
+
−
<th>Colony 2, Replicate 1</th>
+
−
<th>Colony 2, Replicate 2</th>
+
−
<th>Colony 2, Replicate 3</th>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 3</th>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>157</td>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 4</th>
+
−
<td>>300</td>
+
−
<td>>300</td>
+
−
<td>92</td>
+
−
<td>259</td>
+
−
<td>5</td>
+
−
<td>3</td>
+
−
</tr>
+
−
<tr class="blueCells">
+
−
<th class="whiteCellsImportant">Dilution 5</th>
+
−
<td>41</td>
+
−
<td>33</td>
+
−
<td>14</td>
+
−
<td>11</td>
+
−
<td>0</td>
+
−
<td>0</td>
+
−
</tr>
+
−
</table>
+
 
</div>
 
</div>
−
<div id="colonyFormingUnitspermL">
+
<div class="meatMeat">
−
<h3>Colony Forming Units per mL</h3>
+
Plate Reader Raw Measurement
−
<h4>Positive Control</h4>
+
<br>
−
<table id="colonyFormingUnitspermlPoistive">
+
Fluorescence Raw Readings
−
<tr>
+
<br>
−
<th></th>
+
Hour 0:
−
<th>Colony 1, Replicate 1</th>
+
<br>
−
<th>Colony 1, Replicate 2</th>
+
<div class="center">
−
<th>Colony 1, Replicate 3</th>
+
<img src="https://static.igem.org/mediawiki/2018/d/de/T--UMaryland--interlab6.png" alt="waluigi time">
−
<th>Colony 2, Replicate 1</th>
+
</div>
−
<th>Colony 2, Replicate 2</th>
+
Hour 6:
−
<th>Colony 2, Replicate 3</th>
+
<br>
−
</tr>
+
<div class="center">
−
<tr class="yellowCells">
+
<img src="https://static.igem.org/mediawiki/2018/1/16/T--UMaryland--interlab7.png" alt="waluigi time">
−
<th class="whiteCellsImportant">Dilution 3</th>
+
</div>
−
<td>-</td>
+
<br>
−
<td>-</td>
+
Abs600 Raw Readings
−
<td>-</td>
+
<br>
−
<td>-</td>
+
Hour 0:
−
<td>-</td>
+
<br>
−
<td>-</td>
+
<div class="center">
−
</tr>
+
<img src="https://static.igem.org/mediawiki/2018/8/88/T--UMaryland--interlab8.png" alt="waluigi time">
−
<tr class="yellowCells">
+
</div>
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<th class="whiteCellsImportant">Dilution 4</th>
+
Hour 6:
−
<td>5.12×10<sup>7</sup></td>
+
<br>
−
<td>1.03×10<sup>8</sup></td>
+
<div class="center">
−
<td>1.02×10<sup>8</sup></td>
+
<img src="https://static.igem.org/mediawiki/2018/5/5d/T--UMaryland--interlab9.png" alt="waluigi time">
−
<td>7.20×10<sup>6</sup></td>
+
</div>
−
<td>1.14×10<sup>8</sup></td>
+
<br>
−
<td>6.40×10<sup>7</sup></td>
+
Fluorescence per OD
−
</tr>
+
<br>
−
<tr class="yellowCells">
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µM Flourescin/OD
−
<th class="whiteCellsImportant">Dilution 5</th>
+
<br>
−
<td>1.04×10<sup>8</sup></td>
+
Hour 0:
−
<td>6.40×10<sup>7</sup></td>
+
<br>
−
<td>9.60×10<sup>7</sup></td>
+
<div class="center">
−
<td>4.00×10<sup>7</sup></td>
+
<img src="https://static.igem.org/mediawiki/2018/4/4f/T--UMaryland--interlab10.png" alt="waluigi time">
−
<td>1.44×10<sup>8</sup></td>
+
</div>
−
<td>4.00×10<sup>7</sup></td>
+
Hour 6:
−
</tr>
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<br>
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</table>
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<div class="center">
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<h4>Negative Control</h4>
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<img src="https://static.igem.org/mediawiki/2018/2/2f/T--UMaryland--interlab11.png" alt="waluigi time">
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<table id="colonyFormingUnitspermlPositive">
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<tr>
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<br>
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<th></th>
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Net Fluorescin a.u.
−
<th>Colony 1, Replicate 1</th>
+
<br>
−
<th>Colony 1, Replicate 2</th>
+
Hour 0:
−
<th>Colony 1, Replicate 3</th>
+
<br>
−
<th>Colony 2, Replicate 1</th>
+
<div class="center">
−
<th>Colony 2, Replicate 2</th>
+
<img src="https://static.igem.org/mediawiki/2018/b/b0/T--UMaryland--interlab12.png" alt="waluigi time">
−
<th>Colony 2, Replicate 3</th>
+
</div>
−
</tr>
+
Hour 6:
−
<tr class="yellowCells">
+
<br>
−
<th class="whiteCellsImportant">Dilution 3</th>
+
<div class="center">
−
<td>-</td>
+
<img src="https://static.igem.org/mediawiki/2018/f/ff/T--UMaryland--interlab13.png" alt="waluigi time">
−
<td>-</td>
+
</div>
−
<td>-</td>
+
Hour 0:
−
<td>1.26×10<sup>7</sup></td>
+
<br>
−
<td>-</td>
+
<div class="center">
−
<td>-</td>
+
<img src="https://static.igem.org/mediawiki/2018/4/49/T--UMaryland--interlab14.png" alt="waluigi time">
−
</tr>
+
</div>
−
<tr class="yellowCells">
+
Hour 6:
−
<th class="whiteCellsImportant">Dilution 4</th>
+
<br>
−
<td>-</td>
+
<div class="center">
−
<td>-</td>
+
<img src="https://static.igem.org/mediawiki/2018/0/03/T--UMaryland--interlab15.png" alt="waluigi time">
−
<td>7.36×10<sup>7</sup></td>
+
</div>
−
<td>2.07×10<sup>8</sup></td>
+
<br>
−
<td>4.00×10<sup>6</sup></td>
+
Fluorescence per Particle
−
<td>2.40×10<sup>6</sup></td>
+
<br>
−
</tr>
+
MEFL/Particle
−
<tr class="yellowCells">
+
<br>
−
<th class="whiteCellsImportant">Dilution 5</th>
+
Hour 0:
−
<td>3.28×10<sup>8</sup></td>
+
<br>
−
<td>2.64×10<sup>8</sup></td>
+
<div class="center">
−
<td>1.12×10<sup>8</sup></td>
+
<img src="https://static.igem.org/mediawiki/2018/e/e5/T--UMaryland--interlab16.png" alt="waluigi time">
−
<td>8.80×10<sup>7</sup></td>
+
</div>
−
<td>0</td>
+
Hour 6:
−
<td>0</td>
+
<br>
−
</tr>
+
<div class="center">
−
</table>
+
<img src="https://static.igem.org/mediawiki/2018/4/43/T--UMaryland--interlab17.png" alt="waluigi time">
 +
</div>
 
</div>
 
</div>
 
</div>
 
</div>
−
<div id="Misc. Info">
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<div id="cfus">
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<h2>Miscellaneous Information</h2>
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<div class="meatSubtitle">
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<p>The 96 well plate we used was clear. Some of the flourescence data might be affected due to bleeding of flourescence from certain wells to another.</p>
+
3. CFUs per 0.1 OD600 <i>E. coli</i> Cultures
 +
</div>
 +
<div class="meatMeat">
 +
Colonies Per Plate
 +
<br>
 +
Positive Control
 +
<br>
 +
<div class="center">
 +
<img src="https://static.igem.org/mediawiki/2018/7/73/T--UMaryland--interlab18.png" alt="waluigi time">
 +
</div>
 +
Negative Control
 +
<br>
 +
<div class="center">
 +
<img src="https://static.igem.org/mediawiki/2018/7/74/T--UMaryland--interlab19.png" alt="waluigi time">
 +
</div>
 +
<br>
 +
Colony Forming Units per mL
 +
<br>
 +
Positive Control
 +
<br>
 +
<div class="center">
 +
<img src="https://static.igem.org/mediawiki/2018/1/11/T--UMaryland--interlab20.png" alt="waluigi time">
 +
</div>
 +
Negative Control
 +
<br>
 +
<div class="center">
 +
<img src="https://static.igem.org/mediawiki/2018/8/8f/T--UMaryland--interlab21.png" alt="waluigi time">
 +
</div>
 +
</div>
 +
</div>
 +
<div id="Discussion">
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<div class="meatSubtitle">
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Discussion
 +
</div>
 +
<div class="meatMeat">
 +
Our results indicate an increase in absorbance and fluorescence from 0 hours to 6 hours which indicates that there were more cells present after 6 hours at growth which contributed to a higher fluorescence. In particular, Test Device 1 had the highest fluorescence at both time points. Our positive and negative controls worked as intended and there was very little fluorescence detected from the negative control despite high absorbance indicating the presence of many cells. On average, there was a slight decrease in average fluorescence per molecule and average fluorescence per OD when comparing the 0 hour time point to the 6 hour time point, but it was mainly consistent. The individual cells did not become more or less fluorescent based on cellular concentration in the media.
 +
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<p>yeah we realize this is not so pretty. we're working on it.</p>
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<p>contact us at <a href="mailto:umarylandigem@gmail.com">umarylandigem@gmail.com</a></p>
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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Latest revision as of 03:58, 18 October 2018

Template Title Template Title

InterLab
Synthetic biology is all about collaboration between scientists in an attempt to further our understanding of the field and improve the world around us. However, with the variability that occurs in synthetic biology measurement, it is much harder to consistently build on work done by others. This year, the 5th Interlab Study was designed to reduce lab to lab variability in fluorescence by normalizing data to Colony Forming Units (CFU). The 2018 UMaryland iGEM team has performed the interlab study using the protocol distributed by the Measurement Committee.

Feel free to download our data here.
Procedures
All measurements were performed using a standard clear 96-well plate. Calibration of our microplate reader used LUDOX CL-X as a single point reference to appropriately convert absorbance values into a standard OD600 measurement. Absorbances were measured for four replicates per LUDOX and deionized water at 600 nm. The particle standard curve was produced using silica beads and measured at 600 nm. There was also a fluorescence standard curve produced by using fluorescein and 1xPBS solution. Once all calibrations were completed, transformations of all devices in DH5-α, including positive and negative controls took place. Two colonies were chosen from each and incubated in 5 mL of LB media with chloramphenicol. They were left overnight for 16 hours at 37 C at around 220 rpm. All samples were immediately placed on ice and 100 ul was pipetted into the 96 well plate at the 0 and 6h timepoints. The absorbance and fluorescence was measured for both. The CFU measurements were taken by diluting the overnight cultures to an OD of 0.1. The samples were then diluted 1:20 three times and then 1:10 another two times. The final three dilutions for each replicate were plated and colonies were counted after 18 hours of growth at 37 C to determine the CFU/ml as can be seen in our results section.
1. Calibration
OD600 Reference Point - LUDOX Protocol
waluigi time
Particle Standard Curve - Microsphere Protocol
waluigi time interlab particle standard curve interlab particle standard curve waluigi time
Fluorescence Standard Curve - Fluorescein Protocol
waluigi time interlab particle standard curve interlab particle standard curve waluigi time
2. Cell Measurement
Plate Reader Raw Measurement
Fluorescence Raw Readings
Hour 0:
waluigi time
Hour 6:
waluigi time

Abs600 Raw Readings
Hour 0:
waluigi time
Hour 6:
waluigi time

Fluorescence per OD
µM Flourescin/OD
Hour 0:
waluigi time
Hour 6:
waluigi time

Net Fluorescin a.u.
Hour 0:
waluigi time
Hour 6:
waluigi time
Hour 0:
waluigi time
Hour 6:
waluigi time

Fluorescence per Particle
MEFL/Particle
Hour 0:
waluigi time
Hour 6:
waluigi time
3. CFUs per 0.1 OD600 E. coli Cultures
Colonies Per Plate
Positive Control
waluigi time
Negative Control
waluigi time

Colony Forming Units per mL
Positive Control
waluigi time
Negative Control
waluigi time
Discussion
Our results indicate an increase in absorbance and fluorescence from 0 hours to 6 hours which indicates that there were more cells present after 6 hours at growth which contributed to a higher fluorescence. In particular, Test Device 1 had the highest fluorescence at both time points. Our positive and negative controls worked as intended and there was very little fluorescence detected from the negative control despite high absorbance indicating the presence of many cells. On average, there was a slight decrease in average fluorescence per molecule and average fluorescence per OD when comparing the 0 hour time point to the 6 hour time point, but it was mainly consistent. The individual cells did not become more or less fluorescent based on cellular concentration in the media.

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